Identifying molecular triggers for AMMONIUM TRANSPORTER trafficking to target membranes in root cells 

Marek Marzec

 

The aim of the joint DFG Project with the Molecular Plant Nutrition Group is to uncover new components in the signaling cascade of the N nutritional status in a plant cell. The working concept is rooted on the existence of peptide domains that guide the intracellular membrane localization of ammonium transporter (AMT) proteins in response to changes in the nitrogen nutritional status of the plant and/or the availability of external N. These AMT-specificdomains will be employed to isolate and identify the intracellular signals that ultimately trigger the intracellular relocalization of AMT. Localization studies of AMTs with and without modified peptide domains and the dynamics of AMT redistribution upon external triggers will be analyzed by light and electron microscopy.