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IPK Gatersleben > Research > Dept. Cytogenetics and Genome Analysis > Genome Plasticity
 

 

Genome Plasticity
Head: Dr Renate Schmidt
Tel: +49 (0)39482 5591
Fax: +49 (0)39482 5137
Email: schmidtr@ipk-gatersleben.de
Research Interest
The impact of duplications in the oilseed rape genome
Polyploidy is widespread in the plant kingdom. We are therefore interested to trace the fate of duplicated regions in the course of evolution. We use the allopolyploid species Brassica napus (oilseed rape) to study the arrangement, structure and expression of duplicated genes in selected regions of the rapeseed genome. A PCR-based screening platform of an oilseed rape BAC library enables quick access to regions of interest in the rapeseed genome. Furthermore, we determine the allelic diversity of selected genes in a broad set of oilseed rape accessions.

Genetic analysis of seed traits in Arabidopsis thaliana
We use the natural occurring variation in Arabidopsis thaliana accessions to study the genetic factors of importance for the trait seed yield. We determine characters such as seed weight, seed size, seed number per silique and seed yield per plant in a population of recombinant inbred lines and in a broad set of Arabidopsis thaliana accessions. QTL mapping and association studies are used to identify loci of importance for these traits.

Transgene silencing in Arabidopsis thaliana
Silencing of introduced genes is often observed in transgenic plants. A systematic analysis of transgene expression in Arabidopsis thaliana showed that transgene silencing is triggered by excessive transcript levels. The transgene expression level correlates with the probability that silencing is initiated early in plant development. The study of progeny of plants with silenced transgenes showed that the silenced state is not transmitted to the next generation, instead silencing has to be established in each generation anew. We are studying now how different environmental factors and genetic backgrounds affect the onset and/or the spread of silencing.
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Recent References
201220112010200920082007200620052004
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2011 SCHMIDT, R. & I. BANCROFT (EDS.) Genetics and Genomics of the Brassicaceae. Plant Genetics and Genomics: Crops and Models, Vol. 9. Springer-Verlag New York Dordrecht Heidelberg London.
2011 SCHMIDT, R. & I. BANCROFT Perspectives on genetics and genomics of the Brassicaceae. In: SCHMIDT, R. & I. BANCROFT (Eds.): Genetics and Genomics of the Brassicaceae. Springer-Verlag New York Dordrecht Heidelberg London, pp. 617-632.
2011 TOWN, C., R. SCHMIDT & I. BANCROFT Comparative genome analysis at the sequence level in the Brassicaceae. In: SCHMIDT, R. & I. BANCROFT (Eds.): Genetics and Genomics of the Brassicaceae. Springer-Verlag New York Dordrecht Heidelberg London, 171-194.
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2008 FISCHER, U., M. KUHLMANN, A. PECINKA, R. SCHMIDT & M.F. METTE Local DNA features affect RNA-directed transcriptional gene silencing and DNA methylation. Plant J. 53: 1-10.
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2007 ECKE W., M. LANGE, R. SCHMIDT, K. BACH, E. DIETRICH*, R. SNOWDON, K. LINK, M. OUZUNOVA, F. BREUER, D. STELLING, D. HAUSKA, G. LECKBAND, F. DREYER, J. DETERING, P. DUSCHERER, W. HORN & S. TUVESSON GABI-Bridge: Die Brücke von Sequenz zu Ölgehalt und Resistenz: DNA-Unterschiede kontrollieren agronomisch wichtige Merkmale bei Raps. In: Die Highlights aus der zweiten GABI Förderperiode. GenomXPress Sonderausgabe 1/07, 32.
2007 NASRALLAH, J.B., P. LIU, S. SHERMAN-BROYLES, R. SCHMIDT & M.E. NASRALLAH Epigenetic mechanisms for breakdown of self-incompatibility in inter-specific hybrids. Genetics 175: 1965-1973.
[ ^ ] 2006
2006 BANCROFT, I., S. BARNES, J. LI, J. MENG, D. QIOU, R. SCHMIDT, M. TRICK & J. WILMER Establishment of an Integrated Marker System for Oilseed Rape Breeding (IMSORB). Acta Hort. (ISHS) 706: 195-202.
2006 LYSAK, M.A., A. BERR, A. PECINKA, R. SCHMIDT, K. MCBREEN & I. SCHUBERT Mechanisms of chromosome number reduction in Arabidopsis thaliana and related Brassicaceae species. Proc. Natl. Acad. Sci. USA 103: 5224-5229.
2006 QIU, D. C. MORGAN, J. SHI, Y. LONG, J. LIU, R. LI, X. ZHUANG, Y. WANG, X. TAN, E. DIETRICH, T. WEIHMANN, C. EVERETT, S. VANSTRAELEN, P. BECKETT, F. FRASER, M. TRICK, S. BARNES, J. WILMER, R. SCHMIDT, J. LI, J. MENG & I. BANCROFT A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor. Appl. Genet. 114: 67-80.
2006 SCHMIDT, R. Comparative structural genome analysis – transfer of data across species. In: European Training and Networking Activity. Plant Genomics and Bioinformatics Expression Micro Arrays and Beyond – a course book (ed. J. Freitag), MPI-MPP 2006, Potsdam-Golm, pp. 16-29.
2006 SCHMIDT, R. & M. ARLT Gene-Silencing in transgenen Pflanzen. Max-Planck-Gesellschaft, Jahrbuch, Tätigkeitsberichte 2006. MPI für Molekulare Pflanzenphysiologie, Postdam.
[ ^ ] 2005
2005 DETTMER, J., D. SCHUBERT, O. CALVO-WEIMAR, Y.-D. STIERHOF, R. SCHMIDT & K. SCHUMACHER Essential role of the V-ATPase in male gametophyte development. Plant J. 41: 117-124.
2005 GEBHARDT, C., R. SCHMIDT & K. SCHNEIDER Plant genome analysis: the state of the art. Int. Rev. Cytol. 247: 223-284.
[ ^ ] 2004
2004 BOIVIN, K., A. ACARKAN, R.S. MBULU, O. CLARENZ & R. SCHMIDT The Arabidopsis genome sequence as a tool for genome analysis in Brassicaceae. A comparison of the Arabidopsis and Capsella rubella genomes. Plant Physiol. 135: 735-744.
2004 SCHUBERT, D., B. LECHTENBERG, A. FORSBACH, M. GILS, S. BAHADUR & R. SCHMIDT Silencing in Arabidopsis T-DNA transformants – the predominant role of a gene-specific RNA sensing mechanism versus position effects. Plant Cell 16: 2561-2572.
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Staff
scientific staff
Boudichevskaia, Anastassia +49 (0)39482 5344
Cao, Dr. Xuan Hieu +49 (0)39482 5260
Koppolu, Jahnavi +49 (0)39482 5765
Le, Thanh Loan +49 (0)39482 5260
Schmidt, Dr. Renate +49 (0)39482 5591
staff or visitors
Dung, Le Phuong +49 (0)39482 5765
Fabig, Mandy
Herz, Kathleen
Langanke, Kristin +49 (0)39482 5765
Walter, Christa +49 (0)39482 5684
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