Barley Genome Sequencing

The group was leading and coordinating the effort of the [link]International Barley Sequencing Consortium towards complete [link]sequencing of the barley genome. After whole genome physical map construction the seven barley chromosomes were sequenced on the basis of a non-redundant [link]minimum tiling path of overlapping BAC clones. The reference sequence of barley was recently published in the journal [link]Nature. The assembly data can be accessed [link]here.

Barley genetic diversity

In the [link]BRIDGE project funded by the Leibniz Association our group is working in collaboration with several partners at IPK on an effort of genotyping all barley accessions (>20,000) of IPK`s ex situ genebank. The data is a first systematic effort of capturing global genome diversity of a barley world collection of genetic resources and the data is being used for GWAS and population genetic analyses. The data will be hosted in a project data warehouse together with additional internal and external sources of information on the barley accessions. New front ends and applications are under development to allow more educated access and selection of genetic resources by researchers and breeders. The project is aligned to the goals of the[link] DIVSEEK initiative.

Barley Pan-Genome analysis

We started recently the project [link]SHAPE, aiming at unraveling the complexity of the barley Pan-Genome (the sum of (i) the core-genome shared in all barley haplotypes and the (ii) dispensable part of the genome found only individual haplotypes). This includes generating additional reference quality genome assemblies and using different NGS-based approaches to monitor small to large scale structural variation and combine with other publicly available sources of barley genome diversity in a high density variation matrix, which will then be tested for its relevance in barley genomic selection breeding programs.

Barley gene cloning

We use state of the art genomics based approaches of gene cloning in barley. Main focus is on genes controlling virus resistance, plant morphology, organelle development.