Gas chromatography - mass spectrometry (GC-MS, Leco HT TOF-MS)
Using a combination of a LECO HT time-of-flight mass spectrometer, an AGILENT 7890A gas chromatograph equipped with a GERSTEL MPS2XL autosampler, we are able to quantify a large array of polar plant metabolites. Typically, these comprise a number of 60-100 known metabolites like amino acids, organic acids, polyamins, sugars, sugar phosphates etc. and twice the number of metabolites with unknown chemical structure. Routinely we apply in-line derivatization (derivatization of each individual sample prior injection) to improve the quality of our measurements. This enables us to quantify metabolites from large sample numbers in a highly reproducible manner.
High resolution liquid chromatography - mass spectrometry (LC-MS, BRUKER Maxis 2 QTOF-MS)
A high resolution Bruker Maxis 2 quadrupole time-of-flight mass spectrometer (R = 85000, accuracy = 0.6 ppm) hyphenated with an AGILENT 1290 liquid chromatograph (max 1200 bar) and equipped with a GERSTEL autosampler is used for high throughput metabolite profiling of semi-polar compounds like secondary metabolites, phytosterols, vitamins, hormones... and hydrophobic analytes like lipids, tocoperols, pigments... Typically, several Hundreds to Thousands non-redundant chromatographic features (mz) are quantitatively recorded which can be partially annotated by database queries (KNApSAcK, KEGG) using accurate mass, isotopic pattern and MSMS fragment spectra of the analytes. The liquid chromatograph can be coupled to a mikroplate fraction collector (ADVION Nanomate) to comprehensively isolate separated metabolites for in-depth characterization. The analysis time ranges from 5 min (secondary metabolite profiling, lipid profiles of oil storing organs) to 30 min (comprehensive profiling of semi-polar metabolites, lipid profiling of samples with complex lipidomic composition) and allows reproducible measurements of a large number of samples.