Research

Improving agronomically relevant traits in crops or improving biotechnological procedures is the overal aim in own research projects. Thereby our microscopical studies on plants, yeast and bacteria mainly focus on the analysis of cell ultrastructure, the characterization of protein interaction and the visualization of dynamic cellular processes. Often embedded in internal and external collaborations they form an essential supplement to molecular biological and biochemical approaches. Presently we are committed to the following major research projects.

Research projects

 

Identifying molecular triggers for AMMONIUM TRANSPORTER trafficking to target membranes in root cells. 

(DFG funded; Cooperation with Research Group Molecular Plant Nutrition).  [link]more

 

 

The use of genetic diversity and innovative methods for breeding to reduce grain shattering

(BLE funded).  [link]more

 

 

Running cooperations (selected)

 

Internal (IPK)

 

Histological and ultrastructrural examination of transgenic tabacco and Arabidopsis plants exprtessing the cyanobacterial flavodoxin (Fld) (RG Molecular Plant Nutrition);

 

Histological and ultrastructrural examination of Salicornia in regard of salt tolerance (RG Molecular Plant Nutrition);

 

Cell biological studies of the cellular and subcellular spatial and temporal distribution of plant metabolites (RG Applied Biochemistry);

 

Immunogoldlocalisation of Gallus acid decarboxylase and Catechol-1, 2-Dioyxgenase to evaluate functional aspects of the degredation of tannic acid and protocatechuate in Arxula adeninivorans (RG Yeast Genetics);

 

Morphological and histological determination of structural changes of rapeseed root and stem tissue due to boron deficiency (RG Metalloid-Transport);

 

Development and differentiation of the vascular architecture of the barley spike (RG Plant Architecture, RG Assimilate Allocation and NMR);

 

Morphological and histological analysis of Hypericon glands accumulationg hypericin (RG Assimilate Allocation and NMR); Fluoresecence based studies on cellular distribution and mobility of CENH3 associated proteins. (RG Chromosome Structure and Function);

 

Ultrastructrural characterization of B-chromosome positive micronuclei of embryogenic root tissue of Aegilops speltoides (RG Chromosome Structure and Function);

 

Ultrastructrural characterization of nuclei of Chionographis japonica and C. hisauchiana (AG Chromosomenstruktur und -funktion);

 

Structural examination of physiological and biochemical mechanisms of pollen tube development of wheat and other cereals in regard of pollen storage for conservation of genetic resources and improvement of breeding methods (RG Cryo- and Stress Biology);

 

Comparative structural analysis of chlorioplast development in the course of differentiation of meristematic cells of the barley mutant albostrians and mutant of the paralogue gene albostrians-like (RG Genomic of Genetic Ressources);

Structural studies on toothed and smooth barley awns with emphasis on the ploidy of tooth-forming epidermal cells. (RG Genomics of genetic Resources);

 

Histological and ultrastructrural examination of morphological changes in the barley spike during its development for its important role in cereal yield formation. (RG Plant Architecture);

 

Comparative structural analysis of endosperm transfer cells of various barley genotypes (RG Seed Development and RG Plant Architecture);

 

Structural studies on lateral root formation in barley. (RG Seed Development); Comparative ultrastructure of developing embryos of compatible and incompatible wheat x rye crosses. (RG Resources Genetics and Reproduction);

 

External

 

Histological characterization of the lemma of various genotypes of Hordeum vulgare in regard of their threshability (Plant Breeding, Martin-Luther-University of Halle-Wittenberg);

 

Ultrastructural characterization of plasmodesmata (PD) in developing leaves of Arabidopsis WT and mutants of choline transporter-like1 (CHER1) relating to PD genesis and maturation. (Dept. of Biochemistry, University of Erlangen-Nürnberg);

 

Histological determination of chloroplast number/cell in Arabidopsis plants overexpressing the transcription factor GRF5 (Genomics and Proteomics of Plant Systems CEITEC, Masaryk University, Czech Republic);

 

Histological and ultrastructrural examination of phylloid, cortex and medulla of the brown algea Laminaria hyperborea (Division of Glycoscience, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden);

 

Ultrastructrural characterization of plasmodesmata of Populus trichocarpa cell culture (Division of Glycoscience, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden);

 

Electron microscopic characterization of the cellulose synthase rosette at isolated xylem fibres of Populus tremuloides (Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Science, Umeå, Sweden);

 

Comparative ultrastructrural characterization of leaves of WT and transgenic plants of Nicotiana tabacum with expression of a flavodoxin protein from Anabaena in regard of leave senescence (Institute of Molecular and Cellular Biology Rosario, University of Rosario, Argentina);

 

Microscopical characterization of epidermal bladder cells of the model halophyt Mesembryanthemum crystallinum in response to salinity (Southern Cross Plant Science, Southern Cross University, Lismore, Australia and Instituto de Biotecnología, UNAM, Cuernavaca, México);

 

Comparable ultrastructural examination and immunlocalization of transgenic und knock-out plants of Arabidopsis thaliana to characterize the function of a protein involved in the regulation of chloroplast division. (Biochemistry, Max F. Perutz Laboratories University of Vienna, Austria).