Publikationen

Autoren Titel
2019
Beier S, Ulpinnis C, Schwalbe M, Münch T, Hoffie R, Koeppel I, Hertig C, Budhagatapalli N, Hiekel S, Pathi K M, Hensel G, Grosse M, Chamas S, Gerasimova S, Kumlehn J, Scholz U, Schmutzer T Kmasker plants - a tool for assessing complex sequence space in plant species. Plant J. (2019) accepted. dx.doi.org/10.1111/tpj.14645
Frank S, Hollmann J, Mulisch M, Matros A, Carrión C C, Mock H P, Hensel G, Krupinska K Barley cysteine protease PAP14 plays a role in degradation of chloroplast proteins. J. Exp. Bot. 70 (2019) 6057-6069. dx.doi.org/10.1093/jxb/erz356
Gomez-Sanchez A, Gonzalez-Melendi P, Santamaria M E, Arbona V, Lopez-Gonzalvez A, Garcia A, Hensel G, Kumlehn J, Martinez M, Diaz I Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses. J. Exp. Bot. 70 (2019) 2143-2155. dx.doi.org/10.1093/jxb/ery410
Hensel G Genetic transformation of Triticeae cereals – Summary of almost three-decade's development. Biotechnol. Adv. (2019) in press 107484. doi.org/10.1016/j.biotechadv.2019.107484
Hensel G, Kumlehn J Genome engineering using TALENs. In: Harwood W A (Ed.): Barley: methods and protocols. (Series: Methods in molecular biology, Vol. 1900) New York, NY: Humana Press (2019) 195-215. dx.doi.org/10.1007/978-1-4939-8944-7_13 978-1-4939-8944-7
Kalinowska K, Chamas S, Unkel K, Demidov D, Lermontova I, Dresselhaus T, Kumlehn J, Dunemann F, Houben A State-of-the-art and novel developments of in vivo haploid technologies. Theor. Appl. Genet. 132 (2019) 593–605. dx.doi.org/10.1007/s00122-018-3261-9
Koeppel I, Hertig C, Hoffie R, Kumlehn J Cas endonuclease technology - a quantum leap in the advancement of barley and wheat genetic engineering. Int. J. Mol. Sci. 20 (2019) 2647. dx.doi.org/10.3390/ijms20112647
Koeppel I, Hertig C, Hoffie R, Kumlehn J Cas endonuclease technology for plant research and breeding. Encyclopedia (2019) v2. encyclopedia.pub/196
Krattinger S G, Kang J, Bräunlich S, Boni R, Chauhan H, Selter L L, Robinson M D, Schmid M W, Wiederhold E, Hensel G, Kumlehn J, Sucher J, Martinoia E, Keller B Abscisic acid is a substrate of the ABC transporter encoded by the durable wheat disease resistance gene Lr34. New Phytol. 223 (2019) 853-866. dx.doi.org/10.1111/nph.15815
Krupinska K, Braun S, Nia M S, Schäfer A, Hensel G, Bilger W The nucleoid-associated protein WHIRLY1 is required for the coordinate assembly of plastid and nucleus-encoded proteins during chloroplast development. Planta 249 (2019) 1337-1347. dx.doi.org/10.1007/s00425-018-03085-z
Kumlehn J Site-directed genome modification in Triticeae cereals mediated by Cas endonucleases. In: Miedaner T, Korzun V (Eds.): Applications of genetic and genomic research in cereals. (Woodhead Publishing Series in Food Science, Technology and Nutrition) Duxford, UK u.a.: Elsevier Ltd. (2019) 121-136. ISBN 978-0-08-102163-7
Kumlehn J, Hiekel S, Budhagatapalli N Site-directed genome modification in barley and wheat. In: Ordon F, Friedt W (Eds.): Advances in breeding techniques for cereal crops. (Series: Burleigh dodds series in agricultural science, Vol. 60) Cambridge, UK: Burleigh Dodds Science Pub LTD (2019) ISBN 978-1-78676-244-3
Li M, Hensel G, Mascher M, Melzer M, Budhagatapalli N, Rutten T, Himmelbach A, Beier S, Korzun V, Kumlehn J, Boerner T, Stein N Leaf variegation and impaired chloroplast development caused by a truncated CCT domain gene in albostrians barley. Plant Cell 31 (2019) 1430-1445. dx.doi.org/10.1105/tpc.19.00132
Marzec M, Hensel G More precise, more universal and more specific – the next generation of RNA-guided endonucleases for genome editing. FEBS J. 286 (2019) 4657–4660. dx.doi.org/10.1111/febs.15079
Pouramini P, Fotokian M H, Dehghan H, Hensel G Effect of Thiobacillus and superabsorbent on essential oil components in Thyme species. J Essent. Oil Bearing Plants 22 (2019) 799-810. dx.doi.org/10.1080/0972060X.2019.1623086
Sakuma S, Golan G, Guo Z, Ogawa T, Tagiri A, Sugimoto K, Bernhardt N, Brassac J, Mascher M, Hensel G, Ohnishi S, Jinno H, Yamashita Y, Ayalon I, Peleg Z, Schnurbusch T, Komatsuda T Unleashing floret fertility in wheat through the mutation of a homeobox gene. Proc. Natl. Acad. Sci. U.S.A. 116 (2019) 5182-5187. dx.doi.org/10.1073/pnas.1815465116
Wang Y, Subedi S, de Vries H, Doornenbal P, Vels A, Hensel G, Kumlehn J, Johnston P A, Qi X, Blilou I, Niks R E, Krattinger S G Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi. Nat. Plants 5 (2019) 1129-1135. dx.doi.org/10.1038/s41477-019-0545-2
2018
Boni R, Chauhan H, Hensel G, Roulin A, Sucher J, Kumlehn J, Brunner S, Krattinger S G, Keller B Pathogen-inducible Ta-Lr34res expression in heterologous barley confers disease resistance without negative pleiotropic effects. Plant Biotechnol. J. 16 (2018) 245-253. dx.doi.org/10.1111/pbi.12765
Gerasimova S V, Korotkova A M, Hertig C, Hiekel S, Hoffie R, Budhagatapalli N, Otto I, Hensel G, Shumny V K, Kochetov A V, Kumlehn J, Khlestkina E K Targeted genome modification in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease. Vavilov J. Genet. Breed. 22 (2018) 1033-1039. dx.doi.org/10.18699/VJ18.447
Holubová K, Hensel G, Vojta P, Tarkowski P, Bergougnoux V, Galuszka P Modification of barley plant productivity through regulation of cytokinin content by reverse-genetics approaches. Front. Plant Sci. 9 (2018) 1676. dx.doi.org/10.3389/fpls.2018.01676
Käsbauer C L, Pathuri I P, Hensel G, Kumlehn J, Hückelhoven R, Proels R K Barley ADH-1 modulates susceptibility to Bgh and is involved in chitin-induced systemic resistance. Plant Physiol. Biochem. 123 (2018) 281-287. doi.org/10.1016/j.plaphy.2017.12.029
Kumlehn J, Pietralla J, Hensel G, Pacher M, Puchta H The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology. J. Integr. Plant Biol. 60 (2018) 1127-1153. dx.doi.org/10.1111/jipb.12734
Li B, Förster C, Robert C A M, Zust T, Hu L, Machado R A R, Berset J D, Handrick V, Knauer T, Hensel G, Chen W, Kumlehn J, Yang P, Keller B, Gershenzon J, Jander G, Köllner T G, Erb M Convergent evolution of a metabolic switch between aphid and caterpillar resistance in cereals. Sci. Adv. 4 (2018) eaat6797. dx.doi.org/10.1126/sciadv.aat6797
Marzec M, Hensel G Targeted base editing systems are available for plants. Trends Plant Sci. 23 (2018) 955-957. dx.doi.org/10.1016/j.tplants.2018.08.011
Pfrieme A-K Untersuchungen der Effizienz RNA-vermittelter Cas-Endonukleasen-induzierter Mutagenese in Abhängigkeit von Cas9 Codon-Optimierung, verwendetem Promotor und single guide RNA-Startmolekül. (Master Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Institut für Agrar- und Ernährungswissenschaften der Naturwissenschaftlichen Fakultät III (2018) 135 + XXV pp.
Radchuk V, Tran V, Radchuk R, Diaz-Mendoza M, Weier D, Fuchs J, Riewe D, Hensel G, Kumlehn J, Munz E, Heinzel N, Rolletschek H, Martinez M, Borisjuk L Vacuolar processing enzyme 4 contributes to maternal control of grain size in barley by executing programmed cell death in the pericarp. New Phytol. 218 (2018) 1127-1142. dx.doi.org/10.1111/nph.14729
Rajaraman J, Douchkov D, Lück S, Hensel G, Nowara D, Pogoda M, Rutten T, Meitzel T, Brassac J, Höfle C, Hückelhoven R, Klinkenberg J, Trujillo M, Bauer E, Schmutzer T, Himmelbach A, Mascher M, Lazzari B, Stein N, Kumlehn J, Schweizer P Evolutionarily conserved partial gene duplication in the Triticeae tribe of grasses confers pathogen resistance. Genome Biol. 19 (2018) 116. dx.doi.org/10.1186/s13059-018-1472-7
Santamaria M E, Diaz-Mendoza M, Perez-Herguedas D, Hensel G, Kumlehn J, Diaz I, Martinez M Overexpression of HvIcy6 in barley enhances resistance against Tetranychus urticae and entails partial transcriptomic reprogramming. Int. J. Mol. Sci. 19 (2018) 697. dx.doi.org/10.3390/ijms19030697
Świda-Barteczka A, Krieger-Liszkay A, Bilger W, Voigt U, Hensel G, Szweykowska-Kulinska Z, Krupinska K The plastid-nucleus located DNA/RNA binding protein WHIRLY1 regulates microRNA-levels during stress in barley (Hordeum vulgare L.). RNA Biol. 15 (2018) 886-891. dx.doi.org/10.1080/15476286.2018.1481695
Velasco-Arroyo B, Diaz-Mendoza M, Gomez-Sanchez A, Moreno-Garcia B, Santamaria M E, Torija-Bonilla M, Hensel G, Kumlehn J, Martinez M, Diaz I Silencing barley cystatins HvCPI-2 and HvCPI-4 specifically modifies leaf responses to drought stress. Plant Cell Environ. 41 (2018) 1776-1790. dx.doi.org/10.1111/pce.13178
2017
Audije de la Fuente S Production of barley (Hordeum vulgare L.) doubled haploids via interspecific crosses. (Diploma Thesis) Madrid, Universidad Politécnica de Madrid (2017) 133 pp.
Dirks J Untersuchung des Einflusses der ATG-Kontext-Sequenz auf die Translationseffizienz. (Master Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät III, Institut für Agrar- und Ernährungswissenschaften (2017) 47 pp.
Grosse M Gerichtete Mutagenese Methionin-Aminopeptidase-codierender Gene durch gRNA-vermittelte Cas9-Endonuklease. (Master Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2017) 123 pp.
Gurushidze M, Hiekel S, Otto I, Hensel G, Kumlehn J Site-directed mutagenesis in barley by expression of TALE nuclease in embryogenic pollen. In: Jankowicz-Cieslak J, Tai T, Kumlehn J, Till B J (Eds.): Biotechnologies for plant mutation breeding: protocols. Cham: Springer (2017) 113-128. dx.doi.org/10.1007/978-3-319-45021-6_7 ISBN 978-3-319-45019-3
Gurushidze M, Trautwein H, Hoffmeister P, Otto I, Müller A, Kumlehn J Doubled haploidy as a tool for chimera dissolution of TALEN induced mutations in barley. In: Jankowicz-Cieslak J, Tai T, Kumlehn J, Till B J (Eds.): Biotechnologies for plant mutation breeding: protocols. Cham: Springer (2017) 129-141. dx.doi.org/10.1007/978-3-319-45021-6_8 ISBN 978-3-319-45019-3
Hensel G, Marthe C, Kumlehn J Agrobacterium-mediated transformation of wheat using immature embryos. In: Bhalla P L, Singh M B (Eds.): Wheat biotechnology: methods and protocols. (Series: Methods in molecular biology, Vol. 1679) New York [u.a.]: Humana Press (2017) 129-139. dx.doi.org/10.1007/978-1-4939-7337-8_8 ISBN 978-1-4939-7335-4
Hohmann J Die Anwendung der gRNA/Cas9-induzierten Mutagenese in nahrungsrelevanten Nutzpflanzenarten. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät III, Institut für Agrar- und Ernährungswissenschaften (2017) 53 pp.
Jankowicz-Cieslak J, Tai T, Kumlehn J, Till B J (Eds.) Biotechnologies for plant mutation breeding: protocols. Cham: Springer International Publishing (2017) 339 pp. ISBN 978-3-319-45019-3
Kucharewicz W, Distelfeld A, Bilger W, Müller M, Munné-Bosch S, Hensel G, Krupinska K Acceleration of leaf senescence is slowed down in transgenic barley plants deficient in the DNA/RNA-binding protein WHIRLY1. J. Exp. Bot. 68 (2017) 983-996. dx.doi.org/10.1093/jxb/erw501
Lo Presti L, Zechmann B, Kumlehn J, Liang L, Lanver D, Tanaka S, Bock R, Kahmann R An assay for entry of secreted fungal effectors into plant cells. New Phytol. 213 (2017) 956-964. dx.doi.org/10.1111/nph.14188
Pandey P, Daghma D S, Houben A, Kumlehn J, Melzer M, Rutten T Dynamics of post-translationally modified histones during barley pollen embryogenesis in the presence or absence of the epi-drug trichostatin A. Plant Reprod. 30 (2017) 95-105. dx.doi.org/10.1007/s00497-017-0302-5
Schedel S Genetische Veränderung von Tabak mittels Zielsequenz-spezifizierter Endonukleasen. (PhD Thesis) Hannover, Leibniz Universität Hannover, Naturwissenschaftliche Fakultät (2017) 133 pp.
Schedel S, Pencs S, Hensel G, Müller A, Rutten T, Kumlehn J RNA-guided Cas9-induced mutagenesis in tobacco followed by efficient genetic fixation in doubled haploid plants. Front. Plant Sci. 7 (2017) 1995. dx.doi.org/10.3389/fpls.2016.01995
Sucher J, Boni R, Yang P, Rogowsky P, Büchner H, Kastner C, Kumlehn J, Krattinger S G, Keller B The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize. Plant Biotechnol. J. 15 (2017) 489–496. dx.doi.org/10.1111/pbi.12647
Thirulogachandar V, Alqudah A M, Koppolu R, Rutten T, Graner A, Hensel G, Kumlehn J, Bräutigam A, Sreenivasulu N, Schnurbusch T, Kuhlmann M Leaf primordium size specifies leaf width and vein number among row-type classes in barley. Plant J. 91 (2017) 601-612. dx.doi.org/10.1111/tpj.13590
Till B J, Hofinger B J, Sen A, Huynh O A, Jankowicz-Cieslak J, Gugsa L, Kumlehn J A protocol for validation of doubled haploid plants by enzymatic mismatch cleavage. In: Jankowicz-Cieslak J, Tai T, Kumlehn J, Till B J (Eds.): Biotechnologies for plant mutation breeding: protocols. Cham: Springer (2017) 253-262. dx.doi.org/10.1007/978-3-319-45021-6_16 ISBN 978-3-319-45019-3
Wendler N, Mascher M, Himmelbach A, Bini F, Kumlehn J, Stein N A high-density, sequence-enriched genetic map of Hordeum bulbosum and its collinearity to H. vulgare. Plant Genome 10 (2017) dx.doi.org/10.3835/plantgenome2017.06.0049
2016
Budhagatapalli N, Schedel S, Gurushidze M, Pencs S, Hiekel S, Rutten T, Kusch S, Morbitzer R, Lahaye T, Panstruga R, Kumlehn J, Hensel G A simple test for the cleavage activity of customized endonucleases in plants. Plant Methods 12 (2016) 18. dx.doi.org/10.1186/s13007-016-0118-6
Chen W, Kastner C, Nowara D, Oliveira-Garcia E, Rutten T, Zhao Y, Deising H B, Kumlehn J, Schweizer P Host-induced silencing of Fusarium culmorum genes protects wheat from infection. J. Exp. Bot. 67 (2016) 4979-4991. dx.doi.org/10.1093/jxb/erw263
Diaz-Mendoza M, Dominguez-Figueroa J D, Velasco-Arroyo B, Cambra I, Gonzalez-Melendi P, Lopez-Gonzalvez A, Garcia A, Hensel G, Kumlehn J, Diaz I, Martinez M HvPap-1 C1A protease and HvCPI-2 cystatin contribute to barley grain filling and germination. Plant Physiol. 170 (2016) 2511-2524. dx.doi.org/10.1104/pp.15.01944
Douchkov D, Lueck S, Hensel G, Kumlehn J, Rajaraman J, Johrde A, Doblin M S, Beahan C T, Kopischke M, Fuchs R, Lipka V, Niks R E, Bulone V, Chowdhury J, Little A, Burton R A, Bacic A, Fincher G B, Schweizer P The barley (Hordeum vulgare) cellulose synthase-like D2 gene (HvCslD2) mediates penetration resistance to host-adapted and nonhost isolates of the powdery mildew fungus. New Phytol. 212 (2016) 421-433. dx.doi.org/10.1111/nph.14065
Ezura H, Ariizumi T, Garcia-Mas J, Rose J (Eds.) Functional Genomics and Biotechnology in Solanaceae and Cucurbitaceae Crops. (Series: Widholm J., J. Kumlehn & T. Nagata (Eds.): Biotechnology in agriculture and forestry, Vol. 70) Berlin Heidelberg: Springer (2016) 265 pp. ISBN 978-3-662-48533-0
Houben A, Karimi Ashtiyani R, Ishii T, Stein N, Kumlehn J Generation of haploid plants. (Industrieanmeldung), Veröffentlichung: 03.03.2016, IPK-Nr. 2014/01. EP 14182719.6 (2016).
Jöst M, Hensel G, Kappel C, Druka A, Sicard A, Hohmann U, Beier S, Himmelbach A, Waugh R, Kumlehn J, Stein N, Lenhard M The INDETERMINATE DOMAIN protein BROAD LEAF1 limits barley leaf width by restricting lateral proliferation. Curr. Biol. 26 (2016) 903-909. dx.doi.org/10.1016/j.cub.2016.01.047
Kumlehn J Embryogenesis and plant regeneration from isolated wheat zygotes. In: Germanà M A, Lambardi M (Eds.): In vitro embryogenesis in higher plants. (Series: Methods in molecular biology, Vol. 1359) New York, NY [u.a.]: Humana Press (2016) 503-514. dx.doi.org/10.1007/978-1-4939-3061-6_29 ISBN 978-1-4939-3060-9
Marzin S, Hanemann A, Sharma S, Hensel G, Kumlehn J, Schweizer G, Röder M S Are PECTIN ESTERASE INHIBITOR genes involved in mediating resistance to Rhynchosporium commune in barley? PLoS One 11 (2016) e0150485. dx.doi.org/10.1371/journal.pone.0150485
Nakamura S, Pourkheirandish M, Morishige H, Kubo Y, Nakamura M, Ichimura K, Seo S, Kanamori H, Wu J, Ando T, Hensel G, Sameri M, Stein N, Sato K, Matsumoto T, Yano M, Komatsuda T Mitogen-Activated Protein Kinase 3 regulates seed dormancy in barley. Curr. Biol. 26 (2016) 775-781. dx.doi.org/10.1016/j.cub.2016.01.024
Rabe F, Bosch J, Stirnberg A, Guse T, Bauer L, Seitner D, Rabanal F A, Czedik-Eysenberg A, Uhse S, Bindics J, Genenncher B, Navarrete F, Kellner R, Ekker H, Kumlehn J, Vogel J P, Gordon S P, Marcel T C, Münsterkötter M, Walter M C, Sieber C M K, Mannhaupt G, Güldener U, Kahmann R, Djamei A A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem. eLife 5 (2016) e20522. dx.doi.org/10.7554/eLife.20522
Rajaraman J, Douchkov D, Hensel G, Stefanato F, Gordon A, Ereful N, Caldararu O, Petrescu A-J, Kumlehn J, Boyd L, Schweizer P An LRR/malectin receptor-like kinase mediates resistance to non-adapted and adapted powdery mildew fungi in barley and wheat. Front. Plant Sci. 7 (2016) 1836. dx.doi.org/10.3389/fpls.2016.01836
Seifert F, Bössow S, Kumlehn J, Gnad H, Scholten S Analysis of wheat microspore embryogenesis induction by transcriptome and small RNA sequencing using the highly responsive cultivar Svilena"." BMC Plant Biol. 16 (2016) 97. dx.doi.org/10.1186/s12870-016-0782-8
Staroske N, Conrad U, Kumlehn J, Hensel G, Radchuk R, Erban A, Kopka J, Weschke W, Weber H Increasing abscisic acid levels by immunomodulation in barley grains induces precocious maturation without changing grain composition. J. Exp. Bot. 67 (2016) 2675-2687. dx.doi.org/10.1093/jxb/erw102
Trautwein H Etablierung eines in planta Funktionalitätstests für Zielsequenz-spezifisch programmierbare Endonukleasen. (Bachelor Thesis) Hamburg, Hochschule für Angewandte Wissenschaften (2016) 94 pp.
Velasco-Arroyo B, Diaz-Mendoza M, Gandullo J, Gonzalez-Melendi P, Santamaria M E, Dominguez-Figueroa J D, Hensel G, Martinez M, Kumlehn J, Diaz I HvPap-1 C1A protease actively participates in barley proteolysis mediated by abiotic stresses. J. Exp. Bot. 67 (2016) 4267-4310. dx.doi.org/10.1093/jxb/erw212
Watanabe K, Breier U, Hensel G, Kumlehn J, Schubert I, Reiss B Stable gene replacement in barley by targeted double-strand break induction. J. Exp. Bot. 67 (2016) 1433-1445. dx.doi.org/10.1093/jxb/erv537
Weidenbach D, Esch L, Möller C, Hensel G, Kumlehn J, Höfle C, Hückelhoven R, Schaffrath U Polarized defence against fungal pathogens is mediated by the Jacalin-Lectin domain of modular Poaceae-specific proteins. Mol. Plant 9 (2016) 514-527. dx.doi.org/10.1016/j.molp.2015.12.009