The process of bioaccumulation using different sorts of biomass replaces chemical compounds to extract diverse substances, e. g. metals from aqueous liquids. This can be applied to economically exploit poor repositories of raw materials or to remove pollutants from contaminated water supplies. In past projects we have established yeast strains which can accumulate such substances either by uptake into the cell or by sorption to specific binding peptides on the cell wall. The different strains were tested for the accumulation of heavy metals (e. g. copper, nickel), noble metals (e. g. gold, palladium), and rare earth elements (e. g. lanthanum, terbium). The current project aims to apply binding peptides in a cell free system to remove the hazardous substances arsenic and uranium from contaminated ground water.