Super Resolution Microscopy

Super Resolution Microscope System Elyra PS.1 

 

We use a Super Resolution Fluorescence Microscope Elyra PS.1 ([link]https://www.zeiss.com/microscopy/int/products/superresolution-microscopy.html) allowing the duplication of conventional fluorescence microscopy resolution until ca. 100 nm by applying Structured Illumination Microscopy (SIM). In addition, with PALM (Photoactivated Localization Microscopy) an extra resolution up to 20 nm laterally and up to 100 nm axially can be realized. Thus smallest fluorescence labelled cell structures can be visualized until now only non-selectively detectable by electron microscopy. Hence, for the first time multiple labelled structures can be analysed at a very high resolution.

Applying these techniques a new quality of chromatin structure research may be reached. Chromatin structures not detectable till now will become differentially visible in fixed tissues as well as in in vivo

After agreement, the new microscope is applicable for all IPK research projects and external collaborators that require a very high resolution.

For additional information please contact Veit Schubert ([link]schubertv(at)ipk-gatersleben.de).

Barley cytokinesis captured by SIM using the Elyra microscope in comparison to conventional fluorescence widefield microscopy.

SIM allows to identify structures till ca. 100nm labelled by rhodamine (tubulin) and DAPI (general chromatin-labelling) not visible applying conventional microscopy.

 

The distribution of the condensin CAP-D3 (Alexa488) and cohesin SMC3 (rhodamin) subunits in an Arabidopsis nucleus acquired for comparison by SIM and widefield microscopy.