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The Applied Biochemistry group aims at the identification of mechanisms regulating plant secondary metabolism. For these studies, a range of model and crop plants are used. Secondary metabolites significantly contribute to the interaction of plants with their environment, e.g. by acting as defence compounds. Plants synthesize a plethora of secondary compounds in response to stresses, and the accumulated substances can show anti-microbial properties, contribute to protection against UV light and act as antioxidants or signalling molecules. The role of secondary compounds as part of the human nutrition is another important aspect.
To identify regulatory factors which control the allocation of resources into different branches of secondary metabolism, the research group particularly uses proteome approaches and metabolite profiling. The functions of specific metabolites and proteins in the defence of abiotic or biotic stresses are investigated by using transgenic plants and mutants. As a model system, tobacco trichomes are studied using molecular and biochemical techniques and methods of cell biology; trichomes with glandular function able to secrete secondary compounds are of particular interest.
Furthermore, the cellular responses of plants upon cold stress are analysed by metabolite analysis and proteome approaches. Techniques for metabolite profiling are also used to characterize accessions from the IPK seed collection. In cooperation with external partners, the potential beneficial health effects of nutritional flavonoids are investigated.
The group has introduced techniques of proteome analysis as part of a functional genomics platform. The repertoire of methods comprises the separation of complex protein extracts by 2-D gel electrophoresis and LC-based techniques and the identification of relevant proteins by mass spectrometry using MALDI-TOF MS and ESI-Q-TOF MS/MS. In a range of projects, proteome approaches are used to characterize the protein complement of different plant tissues, including the seed proteome of barley and studies on the salt tolerance of barley accessions. An overall aim is to elucidate regulatory networks in plant stress defence on the protein level with particular emphasis on post-translational modifications, e.g. phosphorylation and on the composition and function of protein complexes in defence responses. Data analysis is performed in close collaboration with bio-informatics groups.
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| 2011 |
BAZARGANI, M.M., E. SARHADI; A.-A BUSHEHRI, A. MATROS, H.-P. MOCK, M.-R. NAGHAVI, V. HAJIHOSEINI, M. MARDI, M.-R. HAJIREZAEI, F. MORADI; B. EHDAIE & G.H. SALEKDEH
| A proteomics view on the role of drought-induced senescence and oxidative stress defense in enhanced stem reserves remobilization in wheat. journal of Preteomics: doi:101016/j.jprot.2011.05.015. |
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| 2011 |
KASPAR, S., M. PEUKERT, A. SVATOS, A. MATROS & H.P. MOCK
| MALDI-imaging mass spectrometry - An emerging technique in plant biology. Proteomics 11 (2011) 1840-1850. |
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| 2011 |
MATROS, A., S. KASPAR, S: TENZER, M: KIPPING; U. SEIFFERT & H.P. MOCK
| Label-free liquid chromatography-based quantitative proteomics: challenges and recent developments. In: RANCOURT, G.C (Ed.): Proteomics: Methods, applications and limitations (Protein biochemistry, synthesis structure and cellular funtions). Nova Science Publishers Inc., New York. 103 - 136. |
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| 2011 |
MATROS, A., S. KASPAR; K: WITZEL & H.P. MOCK
| Recent progress in liquid chromatography-based separation and label-free quatitative plant proteomics. Phytochemistry 72: 963-974. |
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| 2011 |
MOLLER, AL.B., K. WITZEL, A. VERTOMMEN, V. BARKHOLT, B. SVENNSON; S: CARPENTIER; H.-P. MOCK & C. FINNIE
| Plant plasma membrane proteomics: challenges and possiblilities. In: IVANOV, A.R. & A.V. LAZAREV (Eds.): Sample preparation in biological mass spectrometry. Springer, Dordrecht 411-434. |
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| 2011 |
WITZEL, K., C: PIETSCH; M: STRICKERT; A. MATROS, M. RÖDER, W: WESCHKE; U. WOBUS & H.P. MOCK
| Mapping of quantitative trait loci associated with protein expression variation in barley grains. Mo. Breed. 27: 201-314. |
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| 2011 |
MATROS, A., S. KASPAR, S. TENZER, M: KIPPING, U. SEIFFERT & H.P. MOCK
| Label-free liquid chromatorgraphy-based quantitative proteomics: challenges and recent developments. In: RANCOURT, G.C. (Ed): Proteomics: Methods, applications and limitations (Protein biochemistry, synthesis, struture and cellular functions). Nova Science Publishers Inc. New York. 103-136. |
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| 2011 |
MOLLER, A.L.B., K. WITZEL, A. VERTOMMEN; V. BARKHOLT, B: SVENNSON, S. CARPENTIER; H.-P. MOCK & C. FINNIE
| Plant plasma membrane proteomics: challenges and possibilities. In: IVANOV A.R. & A.V. LAZAREV (Eds.): Sample preparation in biological mass spectrometry. Springer, Dordrecht. 411-434. |
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| 2010 |
AGARWAL, R., A. MATROS, M. MELZER, H.-P. MOCK & J.K. SAINIS
| Heterogeneity in thylakoid membrane proteome of Synechocystis 6803. J. Proteomics 73(5): 976-991. |
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| 2010 |
SREENIVASULU, N., L. BORISJUK, B.H. JUNKER, H.-P. MOCK, H. ROLLETSCHEK, U. SEIFFERT, W. WESCHKE & U. WOBUS
| Barley grain development toward an integrative view. Int. Rev. Cell. Mol. Biol. 281: 49-89. |
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| 2010 |
KASPAR, S., A. MATROS & H.-P. MOCK
| Proteome and flavonoid analysis reveals distinct responses of epidermal tissue and whole leaves upon UV-B radiation of barley (Hordeum vulgare L.) seedlings. J. Proteome Res. 9(5): 2402-2411. |
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| 2010 |
WITZEL, K., A. WEIDNER, G.K. SURABHI, R.K. VARSHNEY, G. KUNZE, G.H. BUCK-SORLIN, A. BOERNER & H.-P. MOCK
| Comparative analysis of the grain proteome fraction in barley genotypes with contrasting salinity tolerance during germination. Plant Cell Environ. 33(2): 211-222. |
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| 2010 |
MATROS, A., S. KASPAR, K. WITZEL & H.-P. MOCK
| Recent progress in liquid chromatography-based separation and label-free quantitative plant proteomics. |
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| 2010 |
KASPAR, S., D. WEIER, W. WESCHKE, H.-P. MOCK & A. MATROS
| Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains. |
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| 2010 |
WITZEL, K., C. PIETSCH, M: STRICKERT; A: MATROS, M. RÖDER, W. WESCHKE, U. WOBUS & H. P. MOCK
| Mapping of quantitative trait loci associated with protein expression vairation in barley grains. Mol. Breed. 27 (2010) 301-314. |
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| 2010 |
SEIFERT, U., F. BOLLENBECK, H.P. MOCK & A. MATROS
| Clustering of crop phenotypes by means of hyperspectral signatures using artificial neural networks. Proceedings of the 2nd IEEE Workshop on Hyperspectral Imaging and Signal Processing: Evolution in Remote Sensing WHISPERS 2010, Reykjavik/ Iceland. IEE Press 31-34. |
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| 2009 |
LIPPMANN, R. S. KASPAR, T. RUTTEN, M. MELZER, J. KUHMLEHN, A. MATROA & H.-P. MOCK
| Protein and metabolite analysis reveals permanent induction of stress defense and cell regeneration processes in a tobacco cell suspension culture. Int. J. Mol. Sci. 10(7): 3012-32.
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| 2009 |
WITZEL, K., A. WEIDNER, G.K. SURABHI, A. BÖRNER & H.-P. MOCK
| Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity. J. Exp. Bot. 60(12): 3545-57. |
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| 2009 |
BÖER, E., R. BODE, H.-P. MOCK, M. PIONTEK & G. KUNZE
| Atan1p-an extracellular tannase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme. Yeast 26(6): 323-37. |
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| 2009 |
JAHN, D., A. MATROS,A.Y. BAKULINA, J. TIEDEMANN, U. SCHUBERT, M. GIERSBERG, S. HAEHNEL, K. ZOUFAL, H.-P. MOCK & S.M. KIPRIYANOV
| Model structure of the immunodominant surface antigen of Eimeria tenella identified as a target for sporozoite-neutralizing monoclonal antibody. Parasitol. Res. 105(3): 655-68. |
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| 2009 |
RADCHUK, V.V., L. BORISJUK, N. SREENIVASULU, K. MERX, H.-P. MOCK, H. ROLLETSCHEK, U. WOBUS & W. WESCHKE
| Spatiotemporal profiling of starch biosynthesis and degradation in the developing barley grain. Plant Physiol. 150(1): 190-204. |
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| 2008 |
BÖTTCHER, C., D. CENTENO, J. FREITAG, R. HÖFGEN, K. KÖHL, J. KOPKA, J. KROYMANN, A. MATROS, H.P. MOCK, S. NEUMANN, M. PFALZ, E. VON ROEPENACK-LAHAYE, N. SCHAUER, S. TRENKAMP, M. ZUBRIGGEN & A.R. FERNIE
| Teaching (and learning from) metabolomics: the 2006 PlantMetaNet ETNA Metabolomics Research School. Physiol. Plant. 132 (2): 136-149. |
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| 2008 |
BRUMBAROVA, T., A. MATROS, H.-P. MOCK & P. BAUER
| A proteomic study showing differential regulation of stress, redox regulation and peroxidase proteins by iron supply and the transcription factor FER. Plant J. 54: 321-334. |
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| 2008 |
MATROS, A. & H.-P. MOCK
| Yeast proteome analysis. In: Satyanarayana, T. & G. Kunze (Eds.): Diversity and potential biotechnological applications in yeasts. Springer Verlag, Berlin – Heidelberg – New York, in press |
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| 2008 |
IVANOV, R., J. TIEDEMANN, A. CZIHAL, A. SCHALLAU, L. DIEP, H.-P. MOCK, B. CLAUS, A. TEWES & H. BÄUMLEIN
| EFFECTOR OF TRANSCRIPTION2 is involved in xylem differentiation and includes a functional DNA single strand cutting domain. Dev. Biol. 313: 93-106. |
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| 2008 |
KORN, M., S. PETEREK, H.P. MOCK, A.G. HEYER & D.K. HINCHA
| Heterosis in the freezing tolerance, and sugar and flavonoid contents of crosses between Arabidopsis thaliana accessions of widely varying freezing tolerance. Plant Cell Environ. 31: 813-827. |
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| 2008 |
TIEDEMANN, J., T. RUTTEN, G. MÖNKE, A. VORWIEGER, H. ROLLETSCHEK, D. MEISSNER, C. MILKOWSKI, S. PETERECK, H.-P. MOCK, T. ZANK & H. BÄUMLEIN
| Dissection of a complex seed phenotype: novel insights of FUSCA3 regulated developmental processes. Dev. Biol. 317: 1-12. |
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| 2008 |
TOUFEKTSIAN, M.C., M. LORGERIL, N. NAGY, P. SALEN, M.B. DONATI, L. GIORDANO, H.-P. MOCK, S. PETEREK, A. MATROS, K. PETRONI, R. PILU, D. ROTILIO, C. TONELLI, J. DE LEIRIS, F. BOUCHER & C. MARTINS
| Chronic dietary intake of plant-derived anthocyanins protects the rat heart against ischemia-reperfusion injury. J. Nutr. 138 (4): 747-752. |
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| 2008 |
STRICKERT, M., K. WITZEL, J. KEILWAGEN, H.-P. MOCK, P. SCHNEIDER, M. BIEHL & T. VILLMANN
| Adaptive matrix metrics for attribute dependence analysis in differential high-throughput data. 5th International Workshop on Computational Systems Biology (WCSB), Leipzig. 190-193. |
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| 2008 |
MOCK, H.-P. & A. MATROS
| Proteome analysis of cellular responses to abiotic stresses in plants. In: AGRAWAL, G.K. & RAKWAL, R. (Eds.): Plant Proteomics: Technologies, Strategies, and Applications. Wiley, Hoboken, New Jersey, pp. 605-628. |
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| 2008 |
BUTELLI, E., L. TITTA, M. GIORGIO, H.P. MOCK, A. MATROS, S. PETEREK, E. SCHIJLEN, R.D. HALL, A.G. BOVY, J. LUO & C. MARTIN
| Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nature Biotechnology 26: 1301-1308. |
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| 2007 |
MOCK, H.-P.
| Tocopherol composition of plants and their regulation. In: PREEDY, V. & R.R. WATSON (Eds.): The Encyclopedia of Vitamin E. CABI Publishing, Wallingford/UK, 112-121. |
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| 2007 |
BARTELS, A., H.P. MOCK & J. PAPENBROCK
| Differential expression of Arabidopsis sulfurtransferases under various growth conditions. Plant Physiology and Biochemistry 45: 178-187. |
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| 2007 |
GIGOLASHVILI, T., B. BERGER, H.P. MOCK, C. MÜLLER, B. WEISSHAAR & U.I. FLUEGGE
| The transcription factor HIG1/MYB51 regulates indolic glucosinolate biosynthesis in Arabidopsis thaliana. Plant Journal 50: 886-901. |
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| 2007 |
VORWIEGER, A., C. GRYCZKA, A. CZIHAL, D. DOUCHKOV, J. TIEDEMANN, H.P. MOCK, M. JAKOBY, B. WEISSHAAR, I. SAALBACH & H. BÄUMLEIN
| Iron assimilation and transcription factor controlled synthesis of riboflavin in plants. Planta 226: 147-158. |
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| 2007 |
WITZEL, K, G.K. SURABHI, G. JYOTHSNAKUMARI, C. SUDHAKAR, A. MATROS & H.P. MOCK
| Quantitative proteome analysis of barley seeds using ruthenium(II)-tris-(bathophenanthroline-disulphonate) staining. Journal of Proteome Research 6: 1325-1333. |
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| 2007 |
BÖER, E., G. STEINBORN, A. MATROS, H.-P. MOCK, G. GELLISSEN & G. KUNZE
| Production of interleukin-6 in Arxula adeninivorans, Hansenula polymorpha and Saccharomyces cerevisiae applying the wide range yeast vector (CoMedTM) system to simultaneous comparative assessment. FEMS Yeast Res. 7: 1181-1187. |
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| 2006 |
AMME, S., A. MATROS, B. SCHLESIER & H.P. MOCK
| Proteome analysis of cold stress response in Arabidopsis thaliana using DIGE-technology. Journal of Experimental Botany 57: 1537-1546. |
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| 2006 |
MATROS, A., S. AMME, B. KETTIG, G.H. BUCK-SORLIN, U. SONNEWALD & H.P. MOCK
| Growth at elevated CO2 concentrations leads to modified profiles of secondary metabolites in tobacco cv. SamsunNN and to increased resistance against infection with potato virus Y. Plant Cell and Environment 29: 126-137. |
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| 2006 |
ROSSIGNOL, M, J.B. PELTIER, H.P. MOCK, A. MATROS, A.M. MALDONADO & J.V. JORRIN
| Plant proteome analysis: A 2004-2006 update. Proteomics 6: 5529-5548. |
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| 2006 |
STRICKERT, M., T. CZAUDERNA, S. PETEREK, A. MATROS, H.-P. MOCK & U. SEIFFERT
| Full length HPLC signal clustering and biomarker identification in tomato plants. In: Ruan, D., P. D'hondt, P.F. Fantoni, M. De Cock, M. Nachtegael & E.E. Kerre (Eds.): Applied Artificial Intelligence. World Scientific, Singapore, 549-556. ISBN 981-256-690-2. |
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| 2006 |
STRICKERT, M, N. SREENIVASULU, S. PETEREK, W. WESCHKE, H.P. MOCK & U. SEIFFERT
| Unsupervised feature selection for biomarker identification in chromatography and gene expression data. In Artificial Neural Networks in Pattern Recognition, Proceedings, 4087: 274-285. |
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| 2006 |
ZIMMERMANN, G., H. BÄUMLEIN, H.P. MOCK, A. HIMMELBACH & P. SCHWEIZER
| The multigene family encoding germin-like proteins of barley. Regulation and function in basal host resistance. Plant Physiology 142: 181-192. |
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| 2006 |
SCHLESIER, B. & H.-P. MOCK
| Protein isolation and 2-D electrophoretic separation. In: Salinas, J. & J.J. Sanchez-Serrano (Eds.): Arabidopsis protocols, 2nd ed. Humana Press, Totowa/USA, 381-391. |
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| 2006 |
ELLERSTRÖM, M., W. REIDT, R. IVANOV, J. TIEDEMANN, M. MELZER, A. TEWES, T. MORITZ, H.-P. MOCK, F. SITBON, L. RASK & H. BÄUMLEIN
| Ectopic expression of effector of transcription perturbs gibberellin-mediated plant developmental processes. Plant Molecular Biology 59(4): 663-681. |
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| 2006 |
SCHLESIER, B. & H.-P. MOCK
| Protein isolation and 2-D electrophoretic separation. In: SANCHEZ-SERRANO, J. & J. SALINAS (Eds.): Arabidopsis protocols. (2nd). Humana Press, Totowa/USA. Methods Mol. Biol. 323: 381-391. |
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| 2005 |
AMME, S., T. RUTTEN, M. MELZER, G. SONSMANN, J.P.C. VISSERS, B. SCHLESIER & H.-P. MOCK
| A proteome approach defines protective functions of tobacco leaf trichomes. Proteomics 5: 2508-2518. |
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| 2005 |
BÖER, E., H.-P. MOCK, R. BODE, G. GELLISSEN & G. KUNZE
| An extracellular lipase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ALIPI gene and characterization of the purified recombinant enzyme. Yeast 22: 523-535. |
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| 2005 |
SCHNEIDER, A., T. KIRCH, T. GIGOLASHVILI, H.-P. MOCK, U. SONNEWALD, R. SIMON, U.I. FLÜGGE & W. WERR
| A Transposon Based Activation Tagging Population in Arabidopsis thaliana (TAMARA) useful for the identification of developmental and metabolic mutants. FEBS Lett. 579: 4622-4628. |
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| 2005 |
SPENCER, J.P.E., G.C. KUHNLE, M. HAJIREZAEI, H.-P. MOCK, U. SONNEWALD & C. RICE-EVANS
| The genotypic variation of the antioxidant potential of different tomato varieties. Free Radical Research 39: 1005-1016. |
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| 2004 |
CANOVAS, F.M., E. DUMAS-GAUDOT, G. RECORBET, J. JORRIN, H.-P. MOCK & M. ROSSIGNOL
| Plant proteome analysis. Proteomics 4: 285-298. |
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| 2004 |
FENG, C, A. ANDREASSON, A. MASLAK, H.-P. MOCK, O. MATTSON & J. MUNDY
| Arabidopsis MYB68 in development and responses to environmental cues. Plant Science 167: 1099-1107. |
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| 2004 |
MATROS, A. & H.-P. MOCK
| Ectopic expression of a UDP-Glucose: phenylpropanoid glucosyltransferase leads to increased resistance of tobacco plants against infection with PVY. Plant Cell Physiol. 45: 1185-1193. |
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| 2004 |
MÖNKE, G., L. ALTSCHMIED, A. TEWES, W. REIDT, H.-P. MOCK, H. BÄUMLEIN & U. CONRAD
| Description of the molecular interaction of seed specific transcription factors FUS3 and ABI3 with their corresponding DNA target. Planta 219: 158-166. |
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| 2004 |
SCHLESIER, B., A. BERNA, F. BERNIER & H.-P. MOCK
| Proteome analysis differentiates between two highly homologues germin-like proteins in Arabidopsis thaliana ecotypes Col-0 and Ws-2. Phytochemistry 65: 1565-1574. |
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secondary plant metabolism, stress defense, proteomics, functional analysis of plants
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