Wheat pollen viability (2016 - 2019)
Wheat pollen are classified as short living that die within short periods after shedding. Due to little information available, the project carries out physiological, biochemical and genetic mechanism of wheat pollen viability. In close cooperation with the ‘Applied Biochemistry’ and ‘Quantitative Genetic’ working group at IPK metabolic profiles and quantitative genetic methods should predict pollen viability and test appropriate storage methods. The gained know-how may provide a breeders tool for crossing management.
KALT Project - Cryostress - mechanisms of cellular adaptation to extremely low temperatures (2013 - 2017)
A significant portion of the biosphere exists in the frozen state but still harbors viable and physiologically active microorganisms. The project focuses on so-far unknown key physiological responses towards cryostress and their molecular basis. Selected bacteria, protozoa, fungi as well as animal and plant cell lines are investigated in a comparative approach.
Main topics are changes in the permeability of the cell envelope, in energy metabolism and cell proliferation. Genes involved in the response to cryostress are analyzed by transcriptomics, proteomics and methylomics approaches. A final goal of the study is the analysis of novel cryoprotectants and their mode of action.
- Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie-Hans-Knöll-Institut (HKI)
- Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
- Ludwig-Maximilians-Universität München (LMU)
- Experimentelle Phykologie und Sammlung von Algenkulturen der Universität Göttingen (SAG)
- Universitätsmedizin der Georg-August-Universität
DAAD project on garlic cryopreservation (2014 - 2018)
The Gatersleben genebank comprises one of the largest garlic cryo-collections, which include 338 field and 122 cryo accessions. Evaluation of abiotic stress conditions including dehydration, hypoxia and cold are prevalent in garlic cryopreservation. During the DAAD-project, the metabolite profile and transcriptome of two contrasting cryo-storable garlic accessions are investigated. The gene expression among susceptible and tolerant garlic genotypes are analysed and are supposed to highlight differential cryo-stress tolerances and enhance our understanding on cryo-stress conditions.
The EURALLIVEG Project was an international project, which aimed to establish a garlic cryobank in the German, Polish and Czech genebanks. The cryobank comprises about 220 garlic accession and a shallot core collection. The additional image database provides information of 395 accessions including important traits, ontogenesis of plants and intraspecific groups.