Infrastructure and Resources

Equipment

Methodologies

  • High-throughput phenotyping
  • high-trhoughput imaging of all commonly determined PSII parameters using kinetic chlorophyll flourescence imaging systems (FlourCam, PSI) integrated into automated phenotyping facilities
  • root phenotyping
  • QTL analyses (association and linkage)
  • Be-sulfite sequencing

Biological Resources

Arabidopsis RIL and IL Populations

5 reciprocal sets of recombinant inbred lines (RILs) were produced by repeated selfing and single-seed descent from 500 F2 plants. The RILs were fully genotyped in the F7, and partially in the F10. [link]Törjek et al. (2006) Theor. Appl. Genet. 113: 1551–1561

 

 

 

2 reciprocal sets of introgression lines (ILs) derived from the combination C24/Col-0 were produced by repeated backcrossing of 200 F2 lines to the maternal parent (receptor). In both sets ((a) 78 ILs with Col-0 background and (b) 62 ILs with C24 background) the donor segments cover ca. 90% of the genome with an average insert size of 18.3 cM. [link](Törjek et al. (2008) J. Hered. 99: 396-406)

Arabidopsis Accessions

The worldwide Arabidopsis population is a diverse collection of genotypes that are adapted to many different environments and therefore display a large variation in many traits, such as morphology, stress tolerance, flowering time and resource efficiency. Three factors guided the assembly of our ‘standard’ Arabidopsis collection of 382 accessions: presence of (public) 250K SNP genotype data, broad range of geographic/climatic/geological origin, low population structure.