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The impact of duplications in the oilseed rape genome
Polyploidy is widespread in the plant kingdom. We are therefore interested to trace the fate of duplicated regions in the course of evolution. We use the allopolyploid species Brassica napus (oilseed rape) to study the arrangement, structure and expression of duplicated genes in selected regions of the rapeseed genome. A PCR-based screening platform of an oilseed rape BAC library enables quick access to regions of interest in the rapeseed genome. Furthermore, we determine the allelic diversity of selected genes in a broad set of oilseed rape accessions.
Genetic analysis of seed traits in Arabidopsis thaliana
We use the natural occurring variation in Arabidopsis thaliana accessions to study the genetic factors of importance for the trait seed yield. We determine characters such as seed weight, seed size, seed number per silique and seed yield per plant in a population of recombinant inbred lines and in a broad set of Arabidopsis thaliana accessions. QTL mapping and association studies are used to identify loci of importance for these traits.
Transgene silencing in Arabidopsis thaliana
Silencing of introduced genes is often observed in transgenic plants. A systematic analysis of transgene expression in Arabidopsis thaliana showed that transgene silencing is triggered by excessive transcript levels. The transgene expression level correlates with the probability that silencing is initiated early in plant development. The study of progeny of plants with silenced transgenes showed that the silenced state is not transmitted to the next generation, instead silencing has to be established in each generation anew. We are studying now how different environmental factors and genetic backgrounds affect the onset and/or the spread of silencing.
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| 2011 |
SCHMIDT, R. & I. BANCROFT (EDS.)
| Genetics and Genomics of the Brassicaceae. Plant Genetics and Genomics: Crops and Models, Vol. 9. Springer-Verlag New York Dordrecht Heidelberg London. |
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| 2011 |
SCHMIDT, R. & I. BANCROFT
| Perspectives on genetics and genomics of the Brassicaceae. In: SCHMIDT, R. & I. BANCROFT (Eds.): Genetics and Genomics of the Brassicaceae. Springer-Verlag New York Dordrecht Heidelberg London, pp. 617-632. |
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| 2011 |
TOWN, C., R. SCHMIDT & I. BANCROFT
| Comparative genome analysis at the sequence level in the Brassicaceae. In: SCHMIDT, R. & I. BANCROFT (Eds.): Genetics and Genomics of the Brassicaceae. Springer-Verlag New York Dordrecht Heidelberg London, 171-194. |
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| 2008 |
FISCHER, U., M. KUHLMANN, A. PECINKA, R. SCHMIDT & M.F. METTE
| Local DNA features affect RNA-directed transcriptional gene silencing and DNA methylation. Plant J. 53: 1-10. |
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| 2007 |
ECKE W., M. LANGE, R. SCHMIDT, K. BACH, E. DIETRICH*, R. SNOWDON, K. LINK, M. OUZUNOVA, F. BREUER, D. STELLING, D. HAUSKA, G. LECKBAND, F. DREYER, J. DETERING, P. DUSCHERER, W. HORN & S. TUVESSON
| GABI-Bridge: Die Brücke von Sequenz zu Ölgehalt und Resistenz: DNA-Unterschiede kontrollieren agronomisch wichtige Merkmale bei Raps. In: Die Highlights aus der zweiten GABI Förderperiode. GenomXPress Sonderausgabe 1/07, 32. |
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| 2007 |
NASRALLAH, J.B., P. LIU, S. SHERMAN-BROYLES, R. SCHMIDT & M.E. NASRALLAH
| Epigenetic mechanisms for breakdown of self-incompatibility in inter-specific hybrids. Genetics 175: 1965-1973. |
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| 2006 |
BANCROFT, I., S. BARNES, J. LI, J. MENG, D. QIOU, R. SCHMIDT, M. TRICK & J. WILMER
| Establishment of an Integrated Marker System for Oilseed Rape Breeding (IMSORB). Acta Hort. (ISHS) 706: 195-202. |
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| 2006 |
LYSAK, M.A., A. BERR, A. PECINKA, R. SCHMIDT, K. MCBREEN & I. SCHUBERT
| Mechanisms of chromosome number reduction in Arabidopsis thaliana and related Brassicaceae species. Proc. Natl. Acad. Sci. USA 103: 5224-5229. |
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| 2006 |
QIU, D. C. MORGAN, J. SHI, Y. LONG, J. LIU, R. LI, X. ZHUANG, Y. WANG, X. TAN, E. DIETRICH, T. WEIHMANN, C. EVERETT, S. VANSTRAELEN, P. BECKETT, F. FRASER, M. TRICK, S. BARNES, J. WILMER, R. SCHMIDT, J. LI, J. MENG & I. BANCROFT
| A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor. Appl. Genet. 114: 67-80. |
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| 2006 |
SCHMIDT, R.
| Comparative structural genome analysis – transfer of data across species. In: European Training and Networking Activity. Plant Genomics and Bioinformatics Expression Micro Arrays and Beyond – a course book (ed. J. Freitag), MPI-MPP 2006, Potsdam-Golm, pp. 16-29. |
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| 2006 |
SCHMIDT, R. & M. ARLT
| Gene-Silencing in transgenen Pflanzen. Max-Planck-Gesellschaft, Jahrbuch, Tätigkeitsberichte 2006. MPI für Molekulare Pflanzenphysiologie, Postdam. |
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| 2005 |
DETTMER, J., D. SCHUBERT, O. CALVO-WEIMAR, Y.-D. STIERHOF, R. SCHMIDT & K. SCHUMACHER
| Essential role of the V-ATPase in male gametophyte development. Plant J. 41: 117-124. |
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| 2005 |
GEBHARDT, C., R. SCHMIDT & K. SCHNEIDER
| Plant genome analysis: the state of the art. Int. Rev. Cytol. 247: 223-284. |
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| 2004 |
BOIVIN, K., A. ACARKAN, R.S. MBULU, O. CLARENZ & R. SCHMIDT
| The Arabidopsis genome sequence as a tool for genome analysis in Brassicaceae. A comparison of the Arabidopsis and Capsella rubella genomes. Plant Physiol. 135: 735-744. |
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| 2004 |
SCHUBERT, D., B. LECHTENBERG, A. FORSBACH, M. GILS, S. BAHADUR & R. SCHMIDT
| Silencing in Arabidopsis T-DNA transformants – the predominant role of a gene-specific RNA sensing mechanism versus position effects. Plant Cell 16: 2561-2572. |
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