Development of CRISPR-based methods suitable for life imaging of chromosomes
Elucidating how the genome is spatio-temporally organised inside the nucleus is imperative to understand the regulation of genes and non-coding sequences during development. Emerging techniques of chromatin imaging promise to bridge the long-standing gap between sequencing studies disclosing genomic information and imaging studies providing spatial and temporal information. The project aims to harness the CRISPR-Cas9 system to develop a pioneering tool to visualize defined genomic loci in living cells of eudicot and monocot plants. see: Dreissig et al (2017). Live-cell CRISPR imaging in plants reveals dynamic telomere movements. Plant J 91, 565-573.
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If you are interested in this topic, please contact Prof. Dr. Andreas Houben for further information.