Publikationen

Autoren Titel
2015
Hauptmann V Heterologe Expression hochmolekularer repetitiver Spinnenseiden-basierter Proteine in planta sowie deren mechanische und immunologische Charakterisierung. (PhD Thesis, kumulativ) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2015) 75 pp.
Hauptmann V, Menzel M, Weichert N, Reimers K, Spohn U, Conrad U In planta production of ELPylated spidroin-based proteins results in non-cytotoxic biopolymers. BMC Biotechnol. 15 (2015) 9. dx.doi.org/10.1186/s12896-015-0123-2
Hensel G, Floss D M, Arcalis E, Sack M, Melnik S, Altmann F, Rutten T, Kumlehn J, Stoger E, Conrad U Transgenic production of an anti HIV antibody in the barley endosperm. PLoS One 10 (2015) e0140476. dx.doi.org/10.1371/journal.pone.0140476
Klein B Funktionelle Charakterisierung von F-Box-Fusionsproteinen aus Pflanzen. (Bachelor Thesis) Hannover, Leibniz-Universität Hannover, Studiengang Pflanzenbiotechnologie (2015) 47 pp.
Schinköthe A Produktion, Reinigung und Analyse von oligomeren Vogelgrippeantigenen in Pflanzen. (Bachelor Thesis) Jena, Ernst-Abbe-Hochschule Jena, Fachbereich Medizintechnik/Biotechnologie, Studiengang Biotechnologie (2015) 38 pp.
2014
Heigl F Charakterisierung von rekombinanten Antikörpern gegen Chromatinproteine. (Bachelor Thesis) Universität Regensburg (2014) 58 pp.
Koch F Isolation, Modifizierung und Charakterisierung von nanobodies gegen Interleukin-12. (Master Thesis) Jena, Friedrich-Schiller-Universität Jena, Biologisch-Pharmazeutische Fakultät (2014) 47 pp.
Kolotilin I, Topp E, Cox E, Devriendt B, Conrad U, Joensuu J, Stöger E, Warzecha H, McAllister T, Potter A, McLean M D, Hall J C, Menassa R Plant-based solutions for veterinary immunotherapeutics and prophylactics. Vet. Res. 45 (2014) 375 Highly accessed. dx.doi.org/10.1186/s13567-014-0117-4
Peter A-C Erzeugung rekombinanter Antikörper gegen das Zentromerprotein CENH3 Alpha. (Bachelor Thesis) Emden, Hochschule Emden/Leer, Fachbereich Technik (2014) 42 pp.
Phan H T, Hause B, Hause G, Arcalis E, Stoger E, Maresch D, Altmann F, Joensuu J, Conrad U Influence of elastin-like polypeptide and hydrophobin on recombinant hemagglutinin accumulations in transgenic tobacco plants. PLoS One 9 (2014) e99347. dx.doi.org/10.1371/journal.pone.0099347
Saalbach I, Mora-Ramírez I, Weichert N, Andersch F, Guild G, Wieser H, Koehler P, Stangoulis J, Kumlehn J, Weschke W, Weber H Increased grain yield and micronutrient concentration in transgenic winter wheat by ectopic expression of a barley sucrose transporter. J. Cereal Sci. 60 (2014) 75-81. dx.doi.org/10.1016/j.jcs.2014.01.017
Weichert N, Hauptmann V, Conrad U Transglutamination enables production and characterization of native-sized spider silk-ELP fusion proteins from genetically engineered plants. ISB News Report (2014) 5-8.
Weichert N, Hauptmann V, Menzel M, Schallau K, Gunkel P, Hertel T C, Pietzsch M, Spohn U, Conrad U Transglutamination allows production and characterization of native-sized ELPylated spider silk proteins from transgenic plants. Plant Biotechnol. J. 12 (2014) 265-275. dx.doi.org/10.1111/pbi.12135
Zábrady M, Hrdinová V, Müller B, Conrad U, Hejátko J, Janda L Targeted in vivo inhibition of specific protein-protein interactions using recombinant antibodies. PLoS One 9 (2014) e109875. dx.doi.org/10.1371/journal.pone.0109875
2013
Floss D M, Conrad U, Rose-John S, Scheller J ELP fusion technology for biopharmaceuticals. In: Schmidt S R (Ed.): Fusion protein technologies for biopharmaceuticals: applications and challenges. Hoboken, NJ: Wiley (2013) 211-226. dx.doi.org/10.1002/9781118354599.ch14 ISBN 978-0-470-64627-4
Hauptmann V, Weichert N, Menzel M, Knoch D, Paege N, Scheller J, Spohn U, Conrad U, Gils M Native-sized spider silk proteins synthesized in planta via intein-based multimerization. Transgenic Res. 22 (2013) 369-377. dx.doi.org/10.1007/s11248-012-9655-6
Hauptmann V, Weichert N, Rakhimova M, Conrad U Spider silks from plants - a challenge to create native-sized spidroins. Biotechnol. J. 8 (2013) 1183-1192. dx.doi.org/10.1002/biot.201300204
Heinze M, Herre M, Massalski C, Hermann I, Conrad U, Roos W Signal transfer in the plant plasma membrane: phospholipase A2 is regulated via an inhibitory Gα protein and a cyclophilin. Biochem. J. 450 (2013) 497-509. dx.doi.org/10.1042/Bj20120793
Phan H T, Floss D M, Conrad U Veterinary vaccines from transgenic plants: highlights of two decades of research and a promising example. Curr. Pharm. Des. 19 (2013) 5601-5611. dx.doi.org/10.2174/1381612811319310014
Phan H T, Pohl J, Floss D M, Rabenstein F, Veits J, Le B T, Chu H H, Hause G, Mettenleiter T, Conrad U ELPylated haemagglutinins produced in tobacco plants induce potentially neutralizing antibodies against H5N1 viruses in mice. Plant Biotechnol. J. 11 (2013) 582-593. dx.doi.org/10.1111/pbi.12049
Sorge E Herstellung und Charakterisierung einer Komponente des Proteinabbauweges in Pflanzen. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2013) 46 pp.
2012
Floss D M, Conrad U Plant molecular pharming – veterinary applications. In: Meyers R A (Ed.): Encyclopedia of sustainability science and technology, Vol. 11. (Series: Springer reference) New York [u.a.]: Springer (2012) 8073-8080. dx.doi.org/10.1007/978-1-4419-0851-3_270 ISBN 978-1-441-90852-0
Hauptmann V, Gils M, Conrad U, Phan H T Methods of producing and purifying polymeric proteins in transgenic plants (Patent). EP 2518081, Granted on 31.10.2012, IPK-Nr. 2011/07 (2012).
Hoang P T ELPylated avian flu vaccines from plants: Improvement of expression and development of a new purification strategy. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2012) 128 pp.
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi T M, Renou J P, Balzergue S, Viehover P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana. Plant J. 71 (2012) 427-442. dx.doi.org/10.1111/j.1365-313X.2012.04999.x
Knopf J Produktion, Reinigung und immunologische Charakterisierung von Vogelgrippe-Neuraminidaseantigenen aus transgenem Tabak. (Master Thesis) Köthen, Hochschule Anhalt (FH) (2012) 86 pp.
Mönke G, Seifert M, Keilwagen J, Mohr M, Grosse I, Hähnel U, Junker A, Weisshaar B, Conrad U, Bäumlein H, Altschmied L Toward the identification and regulation of the Arabidopsis thaliana ABI3 regulon. Nucleic Acids Res. 40 (2012) 8240-8254. dx.doi.org/10.1093/nar/gks594
Njo D Expression and characterization of the TB antigen (Ag85B) hydrophobin fusion protein in tobacco (Nicotiana benthamiana) leaves. (Master Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Masterkurs für Pharmazeutische Biotechnologie (2012) 76 pp.
Paege N Produktion und Charakterisierung von ELP-Multimeren in Pflanzen. (Diploma Thesis) Berlin, Technische Universität (2012) 109 pp.
Staroske N ABA-Immunmodulierung sich entwickelnder Gerstenkaryopsen. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2012) 147 pp.
2011
Banik A Funktionelle Charakterisierung von rekombinanten Antikörpern gegen Abszisinsäurerezeptoren. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2011) 55 pp.
Conrad U, Plagmann I, Malchow S, Sack M, Floss D M, Kruglov A A, Nedospasov S A, Rose-John S, Scheller J ELPylated anti-human TNF therapeutic single-domain antibodies for prevention of lethal septic shock. Plant Biotechnol. J. 9 (2011) 22–31. dx.doi.org/10.1111/j.1467-7652.2010.00523.x
Knoch D Expression, Reinigung und Charakterisierung von Spinnenseidenproteinmultimeren aus Tabak. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2011) 65 pp.
Mielke K, Forner S, Kramell R, Conrad U, Hause B Cell-specific visualization of jasmonates in wounded tomato and Arabidopsis leaves using jasmonate-specific antibodies. New Phytol. 190 (2011) 1069-1080. dx.doi.org/10.1111/j.1469-8137.2010.03638.x
Phan H T, Conrad U Membrane-based inverse transition cycling: an improved means for purifying plant-derived recombinant protein-elastin-like polypeptide fusions. Int. J. Mol. Sci. 12 (2011) 2808-2821. dx.doi.org/10.3390/ijms12052808
Pohl J Produktion von oligomeren Vogelgrippeantigenen als ELP-Fusionsproteine in Pflanzen. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2011) 67 pp.
Rother E Herstellung und Charakterisierung von scFv´s gegen den Phytohormonrezeptor RCAR1. (Bachelor Thesis) Mittweida, Hochschule Mittweida (FH) (2011) 69 pp.
Saumonneau A, Rottier K, Conrad U, Popineau Y, Francin-Allami M, Gueguen J Expression of a new chimeric protein with a highly repeated sequence in tobacco cells. Plant Cell Rep. 30 (2011) 1289-1302. dx.doi.org/10.1007/s00299-011-1040-z
Ziermann H Untersuchungen zur Sequenzspezifität der Bindung von Transkriptionsfaktoren an DNA. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2011) 48 pp.
2010
Conrad U, Floss D M Expression of antibody fragments in transgenic plants. In: Kontermann R, Dübel S (Eds.): Antibody engineering, Vol. 2 (2nd edition). Heidelberg [u.a.]: Springer (2010) 377-386. dx.doi.org/10.1007/978-3-642-01147-4_29 ISBN 978-3-642-01146-7
Floss D M, Conrad U Expression of complete antibodies in transgenic plants. In: Kontermann R, Dübel S (Eds.): Antibody engineering, Vol. 1 (2nd edition). Berlin [u.a.]: Springer (2010) 489-502. dx.doi.org/10.1007/978-3-642-01144-3_31 ISBN 978-3-642-01143-6
Floss D M, Mockey M, Zanello G, Brosson D, Diogon M, Frutos R, Bruel T, Rodrigues V, Garzon E, Chevaleyre C, Berri M, Salmon H, Conrad U, Dedieu L Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in transgenic plants by Elastin-like peptide fusion strategy. J. Biomed. Biotechnol. 2010 (2010) 274346. dx.doi.org/10.1155/2010/274346
Floss D M, Schallau K, Rose-John S, Conrad U, Scheller J Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application. Trends Biotechnol. 28 (2010) 37-45. dx.doi.org/10.1016/j.tibtech.2009.10.004
Gastaldello J Funktionelle Charakterisierung neuer anti hTNFα VHH cameloid nanobodies. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2010) 84 pp.
Giersberg M, Floss D M, Kipriyanov S, Conrad U, Scheller J Covalent dimerization of camelidae anti-human TNF-alpha single domain antibodies by the constant kappa light chain domain improves neutralizing activity. Biotechnol. Bioeng. 106 (2010) 161-166. dx.doi.org/10.1002/bit.22653
Kunze M Charakteriserung des Abscisinsäure-Rezeptor bindenden Antikörpers anti-RCAR1. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Institut für Biochemie und Biotechnologie (2010) 50 pp.
Radchuk R, Conrad U, Saalbach I, Giersberg M, Emery R J, Küster H, Nunes-Nesi A, Fernie A R, Weschke W, Weber H Abscisic acid deficiency of developing pea embryos achieved by immunomodulation attenuates developmental phase transition and storage metabolism. Plant J. 64 (2010) 715-730. dx.doi.org/10.1111/j.1365-313X.2010.04376.x
Vogel M, Lawson M, Sippl W, Conrad U, Roos W Structure and mechanism of sanguinarine reductase, an enzyme of alkaloid detoxification. J. Biol. Chem. 285 (2010) 18397-18406. dx.doi.org/10.1074/jbc.M109.088989
2009
Floss D M, Kumlehn J, Conrad U, Saalbach I Haploid technology allows for the efficient and rapid generation of homozygous antibody-accumulating transgenic tobacco plants. Plant Biotechnol. J. 7 (2009) 593-601. dx.doi.org/10.1111/j.1467-7652.2009.00426.x
Floss D M, Sack M, Arcalis E, Stadlmann J, Quendler H, Rademacher T, Stoger E, Scheller J, Fischer R, Conrad U Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody. Plant Biotechnol. J. 7 (2009) 899-913. dx.doi.org/10.1111/j.1467-7652.2009.00452.x
Gahrtz M, Conrad U Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies. In: Faye L, Gomord V (Eds.): Recombinant proteins from plants: methods and protocols. (Series: Methods in molecular biology, Vol. 483) Totowa, NJ: Humana Press (2009) 289-312. dx.doi.org/10.1007/978-1-59745-407-0_17 ISBN 978-1-588-29978-9
Hallmann S Funktionelle und physische Charakterisierung von nanobodies (cameloid VHH). (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2009) 67 pp.
Jahn D, Matros A, Bakulina A Y, Tiedemann J, Schubert U, Giersberg M, Haehnel S, Zoufal K, Mock H-P, Kipriyanov S M Model structure of the immunodominant surface antigen of Eimeria tenella identified as a target for sporozoite-neutralizing monoclonal antibody. Parasitol. Res. 105 (2009) 655-668. dx.doi.org/10.1007/s00436-009-1437-6
Kretzschmar A Einfluss der bZIP-Transkriptionsfaktoren ABI5, bZIP67 und EEL auf die ABI3-regulierte Genexpression. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2009) 70 pp.
Zimmermann J, Saalbach I, Jahn D, Giersberg M, Haehnel S, Wedel J, Macek J, Zoufal K, Glünder G, Falkenburg D, Kiprijanov S M Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens. BMC Biotechnol. 9 (2009) 79. dx.doi.org/10.1186/1472-6750-9-79
2008
Floss D M Expression von anti-HIV-1 Antikörper-ELP-Fusionen in Nicotiana tabacum - Biochemische und funktionelle Charakterisierung. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Biowissenschaften (2008) 150 pp.
Floss D M, Sack M, Stadlmann J, Rademacher T, Scheller J, Stöger E, Fischer R, Conrad U Biochemical and functional characterization of anti-HIV antibody-ELP fusion proteins from transgenic plants. Plant Biotechnol. J. 6 (2008) 379-391. dx.doi.org/10.1111/j.1467-7652.2008.00326.x
Frutos R, Denise H, Vivares C, Neuhaus J M, Vitale S, Pedrazzini E, Ma J, Dix P, Gray J, Pezzotti M, Conrad U, Robinson D Pharmaceutical proteins in plants: a strategic genetic engineering approach for the production of tuberculosis antigens. Ann. N. Y. Acad. Sci. 1149 (2008) 275-280. dx.doi.org/10.1196/annals.1428.089
Irons S L, Nuttall J, Floß D M, Frigerio L, Kotzer A M, Hawes C Fluorescent protein fusions to a human immunodeficiency virus monoclonal antibody reveal its intracellular transport through the plant endomembrane system. Plant Biotechnol. J. 6 (2008) 649-662. dx.doi.org/10.1111/j.1467-7652.2008.00348.x
Junghans F, Conrad U, Heilmann A, Spohn U Micromechanical investigations on films made of recombinant spider silk proteins and silk fibroin mater. In: Protein and peptide engineering for therapeutic and functional materials: November 26 - 30, 2007, Boston, USA, symposium proceedings. (Series: Materials Research Society symposium proceedings, Vol. 1062) Red Hook, NY, USA: Curran (2008) 1006-1008. ISBN 978-1-60560-836-5
Nguyen L T Identification of stress-responsive ABA-dependent and ABA-independent transcription factors in Arabidopsis thaliana plants with immunomodulated ABA function. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2008) 128 pp.
Schallau K Herstellung von Spinnenseidenproteinen in Tabaksamen. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Biowissenschaften (2008) 100 pp.
Tiedemann J, Rutten T, Mönke G, Vorwieger A, Rolletschek H, Meissner D, Milkowski C, Petereck S, Mock H-P, Zank T, Bäumlein H Dissection of a complex seed phenotype: novel insights of FUSCA3 regulated developmental processes. Dev. Biol. 317 (2008) 1-12. dx.doi.org/10.1016/j.ydbio.2008.01.034
Tran M L Functional characterisation of the transcription factors AtMYB44 and AtMYB77 and identification of putative target gene promoters in Arabidopsis thaliana. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität, Mathematisch-Naturwissenschaftliche Fakultät (2008) 162 pp.
Urakami E, Yamaguchi I, Asami T, Conrad U, Suzuki Y Immunomodulation of gibberellin biosynthesis using an anti-precursor gibberellin antibody confers gibberellin-deficient phenotypes. Planta 228 (2008) 863-873. dx.doi.org/10.1007/s00425-008-0788-z
2007
Floss D M, Falkenburg D, Conrad U Production of vaccines and therapeutic antibodies for veterinary applications in transgenic plants: an overview. Transgenic Res. 16 (2007) 315-332. dx.doi.org/10.1007/s11248-007-9095-x
Mönke G, Linh T M, Conrad U, Hähnel U, Altschmied L, Mohr M, Grosse I, Vorwieger A, Bäumlein H, Weisshaar B, Viehöver P GABI-ARABIDO-SEED: Wie steuern Transkriptionsfaktoren die Samenentwicklung bei Pflanzen? GenomXPress Sonderausgabe März (2007) 16.
Rakhimova M Expression of spider silk and spider silk-like proteins in potato and tobacco. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2007) 93 pp.
Ten Hoopen P, Hunger A, Muller A, Hause B, Kramell R, Wasternack C, Rosahl S, Conrad U Immunomodulation of jasmonate to manipulate the wound response. J. Exp. Bot. 58 (2007) 2525–2535. dx.doi.org/10.1093/Jxb/Erm122
2006
Junghans F, Morawietz M, Conrad U, Scheibel T, Heilmann A, Spohn U Preparation and mechanical properties of layers made of recombinant spider silk proteins. Appl. Physics A 82 (2006) 253-260. dx.doi.org/10.1007/s00339-005-3432-9
Kovaleva M, Bussmeyer I, Rabe B, Grötzinger J, Sudarman E, Eichler J, Conrad U, Rose-John S, Scheller J Abrogation of viral interleukin-6 (vIL-6)-induced signaling by intracellular retention and neutralization of vIL-6 with an anti-vIL-6 single-chain antibody selected by phage display. J. Virol. 80 (2006) 8510-8520. dx.doi.org/10.1128/Jvi.00420-06
Lin M, Rose-John S, Grötzinger J, Conrad U, Scheller J Functional expression of a biologically active fragment of soluble gp130 as an ELP-fusion protein in transgenic plants: purification via inverse transition cycling. Biochem. J. 398 (2006) 577-583. dx.doi.org/10.1042/Bj20060544
Scheller J, Leps M, Conrad U Forcing single-chain variable fragment production in tobacco seeds by fusion to elastin-like polypeptides. Plant Biotechnol. J. 4 (2006) 243-249. dx.doi.org/10.1111/j.1467-7652.2005.00176.x