Projektporträt „dsRNAguard“Kulturpflanzen schalten die Gene ihrer Feinde ab

auf [link]Pflanzenforschung.de

Neue Perspektiven für den Pflanzenschutz mit HIGS - Interview mit Dr. habil. Patrick Schweizer über das Projekt „dsRNAguard“

auf [link]Pflanzenforschung.de

Methods

For the identification of genes that are relevant to the interaction of the barley powdery mildew fungi and one of wheat with the pathogens of Fusarium, we use various methods of reverse genetic

  • Transcript profiling using arrays (made in house and Agilent 44 k).
  • Single transcript analysis using TaqMan RT-qPCR.
  • "Transient-induced gene silencing" (TIGS) by biolistics in leaf epidermal cells.
  • Transient gene (over)expression by biolistics under the control of their own or of the CaMV 35S promoter.
  • "Virus-induced gene silencing" (VIGS) mediated by the "barley stripe mosaic virus" in barley seedlings and adult plants of wheat.
  • Stable transgenic barley and wheat plants carrying RNAi or (over) expression cassettes for 1-3 genes (cooperation with AG plant reproductive biology).
  • Microscope robotics and automated pattern recognition in microscopic images of powdery mildew interactions.
  • Allele re-sequencing of candidate genes in "trait-customized" barley collections of the IPK gene bank.
  • Fine mapping and backcrosses of potentially valuable alleles (in cooperation with external partners).
  • Protein-protein interaction studies of validated candidates by means of yeast 2-hybrid and other systems.