Single-molecule sequencing (single-molecule real-time SMRT sequencing): PacBio Revio (80–110 Gb sequence yield/SMRT cell) delivers long read lengths (15–20 kb) with extremely high sequencing accuracy (HiFi reads, >99.99%) in HiFi CCS mode, setting a new standard in the sequencing of large and complex plant genomes.

Its applications include de novo genome sequencing and assembly, as well as full length transcript or isoform sequencing.