Super Resolution Microscopy
We use a Super Resolution Fluorescence Microscope Elyra PS.1 (https://www.zeiss.com/microscopy/int/products/superresolution-microscopy.html) allowing the duplication of conventional fluorescence microscopy resolution until ca. 100 nm by applying Structured Illumination Microscopy (SIM). In addition, with PALM (Photoactivated Localization Microscopy) an extra resolution up to 20 nm laterally and up to 100 nm axially can be realized. Thus smallest fluorescence labelled cell structures can be visualized until now only non-selectively detectable by electron microscopy. Hence, for the first time multiple labelled structures can be analysed at a very high resolution.
Applying these techniques a new quality of chromatin structure research may be reached. Chromatin structures not detectable till now will become differentially visible in fixed tissues as well as in in vivo.
After agreement, the new microscope is applicable for all IPK research projects and external collaborators that require a very high resolution.
For additional information please contact Veit Schubert (schubertv(at)ipk-gatersleben.de).