Five BAC libraries of cultivar 'Morex' are available for the construction of a High Information Content Fingerprint (HICF) map of the barley genome
BAC-librarya
Cultivar
# of clones
ř insert size
Genome coverage
Restriction enzyme
1 HVVMRXALLhAb
Morex
313,344
106 kb
6.6
Hind III
2 HVVMRXALLhBc
Morex
268,800
105 kb
6.0
Hind III
3 HVVMRXALLeAc
Morex
147,456
125 kb
3.7
Eco RI
4 HVVMRXALLmAd
Morex
202,752
147 kb
6.0
Mbo I
5 HVVMRXALLrAd
Morex
253,440
92 kb
4.6
Random sheared
anaming follows an agreed general nomenclature for Triticeae BAC libraries: 3 letters species code, 3 letters cultivar code, three letter genome representation (i.e. ALL= whole genome, 1HS = short arm chromosome 1H), 1 letter enzyme, 1 letter library identifier in case of multiple libraries per enzyme; bYu et al., 2000; cLangridge et al., unpublished data; dStein et al. unpublished data
Copies of libraries, clone handling and screening of libraries is available for libraries 1 + 3 from Clemson University Genomics Institute (CUGI) and for libraries 2,4,5 from Centre National des Ressources Génomiques Végétales (CNRGV).
