© Leibniz-Institut (IPK)


Microscopy is a core technology in modern plant research. While its classical use has been to characterise morphology and structure, increasingly the focus is shifting to the study of growth and cell dynamics, where cellular and subcellular events are monitored on living plant tissue. One of leading research issues for crop scientists concerns the adaptation of plants to unfavourable environmental conditions and the strategies they adopt to cope with environmental stress. Tools developed by cell biologists and microscopists can be used to functionally characterise key structural traits, and the knowledge gained thereby can contribute to breeding efforts aimed at enhancing plant performance. Over several years, the group has been able to establish a state-of-the-art central light and electron microscopy facility, which has recently been expanded through the acquisition by the Chromosome Structure and Function Research Group of both a super-resolution light microscope and a light sheet microscope.

The research interests of the group itself are focused on the biology of grain shattering, lodging and drought stress. However, the group is also charged with the development of protocols suitable for the preparation of plant tissue destined for live cell imaging, ultrastructure analysis, three dimensional reconstruction and immunolocalisation. The combined use of the two disciplines should encourage inter-disciplinary microscopical approaches to be taken to address a range of scientific questions. In many cases, microscopy is just one component of a wider project involving other IPK or external researchers.

The central microscopy facility, which is maintained by the Structural Cell Biology Research Group, currently houses the following equipment:

Light microscopy

  • Zeiss Axio Imager M2 
  • Zeiss Axioskop
  • Zeiss Axiovert 135
  • Keyence VHX-5000
  • Zeiss LSM 510 META
  • Zeiss LSM 780
  • Andor Revolution® XD

Electron microscopy

  • Transmission Electron Microscope FEI Tecnai G2-Sphera 200 KV 
  • Field Emission Scanning Electron Microscope Zeiss Gemini300 

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The main goal of the joint project with the RG Plant Reproductive Biology and the Genetics Department at the University of Silesia is an in-depth study on ABA-SL interact ion in barley in response to drought stress. In order, to further elucidate the molecular mechanisms underlying tillering and drought tolerance, we will perform physiological, morphological and cell biological studies on barley mutant hvd14.d, and barley accessions with a natural variation in the sequence of the HvD14 gene.

Using targeted mutagenesis (CRISPR RNA / Cas9) and screening of a TILLING population, additional barley mutants with modified SL biosynthesis or the signaling pathway will be generated in order, to demonstrate direct or indirect interactions between SLs and ABA. The deep sequencing of differentially expressed genes will be complemented by an analysis of the concentration of SLs, ABA, auxins, brassinosteroids, cytokinins, gibberellins, jasmonic acid and salicylic acid with and without drought stress treatment. In addition, treatment with selected hormones, such as ABA, brassinosteroids and cytokinins of pre-selected SL mutant lines, will be used to confirm predicted interactions.

The aim of the joint project with the Satellite Collections North of the IPK Genbank, Saatzucht Steinach GmbH & Co KG and GenXPro GmbH is the development of practical selection methods for relevant genotypes that improve seed retention and thus the threshing resistance in forage and turf grasses relevant for breeding. In the Structural Cell Biology group, cell biological methods are used to develop markers for indirect trait selection before flowering and then validate them in the breeding material. The main objective was a detailed examination of the abscission zone responsible for the seed loss on the morphological and structural level. For the comparative analyzes in the various stages of development from earing to the dough stage (seed ripening), shattering-resistant and genotypes with a tendency to seed loss were used. The knowledge is intended to provide information about the point in time at which the differentiation of the abscission zone becomes visible between the genotypes and to limit the period in which genes responsible for the differentiation are active. Ultimately, the selected genotypes should improve the development of breeding lines which, in addition to the required yield and quality, have a significantly higher seed yield potential and thus make seed production much more economical than before.

Lodging resistance is an important breeding goal for rye (Secale cereale L.) because of its great height compared to other cereals. The "Stabilstroh" genotype identified in rye is characterised by its resistance to lodging, and is also characterised by increased height and biomass production compared to other German rye hybrids. To clarify the genetic background of the lodging resistance (in cooperation with IPK research groups Resource Genetics and Reproduction, RG Gene and Genome Mapping and breeding company Dieckmann Seeds GmbH), a histological and ultrastructural characterisation of the basal internodes of the parental lines and the segregating F2 population was first carried out. Using the traits identified in relation to lodging resistance, 30 genetic loci (QTLs) could then be identified on seven chromosomes. Since it is to be expected, that the trait “Stabilstroh” can also lead to increased stability in other cereals, preselected wheat and barley accessions (evaluation of historical field data from the IPK Genbank) will be examined and characterised for the development of the characteristics identified in rye. In this context, and in cooperation with the two research groups Metabolic Diversity and Resource Genetics and Reproduction, different stages of development of the parental lines of the rye genotype “Stabilstroh” are being examined in detail using histology and metabolite analysis.

In our function as the central facility for microscopy, we are also involved as a cooperation partner in the processing of other projects.

Amongst other things, we continued and intensified our close cooperation with the RG Molecular Plant Nutrition and CONICET (Rosario, Argentina) about an increased stress tolerance of plants with expression of cyanobacterial flavodoxin (Fld). We were able to show that Fld is able to replace the lost ferredoxin activity under stress conditions. Ultrastructure analysis confirmed that the expression of Fld in tobacco plants resulted also in a delay of senescence processes by regulating leaf metabolism, hormones and senescence-associated genes, and that flavodoxin-expressing tobacco plants reveal a high light acclimation-like phenotype.

Contributory work for the AG Genomics of Genetic Resources involves the transmission electron microscopic analysis of chloroplasts from a series of Arabidopsis and barley mutants as well as heterologous complementation lines. The ultrastructural data, particularly those concerning the distribution and architecture of the grana stacks, have shown to be a highly promising field of research aimed at the functional characterisation of potential genes of chloroplast biogenesis.

In cooperation with the former AG Metalloid-Transport, abnormal vascular differentiation could be described as an irreversible symptom of boron deficiency in rapeseed. Significant structural modifications especially occure in the xylem and phloem affecting cell structure and tissue organisation. The morphological alterations are much more prominent in the sensitive genotype and differ clearly from those reported for other nutrient deficiencies. To uncover genes responsible for the vascular de-differentiation under B-deficient conditions this work will be continued with Prof. Patrick Bienert from the TU Munich. Preliminary data form RNA-sequencing analysis, gene ontology analysis and the identification of differentially expressed genes suggest a re-organisation of metabolism and differentiation processes under B-deficient conditions.

In addition to a number of internal and controlled collaborations, comprehensive cell-biological studies of the development of spikelets and florets in the development stages of wheat and barley are carried out in connection with the research work of the RG Plant Architecture. Furthermore, we have embarked on producing an elaborate histological atlas on the vascular architecture of the two-row barley ear during its development. The organisation of the vasculature in the nodes, as well as between the rachis and the spikelet organs, should be visualised on the basis of sectional series and 3D models.

Several collaborations with the AG Chromosome Structure and Function focus on the ultrastructural characterisation of cell nuclei.  Using high pressure freezing  sample preparation of Aegilops speltoides in root tips , we succesfully documented differences between chromatin structure and mode of replication  between micronuclei and primary nuclei, thus supporting previous data about the precise elimination of supernumerary B chromosomes early in embryo development.

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Li M, Guo G, Pidon H, Melzer M, Prina A R, Börner T, Stein N:

ATP-dependent Clp protease subunit C1, HvClpC1, is a strong candidate gene for barley variegation mutant luteostrians as revealed by genetic mapping and genomic re-sequencing. Front. Plant Sci. 12 (2021) 664085. https://dx.doi.org/10.3389/fpls.2021.664085

Li M, Hensel G, Melzer M, Junker A, Tschiersch H, Ruwe H, Arend D, Kumlehn J, Börner T, Stein N:

Mutation of the ALBOSTRIANS ohnologous gene HvCMF3 impairs chloroplast development and thylakoid architecture in barley. Front. Plant Sci. 12 (2021) 732608. https://dx.doi.org/10.3389/fpls.2021.732608

Li M, Ruwe H, Melzer M, Junker A, Hensel G, Tschiersch H, Schwenkert S, Chamas S, Schmitz-Linneweber C, Börner T, Stein N:

The Arabidopsis AAC proteins CIL and CIA2 are sub-functionalized paralogs involved in chloroplast development. Front. Plant Sci. 12 (2021) 681375. https://dx.doi.org/10.3389/fpls.2021.681375

Muszynska A, Guendel A, Melzer M, Tandron Moya Y, Röder M, Rolletschek H, Rutten T, Munz E, Melz G, Ortleb S, Borisjuk L, Börner A:

A mechanistic view on lodging resistance in rye and wheat: a multiscale comparative study. Plant Biotechnol. J. (2021) Epub ahead of print. dx.doi.org/10.1111/pbi.13689

Thiel J, Koppolu R, Trautewig C, Hertig C, Kale S M, Erbe S, Mascher M, Himmelbach A, Rutten T, Esteban E, Pasha A, Kumlehn J, Provart N J, Vanderauwera S, Frohberg C, Schnurbusch T:

Transcriptional landscapes of floral meristems in barley. Sci. Adv. 7 (2021) eabf0832. https://dx.doi.org/10.1126/sciadv.abf0832

Vicino P, Carrillo J, Gómez R, Shahinnia F, Tula S, Melzer M, Rutten T, Carrillo N, Hajirezaei M R, Lodeyro A F:

Expression of flavodiiron proteins Flv2-Flv4 in chloroplasts of Arabidopsis and tobacco plants provides multiple stress tolerance. Int. J. Mol. Sci. 22 (2021) 1178. https://dx.doi.org/10.3390/ijms22031178

Witzel K, Matros A, Bertsch U, Aftab T, Rutten T, Ramireddy E, Melzer M, Kunze G, Mock H P:

The jacalin-related lectin HvHorcH is involved in the physiological response of barley roots to salt stress. Int. J. Mol. Sci. 22 (2021) 10248. https://dx.doi.org/10.3390/ijms221910248


Aizouq M, Peisker H, Gutbrod K, Melzer M, Hölzl G, Dörmann P:

Triacylglycerol and phytyl ester synthesis in Synechocystis sp. PCC6803. Proc. Natl. Acad. Sci. U.S.A. 117 (2020) 6216-6222. https://dx.doi.org/10.1073/pnas.1915930117

Dvořák Tomaštíková E, Rutten T, Dvořák P, Tugai A, Ptošková K, Petrovská B, van Damme D, Houben A, Doležel J, Demidov D:

Functional divergence of microtubule-associated TPX2 family members in Arabidopsis thaliana. Int. J. Mol. Sci. 21 (2020) 2183. https://dx.doi.org/10.3390/ijms21062183

Gómez R, Figueroa N, Melzer M, Hajirezaei M R, Carrillo N, Lodeyro A F:

Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype. Biochim. Biophys. Acta Bioenerg. 1861 (2020) 148211. https://dx.doi.org/10.1016/j.bbabio.2020.148211

Hertig C, Melzer M, Rutten T, Erbe S, Hensel G, Kumlehn J, Weschke W, Weber H, Thiel J:

Barley HISTIDINE KINASE 1 (HvHK1) coordinates transfer cell specification in the young endosperm. Plant J. 103 (2020) 1869-1884. https://dx.doi.org/10.1111/tpj.14875

Khosravi S, Dreissig S, Schindele P, Wolter F, Rutten T, Puchta H, Houben A:

Live Cell CRISPR imaging in plant cells with a telomere-specific guide RNA. In: Heinlein M (Ed.): RNA Tagging: methods and protocols. (Series: Methods in molecular biology, Vol. 2166) New York, NY: Humana Press (2020) 343-356. dx.doi.org/10.1007/978-1-0716-0712-1_20 1940-6029 (Electronic)1064-3745 (Linking)

Khosravi S, Schindele P, Gladilin E, Dunemann F, Rutten T, Puchta H, Houben A:

Application of aptamers improves CRISPR-based live imaging of plant telomeres. Front. Plant Sci. 11 (2020) 1254. https://dx.doi.org/10.3389/fpls.2020.01254

Le Goff S, Keçeli B N, Jeřábková H, Heckmann S, Rutten T, Cotterell S, Schubert V, Roitinger E, Mechtler K, Franklin F C H, Tatout C, Houben A, Geelen D, Probst A V, Lermontova I:

The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis. Plant J. 101 (2020) 71-86. https://dx.doi.org/10.1111/tpj.14518

Marzec M, Daszkowska-Golec A, Collin A, Melzer M, Eggert K, Szarejko I:

Barley strigolactone signaling mutant hvd14.d reveals the role of strigolactones in ABA-dependent response to drought. Plant Cell Environ. 43 (2020) 2239-2253. https://dx.doi.org/10.1111/pce.13815

Poursarebani N, Trautewig C, Melzer M, Nussbaumer T, Lundqvist U, Rutten T, Schmutzer T, Brandt R, Himmelbach A, Altschmied L, Koppolu R, Youssef H M, Sibout R, Dalmais M, Bendahmane A, Stein N, Xin Z, Schnurbusch T:

COMPOSITUM 1 contributes to the architectural simplification of barley inflorescence via meristem identity signals. Nat. Commun. 11 (2020) 5138. https://dx.doi.org/10.1038/s41467-020-18890-y

Redekop P, Rothhausen N, Rothhausen N, Melzer M, Mosebach L, Dülger E, Bovdilova A, Caffarri S, Hippler M, Jahns P:

PsbS contributes to photoprotection in Chlamydomonas reinhardtii independently of energy dissipation. Biochim. Biophys. Acta Bioenerg. 1861 (2020) 148183. https://dx.doi.org/10.1016/j.bbabio.2020.148183

Rizzo P, Altschmied L, Ravindran B M, Rutten T, DAuria J C:

The biochemical and genetic basis for the biosynthesis of bioactive compounds in Hypericum perforatum L., one of the largest medicinal crops in Europe. Genes 11 (2020) E1210. https://dx.doi.org/10.3390/genes11101210

Rolletschek H, Schwender J, König C, Chapman K D, Romsdahl T, Lorenz C, Braun H P, Denolf P, van Audenhove K, Munz E, Heinzel N, Ortleb S, Rutten T, McCorkle S, Borysyuk T, Gündel A, Shi H, Vander Auwermeulen M, Bourot S, Borisjuk L:

Cellular plasticity in response to suppression of storage proteins in the Brassica napus embryo. Plant Cell 32 (2020) 2383-2401. https://dx.doi.org/10.1105/tpc.19.00879

Ruban A, Schmutzer T, Wu D D, Fuchs J, Boudichevskaia A, Rubtsova M, Pistrick K, Melzer M, Himmelbach A, Schubert V, Scholz U, Houben A:

Supernumerary B chromosomes of Aegilops speltoides undergo precise elimination in roots early in embryo development. Nat. Commun. 11 (2020) 2764. https://dx.doi.org/10.1038/s41467-020-16594-x

Stock J, Bräutigam A, Melzer M, Bienert G P, Bunk B, Nagel M, Overmann J, Keller E R J, Mock H P:

The transcription factor WRKY22 is required during cryo-stress acclimation in Arabidopsis shoot tips. J. Exp. Bot. 71 (2020) 4993-5009. https://dx.doi.org/10.1093/jxb/eraa224

Tikhenko N, Alqudah A M, Borisjuk L, Ortleb S, Rutten T, Wu D D, Nagel M, Himmelbach A, Mascher M, Röder M, Ganal M, Sehmisch S, Houben A, Börner A:

DEFECTIVE ENDOSPERM-D1 (Dee-D1) is crucial for endosperm development in hexaploid wheat. Commun. Biol. 3 (2020) 791. https://doi.org/10.1038/s42003-020-01509-9

Tula S, Shahinnia F, Melzer M, Rutten T, Gómez R, Lodeyro A F, von Wirén N, Carrillo N, Hajirezaei M R:

Providing an additional electron sink by the introduction of cyanobacterial flavodiirons enhances the growth of Arabidopsis thaliana under various light intensities. Front. Plant Sci. 11 (2020) 902. https://dx.doi.org/10.3389/fpls.2020.00902

Wu D D, Ruban A, Rutten T, Zhou Y H, Houben A:

Analysis of pollen grains by immunostaining and FISH in Triticeae species. In: Pradillo M, Heckmann S (Eds.): Plant Meiosis: methods and protocols. (Series: Methods in molecular biology, Vol. 2061) New York, NY: Humana Press (2020) 347-358. dx.doi.org/10.1007/978-1-4939-9818-0_24 978-1-4939-9817-3


Bagheri A, Akhavan A, Abbasi S, Ashgar Maassoumi A, Rutten T, Blattner F R:

Pollen morphology of Astragalus section Hymenostegis (Fabaceae) and evaluation of its systematic implications. Grana 58 (2019) 328-336. https://dx.doi.org/10.1080/00173134.2019.1621931

Bethmann S, Melzer M, Schwarz N, Jahns P:

The zeaxanthin epoxidase is degraded along with the D1 protein during photoinhibition of photosystem II. Plant Direct 3 (2019) e00185. https://dx.doi.org/10.1002/pld3.185

Boussora F, Allam M, Guasmi F, Ferchichi A, Rutten T, Hansson M, Youssef H M, Börner A:

Spike developmental stages and ABA role in spikelet primordia abortion contribute to the final yield in barley (Hordeum vulgare L.). Bot. Stud. 60 (2019) 13. https://dx.doi.org/10.1186/s40529-019-0261-2

Demidov D, Heckmann S, Weiss O, Rutten T, Tomaštíková E D, Kuhlmann M, Scholl P, Municio C M, Lermontova I, Houben A:

Deregulated phosphorylation of CENH3 at Ser65 affects the development of floral meristems in Arabidopsis thaliana. Front. Plant Sci. 10 (2019) 928. https://dx.doi.org/10.3389/Fpls.2019.00928

Li M, Hensel G, Mascher M, Melzer M, Budhagatapalli N, Rutten T, Himmelbach A, Beier S, Korzun V, Kumlehn J, Boerner T, Stein N:

Leaf variegation and impaired chloroplast development caused by a truncated CCT domain gene in albostrians barley. Plant Cell 31 (2019) 1430-1445. https://dx.doi.org/10.1105/tpc.19.00132

Litwińska K, Bischoff F, Matthes F, Bode R, Rutten T, Kunze G:

Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticals. AMB Express 9 (2019) 102. https://dx.doi.org/10.1186/s13568-019-0832-3

Marzec M, Melzer M:

Preparation of barley roots for histological, structural, and immunolocalization studies using light and electron microscopy. In: Harwood W A (Ed.): Barley: methods and protocols. (Series: Methods in molecular biology, Vol. 1900) New York, NY: Humana Press (2019) 153-166. dx.doi.org/10.1007/978-1-4939-8944-7_10 978-1-4939-8944-7

Milner S G, Jost M, Taketa S, Mazón E R, Himmelbach A, Oppermann M, Weise S, Knüpffer H, Basterrechea M, König P, Schüler D, Sharma R, Pasam R K, Rutten T, Guo G, Xu D, Zhang J, Herren G, Müller T, Krattinger S G, Keller B, Jiang Y, González M Y, Zhao Y, Habekuß A, Färber S, Ordon F, Lange M, Börner A, Graner A, Reif J C, Scholz U, Mascher M, Stein N:

Genebank genomics highlights the diversity of a global barley collection. Nat. Genet. 51 (2019) 319-326. https://doi.org/10.1038/s41588-018-0266-x

Rizzo P, Altschmied L, Stark P, Rutten T, Guendel A, Scharfenberg S, Franke K, Baeumlein H, Wessjohann L, Koch M, Borisjuk L, Sharbel T F:

Discovery of key regulators of dark glands development and hypericin biosynthesis in St. Johns wort (Hypericum perforatum). Plant Biotechnol. J. 17 (2019) 2299-2312. https://dx.doi.org/10.1111/pbi.13141

Tedeschi F, Rizzo P, Huong B, Czihal A, Rutten T, Altschmied L, Scharfenberg S, Grosse I, Becker C, Weigel D, Bäumlein H, Kuhlmann M:

EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis. New Phytol. 221 (2019) 261-278. https://dx.doi.org/10.1111/nph.15439

Zelkowski M, Zelkowska K, Conrad U, Hesse S, Lermontova I, Marzec M, Meister A, Houben A, Schubert V:

Arabidopsis NSE4 proteins act in somatic nuclei and meiosis to ensure plant viability and fertility. Front. Plant Sci. 10 (2019) 774. https://dx.doi.org/10.3389/fpls.2019.00774


Barkla B J, Garibay-Hernández A, Melzer M, Rupasinghe T W T, Roessner U:

Single cell-type analysis of cellular lipid remodelling in response to salinity in the epidermal bladder cells of the model halophyte Mesembryanthemum crystallinum. Plant Cell Environ. 41 (2018) 2390-2403. https://dx.doi.org/10.1111/pce.13352

Döll S, Kuhlmann M, Rutten T, Mette M F, Scharfenberg S, Petridis A, Berreth D C, Mock H-P:

Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex. Plant J. 93 (2018) 431-444. https://dx.doi.org/10.1111/tpj.13797

Leijon F, Melzer M, Zhou Q, Srivastava V, Bulone V:

Proteomic analysis of plasmodesmata from Populus cell suspension cultures in relation with callose biosynthesis. Front. Plant Sci. 9 (2018) 1681. https://dx.doi.org/10.3389/fpls.2018.01681

Marzec M, Alqudah A M:

Key hormonal components regulate agronomically important traits in barley. Int. J. Mol. Sci. 19 (2018) 795. https://dx.doi.org/10.3390/ijms19030795

Marzec M, Melzer M:

Regulation of root development and architecture by strigolactones under optimal and nutrient deficiency conditions. Int. J. Mol. Sci. 19 (2018) 1887. https://dx.doi.org/10.3390/ijms19071887

Mayta M L, Lodeyro A F, Guiamet J J, Tognetti V B, Melzer M, Hajirezaei M R, Carrillo N:

Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves. Front. Plant Sci. 9 (2018) 1039. https://dx.doi.org/10.3389/fpls.2018.01039

Muszynska A:

Histological, ultrastructural, elemental, and molecular genetic characterization of ′Stabilstroh’, a complex trait of rye (Secale cereale L.) determining lodging resistance. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät III Agrar- und Ernährungswissenschaften, Geowissenschaften und Informatik (2018) 161 pp.

Rajaraman J, Douchkov D, Lück S, Hensel G, Nowara D, Pogoda M, Rutten T, Meitzel T, Brassac J, Höfle C, Hückelhoven R, Klinkenberg J, Trujillo M, Bauer E, Schmutzer T, Himmelbach A, Mascher M, Lazzari B, Stein N, Kumlehn J, Schweizer P:

Evolutionarily conserved partial gene duplication in the Triticeae tribe of grasses confers pathogen resistance. Genome Biol. 19 (2018) 116. https://dx.doi.org/10.1186/s13059-018-1472-7

Shi R, Melzer M, Zheng S, Benke A, Stich B, von Wirén N:

Iron retention in root hemicelluloses causes genotypic variability in the tolerance to iron deficiency-induced chlorosis in maize. Front. Plant Sci. 9 (2018) 557. https://dx.doi.org/10.3389/fpls.2018.00557

Witzel K, Matros A, Møller A L B, Ramireddy E, Finnie C, Peukert M, Rutten T, Herzog A, Kunze G, Melzer M, Kaspar-Schoenefeld S, Schmülling T, Svensson B, Mock H-P:

Plasma membrane proteome analysis identifies a role of barley Membrane Steroid Binding Protein in root architecture response to salinity. Plant Cell Environ. 41 (2018) 1311-1330. https://dx.doi.org/10.1111/pce.13154


Biernacki M, Marzec M, Roick T, Pätz R, Baronian K, Bode R, Kunze G:

Enhancement of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation in Arxula adeninivorans by stabilization of production. Microb. Cell Fact. 16 (2017) 144. https://dx.doi.org/10.1186/s12934-017-0751-4

Dreissig S, Rutten T, Schubert V, Houben A:

Nicotiana benthamiana. (Calendar page). In: Inaga S, Nakata M, Taniguchi K (Eds.): Chromosome Calendar 2018. Japan: The Society of Chromosome Research (2017) 1.

Dreissig S, Schiml S, Schindele P, Weiss O, Rutten T, Schubert V, Gladilin E, Mette M F, Puchta H, Houben A:

Live cell CRISPR-imaging in plants reveals dynamic telomere movements. (The Plant Journal - 2017 Best Paper Award: Gold Prize in the category Technical Advance Article). Plant J. 91 (2017) 565-573. https://dx.doi.org/10.1111/tpj.13601

Hilo A, Shahinnia F, Druege U, Franken P, Melzer M, Rutten T, von Wirén N, Hajirezaei M-R:

A specific role of iron in promoting cell division in early meristemoids during adventitious root formation. J. Exp. Bot. 68 (2017) 4233-4247. https://dx.doi.org/10.1093/jxb/erx248

Kraner M E, Link K, Melzer M, Ekici A B, Uebe S, Tarazona P, Feussner I, Hofmann J, Sonnewald U:

Choline transporter-like1 (CHER1) is crucial for plasmodesmata maturation in Arabidopsis thaliana. Plant J. 89 (2017) 394–406. https://dx.doi.org/10.1111/tpj.13393

Marzec M:

Strigolactones and gibberellins: A new couple in the phytohormone world? Trends Plant Sci. 22 (2017) 813-815. https://dx.doi.org/10.1016/j.tplants.2017.08.001

Marzec M:

Strigolactone signaling in plants. In: El-Esawi M (Ed.): Phytohormones - signaling mechanisms and crosstalk in plant development and stress responses. : IntechOpen (2017) 101-117. dx.doi.org/10.5772/intechopen.68497 ISBN 978-953-51-3411-4

Meier A, Worch S, Böer E, Hartmann A, Mascher M, Marzec M, Scholz U, Riechen J, Baronian K, Schauer F, Bode R, Kunze G:

Agdc1p – a gallic acid decarboxylase involved in the degradation of 1 tannic acid in the yeast Blastobotrys (Arxula) adeninivorans. Front. Microbiol. 8 (2017) 1777. https://dx.doi.org/10.3389/fmicb.2017.01777

Pandey P, Daghma D S, Houben A, Kumlehn J, Melzer M, Rutten T:

Dynamics of post-translationally modified histones during barley pollen embryogenesis in the presence or absence of the epi-drug trichostatin A. Plant Reprod. 30 (2017) 95-105. https://dx.doi.org/10.1007/s00497-017-0302-5

Sakuma S, Lundqvist U, Kakei Y, Thirulogachandar V, Suzuki T, Hori K, Wu J, Tagiri A, Rutten T, Koppolu R, Shimada Y, Houston K, Thomas W T B, Waugh R, Schnurbusch T, Komatsuda T:

Extreme suppression of lateral floret development by a single amino acid change in the VRS1 transcription factor. Plant Physiol. 175 (2017) 1720-1731. https://dx.doi.org/10.1104/pp.17.01149

Sandmann M, Talbert P, Demidov D, Kuhlmann M, Rutten T, Conrad U, Lermontova I:

Targeting of A. thaliana KNL2 to centromeres depends on the conserved CENPC-k motif in its C-terminus. Plant Cell 29 (2017) 144-155. https://dx.doi.org/10.1105/tpc.16.00720

Schedel S, Pencs S, Hensel G, Müller A, Rutten T, Kumlehn J:

RNA-guided Cas9-induced mutagenesis in tobacco followed by efficient genetic fixation in doubled haploid plants. Front. Plant Sci. 7 (2017) 1995. https://dx.doi.org/10.3389/fpls.2016.01995

Schlüter U, Bräutigam A, Gowik U, Melzer M, Christin P-A, Kurz S, Mettler-Altmann T, Weber A P:

Photosynthesis in C3–C4 intermediate Moricandia species. J. Exp. Bot. 68 (2017) 191-206. https://dx.doi.org/10.1093/jxb/erw391

Schumann T, Paul S, Melzer M, Dörmann P, Jahns P:

Plant growth under natural light conditions provides highly flexible short-term acclimation properties towards high light stress. Front. Plant Sci. 8 (2017) 681. https://dx.doi.org/10.3389/fpls.2017.00681

Sørensen B B, Ehrnsberger H F, Esposito S, Pfab A, Bruckmann A, Hauptmann J, Meister G, Merkl R, Schubert T, Längst G, Melzer M, Grasser M, Grasser K D:

The Arabidopsis THO/TREX component TEX1 functionally interacts with MOS11 and modulates mRNA export and alternative splicing events. Plant Mol. Biol. 93 (2017) 283–298. https://dx.doi.org/10.1007/s11103-016-0561-9

Tedeschi F, Rizzo P, Rutten T, Altschmied L, Bäumlein H:

RWP-RK domain-containing transcription factors control cell differentiation during female gametophyte development in Arabidopsis. New Phytol. 213 (2017) 1909–1924. https://dx.doi.org/10.1111/nph.14293

Thirulogachandar V, Alqudah A M, Koppolu R, Rutten T, Graner A, Hensel G, Kumlehn J, Bräutigam A, Sreenivasulu N, Schnurbusch T, Kuhlmann M:

Leaf primordium size specifies leaf width and vein number among row-type classes in barley. Plant J. 91 (2017) 601-612. https://dx.doi.org/10.1111/tpj.13590

Tikhenko N, Poursarebani N, Rutten T, Schnurbusch T, Börner A:

Embryo lethality in wheat-rye hybrids: dosage effect and deletion bin mapping of the responsible wheat locus. Biol. Plant. 61 (2017) 342-348. https://dx.doi.org/10.1007/s10535-016-0691-6

Tikhenko N, Rutten T, Senula A, Rubtsova M, Keller E R J, Börner A:

The changes in the reproductive barrier between hexaploid wheat (Triticum aestivum L.) and rye (Secale cereale L.): different states lead to different fates. Planta 246 (2017) 377–388. https://dx.doi.org/10.1007/s00425-017-2694-8

Youssef H M, Eggert K, Koppolu R, Alqudah A M, Poursarebani N, Fazeli A, Sakuma S, Tagiri A, Rutten T, Govind G, Lundqvist U, Graner A, Komatsuda T, Sreenivasulu N, Schnurbusch T:

VRS2 regulates hormone-mediated inflorescence patterning in barley. Nat. Genet. 49 (2017) 157-161. https://dx.doi.org/10.1038/ng.3717

Zhou Y, Hölzl G, vom Dorp K, Peisker H, Melzer M, Frentzen M, Dörmann P:

Identification and characterization of a plastidial phosphatylglycerophosphate phosphatase in Arabidopsis thaliana. Plant J. 89 (2017) 221–234. https://dx.doi.org/10.1111/tpj.13378


Budhagatapalli N, Schedel S, Gurushidze M, Pencs S, Hiekel S, Rutten T, Kusch S, Morbitzer R, Lahaye T, Panstruga R, Kumlehn J, Hensel G:

A simple test for the cleavage activity of customized endonucleases in plants. Plant Methods 12 (2016) 18. https://dx.doi.org/10.1186/s13007-016-0118-6

Chen W, Kastner C, Nowara D, Oliveira-Garcia E, Rutten T, Zhao Y, Deising H B, Kumlehn J, Schweizer P:

Host-induced silencing of Fusarium culmorum genes protects wheat from infection. J. Exp. Bot. 67 (2016) 4979-4991. https://dx.doi.org/10.1093/jxb/erw263

Correa-Galvis V, Poschmann G, Melzer M, Stühler K, Jahns P:

PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nat. Plants 2 (2016) 15225. https://dx.doi.org/10.1038/nplants.2015.225

Jost M, Taketa S, Mascher M, Himmelbach A, Yuo T, Shahinnia F, Rutten T, Druka A, Schmutzer T, Steuernagel B, Beier S, Taudien S, Scholz U, Morgante M, Waugh R, Stein N:

A homolog of Blade-On-Petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence. Plant Physiol. 171 (2016) 1113-1127. https://dx.doi.org/10.1104/pp.16.00124

Keller E R J, Grübe M, Hajirezaei M-R, Melzer M, Mock H-P, Rolletschek H, Senula A, Subbarayan K:

Experience in large-scale cryopreservation and links to applied research for safe storage of plant germplasm. In: Lambardi M, Hamill S (Eds.): Proceedings of the XXIX IHC - Int. Symp. on Micropropagation and In Vitro Techniques, Brisbane, Australia, August 17-22, 2014. (Series: Acta Horticulturae, Vol. 1113) Leuven: ISHS (2016) 239-249. dx.doi.org/10.17660/ActaHortic.2016.1113.36

Kelly A A, Kalisch B, Hölzl G, Schulze S, Thiele J, Melzer M, Roston R L, Benning C, Dörmann P:

Synthesis and transfer of galactolipids in the chloroplast envelope membranes of Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 113 (2016) 10714-10719. https://dx.doi.org/10.1073/pnas.1609184113

Marzec M:

Strigolactones as part of the plant defence system. Trends Plant Sci. 21 (2016) 900-903. https://dx.doi.org/10.1016/j.tplants.2016.08.010

Marzec M:

Perception and signaling of strigolactones. Front. Plant Sci. 7 (2016) 1260. https://dx.doi.org/10.3389/fpls.2016.01260

Pham H T M, Chamas A, Nieter A, Giersberg M, Rutten T, Gehrmann L, Hettwer K, Tuerk J, Uhlig S, Simon K, Baronian K, Kunze G:

Determination of glucocorticoids using photometric (A-YGS) and spectrofluorometric (A-YGFS) bioassays based on modified Arxula adeninivorans cells: applications in environmental analysis. Sensors Actuat. B 223 (2016) 540-549. https://dx.doi.org/10.1016/j.snb.2015.09.132


Batzenschlager M, Lermontova I, Schubert V, Fuchs J, Berr A, Koini M A, Houlné G, Herzog E, Rutten T, Alioua A, Fransz P, Schmit A-C, Chabouté M-E:

Arabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture. Proc. Natl. Acad. Sci. U.S.A. 112 (2015) 8656–8660. https://dx.doi.org/10.1073/pnas.1506351112

Beie I:

RKD-Transkriptionsfaktoren als zentrale Regulatoren der Gametophyt-Entwicklung bei Arabidopsis thaliana. (Master Thesis) Magdeburg, Otto-von-Guericke-Universität (2015) 61 pp.

Bohner A, Kojima S, Hajirezaei M-R, Melzer M, von Wirén N:

Urea retranslocation from senescing Arabidopsis leaves is promoted by DUR3-mediated urea retrieval from leaf apoplast. Plant J. 81 (2015) 377-387. https://dx.doi.org/10.1111/tpj.12740

Budhagatapalli N, Rutten T, Gurushidze M, Kumlehn J, Hensel G:

Targeted modification of gene function exploiting homology-directed repair of TALEN-mediated double strand breaks in barley. G3: Genes Genomes Genetics 5 (2015) 1857-1863. https://dx.doi.org/10.1534/g3.115.018762

Chittela R K, Melzer M, Sainis J K:

Comparison of activity of OsDmc1A recombinase of rice (Oryza sativa) in presence of Ca2+ and Mg2+ ions. Indian J. Biochem. Biophys. 52 (2015) 161-168.

Hensel G, Floss D M, Arcalis E, Sack M, Melnik S, Altmann F, Rutten T, Kumlehn J, Stoger E, Conrad U:

Transgenic production of an anti HIV antibody in the barley endosperm. PLoS One 10 (2015) e0140476. https://dx.doi.org/10.1371/journal.pone.0140476

Marzec M, Melzer M, Szarejko I:

Root hair development in the grasses: what we already know and what we still need to know. Plant Physiol. 168 (2015) 407-414. https://dx.doi.org/10.1104/pp.15.00158

Marzec M, Muszyńska A:

In silico analysis of the genes encoding proteins that are involved in the biosynthesis of the RMS/MAX/D pathway revealed new roles of strigolactones in plants. Int. J. Mol. Sci. 16 (2015) 6757-6782. https://dx.doi.org/10.3390/ijms16046757

Marzec M, Szarejko I, Melzer M:

Arabinogalactan proteins are involved in root hair development in barley. J. Exp. Bot. 66 (2015) 1245-1257. https://dx.doi.org/10.1093/jxb/eru475

Pandey P:

Chromatin modifications during pollen development and pollen embryogenesis in barley. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2015) 139 pp.

Pielot R, Kohl S, Manz B, Rutten T, Weier D, Tarkowská D, Rolčík J, Strnad M, Volke F, Weber H, Weschke W:

Hormone-mediated growth dynamics of the barley pericarp as revealed by magnetic resonance imaging and transcript profiling. J. Exp. Bot. 66 (2015) 6927–6943. https://dx.doi.org/10.1093/jxb/erv397

Poursarebani N, Seidensticker T, Koppolu R, Trautewig C, Gawroński P, Bini F, Govind G, Rutten T, Sakuma S, Tagiri A, Wolde G M, Youssef H M, Battal A, Ciannamea S, Fusca T, Nussbaumer T, Pozzi C, Börner A, Lundqvist U, Komatsuda T, Salvi S, Tuberosa R, Uauy C, Sreenivasulu N, Rossini L, Schnurbusch T:

The genetic basis of composite spike form in barley and Miracle-Wheat"." Genetics 201 (2015) 155-165. https://dx.doi.org/10.1534/genetics.115.176628

Rössler C:

Entwicklungsabhängige morphologische und strukturelle Analyse von Raps (Brassica napus) Sprossgeweben unter Bormangelbedingungen. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH), Fachbereich Angewandte Biowissenschaften und Prozesstechnik, Studiengang Biotechnologie (2015) 95 pp.

Schwarz N, Armbruster U, Iven T, Brückle L, Melzer M, Feussner I, Jahns P:

Tissue-specific accumulation and regulation of zeaxanthin epoxidase in Arabidopsis reflect the multiple functions of the enzyme in plastids. Plant Cell Physiol. 56 (2015) 346-357. https://dx.doi.org/10.1093/pcp/pcu167

Tikhenko N, Rutten T, Tsvetkova N, Voylokov A, Börner A:

Hybrid dwarfness in crosses between wheat (Triticum aestivum L.) and rye (Secale cereale L.): a new look at an old phenomenon. Plant Biol. 17 (2015) 320-326. https://dx.doi.org/10.1111/plb.12237


Daghma D E S, Hensel G, Rutten T, Melzer M, Kumlehn J:

Cellular dynamics during early barley pollen embryogenesis revealed by time-lapse imaging. Front. Plant Sci. 5 (2014) 675. https://dx.doi.org/10.3389/fpls.2014.00675

Demidov D, Lermontova I, Weiss O, Fuchs J, Rutten T, Kumke K, Sharbel T F, van Damme D, de Storme N, Geelen D, Houben A:

Altered expression of Aurora kinases in Arabidopsis results in aneu- and polyploidization. Plant J. 80 (2014) 449-461. https://dx.doi.org/10.1111/tpj.12647

Dürr J, Lolas I B, Sorensen B B, Schubert V, Houben A, Melzer M, Deutzmann R, Grasser M, Grasser K D:

The transcript elongation factor SPT4/SPT5 is involved in auxin-related gene expression in Arabidopsis. Nucleic Acids Res. 42 (2014) 4332-4347. https://dx.doi.org/10.1093/nar/gku096

Marzec M, Melzer M, Szarejko I:

The evolutionary context of root epidermis cell patterning in grasses (Poaceae). Plant Signal. Behav. 9 (2014) e27972. https://dx.doi.org/10.4161/psb.27972

Marzec M, Muszyńska A, Melzer M, Sas-Nowosielska H, Kurczynska E U:

Increased symplasmic permeability in barley root epidermal cells correlates with defects in root hair development. Plant Biol. 16 (2014) 476-484. https://dx.doi.org/10.1111/plb.12066

Muszyńska A, Jarocka K, Kurczynska E U:

Plasma membrane and cell wall properties of an aspen hybrid (Populus tremula x tremuloides) parenchyma cells under the influence of salt stress. Acta Physiol. Plant. 36 (2014) 1155-1165. https://dx.doi.org/10.1007/s11738-014-1490-3

Youssef H M, Koppolu R, Rutten T, Korzun V, Schweizer P, Schnurbusch T:

Genetic mapping of the labile (lab) gene: a recessive locus causing irregular spikelet fertility in labile-barley (Hordeum vulgare convar. labile). Theor. Appl. Genet. 127 (2014) 1123-1131. https://dx.doi.org/10.1007/s00122-014-2284-0


Agarwal R, Matros A, Melzer M, Mock H-P:

Blue-native page analysis validates heterogeneity in the thylakoids of Synechocystis 6803. In: Kuang T, Lu C, Zhang L (Eds.): Photosynthesis research for food, fuel and the future: 15th International Conference on Photosynthesis, Beijing, China, August 22 - 27, 2010. (Series: Advanced topics in science and technology in China) Heidelberg [u.a.]: Springer (2013) 385-388. dx.doi.org/10.1007/978-3-642-32034-7_80 ISBN 978-3-642-32033-0

Ahkami A H, Melzer M, Ghaffari M R, Pollmann S, Ghorbani Javid M, Shahinnia F, Hajirezaei M-R, Druege U:

Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation. Planta 238 (2013) 499-517. https://dx.doi.org/10.1007/s00425-013-1907-z

Blanco N E, Ceccoli R D, Vía M V, Voss I, Segretin M E, Bravo-Almonacid F F, Melzer M, Hajirezaei M-R, Scheibe R, Hanke G T:

Expression of the minor isoform pea ferredoxin in tobacco alters photosynthetic electron partitioning and enhances cyclic electron flow. Plant Physiol. 161 (2013) 866-879. https://dx.doi.org/10.1104/pp.112.211078

Harpke D, Meng S, Rutten T, Kerndorff H, Blattner F R:

Phylogeny of Crocus (Iridaceae) based on one chloroplast and two nuclear loci: ancient hybridization and chromosome number evolution. Mol. Phylogenet. Evol. 66 (2013) 617-627. https://dx.doi.org/10.1016/j.ympev.2012.10.007

Jásik J, Boggetti B, Baluška F, Volkmann D, Gensch T, Rutten T, Altmann T, Schmelzer E:

PIN2 turnover in Arabidopsis root epidermal cells explored by the photoconvertible protein Dendra2. PLoS One 8 (2013) e61403. https://dx.doi.org/10.1371/journal.pone.0061403

Kim Y M, Heinzel N, Giese J O, Koeber J, Melzer M, Rutten T, von Wirén N, Sonnewald U, Hajirezaei M-R:

A dual role of tobacco hexokinase 1 in primary metabolism and sugar sensing. Plant Cell Environ. 36 (2013) 1311-1327. https://dx.doi.org/10.1111/pce.12060

Koppolu R, Anwar N, Sakuma S, Tagiri A, Lundqvist U, Pourkheirandish M, Rutten T, Seiler C, Himmelbach A, Ariyadasa R, Youssef H M, Stein N, Sreenivasulu N, Komatsuda T, Schnurbusch T:

Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley. Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 13198-13203. https://dx.doi.org/10.1073/pnas.1221950110

Lermontova I, Kuhlmann M, Friedel S, Rutten T, Heckmann S, Sandmann M, Demidov D, Schubert V, Schubert I:

Arabidopsis KINETOCHORE NULL2 is an upstream component for cenH3 deposition at centromeres. Plant Cell 25 (2013) 3389-3404. https://dx.doi.org/10.1105/tpc.113.114736

Marzec M, Melzer M, Szarejko I:

Asymmetric growth of root epidermal cells is related to the differentiation of root hair cells in Hordeum vulgare (L.). J. Exp. Bot. 64 (2013) 5145-5155. https://dx.doi.org/10.1093/jxb/ert300

Marzec M, Muszyńska A, Gruszka D:

The role of strigolactones in nutrient-stress responses in plants. Int. J. Mol. Sci. 14 (2013) 9286-9304. https://dx.doi.org/10.3390/ijms14059286

Mau M, Corral J M, Vogel H, Melzer M, Fuchs J, Kuhlmann M, de Storme N, Geelen D, Sharbel T F:

The conserved chimeric transcript UPGRADE2 is associated with unreduced pollen formation and is exclusively found in apomictic Boechera species. Plant Physiol. 163 (2013) 1640-1659. https://dx.doi.org/10.1104/pp.113.222448

Pandey P, Houben A, Kumlehn J, Melzer M, Rutten T:

Chromatin alterations during pollen development in Hordeum vulgare. Cytogenet. Genome Res. 141 (2013) 50-57. https://dx.doi.org/10.1159/000351211

Rubtsova M, Gnad H, Melzer M, Weyen J, Gils M:

The auxins centrophenoxine and 2,4-D differ in their effects on non-directly induced chromosome doubling in anther culture of wheat (T. aestivum L.). Plant Biotechnol. Rep. 7 (2013) 247-255. https://dx.doi.org/10.1007/s11816-012-0256-x

Srivastava V, Obudulu O, Bygdell J, Löfstedt T, Rydén P, Nilsson R, Ahnlund M, Johansson A, Jonsson P, Freyhult E, Qvarnström J, Karlsson J, Melzer M, Moritz T, Trygg J, Hvidsten T R, Wingsle G:

OnPLS integration of transcriptomic, proteomic and metabolomic data shows multi-level oxidative stress responses in the cambium of transgenic hipI- superoxide dismutase Populus plants. BMC Genomics 14 (2013) 893. https://dx.doi.org/10.1186/1471-2164-14-893

Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N:

Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana. Plant J. 75 (2013) 469-483. https://dx.doi.org/10.1111/tpj.12215

Zbierzak A M, Porfirova S, Griebel T, Melzer M, Parker J E, Dörmann P:

A TIR-NBS protein encoded by Arabidopsis Chilling Sensitive 1 (CHS1) limits chloroplast damage and cell death at low temperature. Plant J. 75 (2013) 539-552. https://dx.doi.org/10.1111/tpj.12219


Agarwal R, Maralihalli G, Sudarsan V, Choudhury S D, Vatsa R K, Pal H, Melzer M, Sainis J K:

Differential distribution of pigment-protein complexes in the Thylakoid membranes of Synechocystis 6803. J. Bioenerg. Biomembr. 44 (2012) 399-409. https://dx.doi.org/10.1007/s10863-012-9437-0

Agueci F, Rutten T, Demidov D, Houben A:

Arabidopsis AtNek2 kinase is essential and associates with microtubules. Plant Mol. Biol. Rep. 30 (2012) 339-348. https://dx.doi.org/10.1007/s11105-011-0342-1

Ceccoli R D, Blanco N E, Segretin M E, Melzer M, Hanke G T, Scheibe R, Hajirezaei M-R, Bravo-Almonacid F F, Carrillo N:

Flavodoxin displays dose-dependent effects on photosynthesis and stress tolerance when expressed in transgenic tobacco plants. Planta 236 (2012) 1447-1458. https://dx.doi.org/10.1007/s00425-012-1695-x

Daghma D S, Kumlehn J, Hensel G, Rutten T, Melzer M:

Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.). J. Exp. Bot. 63 (2012) 6017-6021. https://dx.doi.org/10.1093/jxb/ers254

Hedtke B, Alawady A, Albacete A, Kobayashi K, Melzer M, Roitsch T, Masuda T, Grimm B:

Deficiency in riboflavin biosynthesis affects tetrapyrrole biosynthesis in etiolated Arabidopsis tissue. Plant Mol. Biol. 78 (2012) 77-93. https://dx.doi.org/10.1007/s11103-011-9846-1

Hensel G, Oleszczuk S, Daghma D E, Zimny J, Melzer M, Kumlehn J:

Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x Triticosecale Wittmack). BMC Plant Biol. 12 (2012) 171. https://dx.doi.org/10.1186/1471-2229-12-171

Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi T M, Renou J P, Balzergue S, Viehover P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H:

Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana. Plant J. 71 (2012) 427-442. https://dx.doi.org/10.1111/j.1365-313X.2012.04999.x

Kasaras A, Melzer M, Kunze R:

Arabidopsis senescence-associated protein DMP1 is involved in membrane remodeling of the ER and tonoplast. BMC Plant Biol. 12 (2012) 54. https://dx.doi.org/10.1186/1471-2229-12-54

Meyer R C, Witucka-Wall H, Becher M, Blacha A, Boudichevskaia A, Dörmann P, Fiehn O, Friedel S, von Korff M, Lisec J, Melzer M, Repsilber D, Schmidt R, Scholz M, Selbig J, Willmitzer L, Altmann T:

Heterosis manifestation during early Arabidopsis seedling development is characterized by intermediate gene expression and enhanced metabolic activity in the hybrids. Plant J. 71 (2012) 669-683. https://dx.doi.org/10.1111/j.1365-313X.2012.05021.x

Radchuk V, Kumlehn J, Rutten T, Sreenivasulu N, Radchuk R, Rolletschek H, Herrfurth C, Feussner I, Borisjuk L:

Fertility in barley flowers depends on Jekyll functions in male and female sporophytes. New Phytol. 194 (2012) 142-157. https://dx.doi.org/10.1111/j.1469-8137.2011.04032.x

Sedzielewska K A, Böer E, Bellebna C, Wartmann T, Bode R, Melzer M, Baronian K, Kunze G:

Role of the AFRD1 encoded fumarate reductase in hypoxia and osmotolerance in Arxula adeninivorans. FEMS Yeast Res. 12 (2012) 924-937. https://dx.doi.org/10.1111/j.1567-1364.2012.00842.x

Thiel J, Hollmann J, Rutten T, Weber H, Scholz U, Weschke W:

454 transcriptome sequencing suggests a role for two-component signalling in cellularization and differentiation of barley endosperm transfer cells. PLoS One 7 (2012) e41867. https://dx.doi.org/10.1371/journal.pone.0041867

Thiel J, Riewe D, Rutten T, Melzer M, Friedel S, Bollenbeck F, Weschke W, Weber H:

Differentiation of endosperm transfer cells of barley - a comprehensive analysis at the micro-scale. Plant J. 71 (2012) 639-655. https://dx.doi.org/10.1111/j.1365-313X.2012.05018.x


Blanco N E, Ceccoli R D, Segretin M E, Poli H O, Voss I, Melzer M, Bravo-Almonacid F F, Scheibe R, Hajirezaei M-R, Carrillo N:

Cyanobacterial flavodoxin complements ferredoxin deficiency in knocked-down transgenic tobacco plants. Plant J. 65 (2011) 922-935. https://dx.doi.org/10.1111/j.1365-313X.2010.04479.x

Czarnecki O, Hedtke B, Melzer M, Rothbart M, Richter A, Schröter Y, Pfannschmidt T, Grimm B:

An Arabidopsis GluTR binding protein mediates spatial separation of 5-aminolevulinic acid synthesis in chloroplasts. Plant Cell 23 (2011) 4476-4491. https://dx.doi.org/10.1105/tpc.111.086421

Daghma D S:

Structural changes during the initiation of pollen embryogenesis in barley (Hordeum vulgare L.). (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2011) 167 pp.

Daghma D S, Kumlehn J, Melzer M:

The use of cyanobacteria as filler in nitrocellulose capillaries improves ultrastructural preservation of immature barley pollen upon high pressure freezing. J. Microsc. 244 (2011) 79-84. https://dx.doi.org/10.1111/j.1365-2818.2011.03509.x

Karimi Ashtiyani R, Banaei Moghaddam A M, Schubert V, Rutten T, Fuchs J, Demidov D, Blattner F R, Houben A:

AtHaspin phosphorylates histone H3 at threonine 3 during mitosis and contributes to embryonic patterning in Arabidopsis. Plant J. 68 (2011) 443-454. https://dx.doi.org/10.1111/j.1365-313X.2011.04699.x

Kőszegi D, Johnston A J, Rutten T, Czihal A, Altschmied L, Kumlehn J, Wust S E, Kirioukhova O, Gheyselinck J, Grossniklaus U, Bäumlein H:

Members of the RKD transcription factor family induce an egg cell-like gene expression program. Plant J. 67 (2011) 280-291. https://dx.doi.org/10.1111/j.1365-313X.2011.04592.x

Lermontova I, Koroleva O, Rutten T, Fuchs J, Schubert V, Moraes I, Köszegi D, Schubert I:

Knockdown of CENH3 in Arabidopsis reduces mitotic divisions and causes sterility by disturbed meiotic chromosome segregation. Plant J. 68 (2011) 40-50. https://dx.doi.org/10.1111/j.1365-313X.2011.04664.x

Lermontova I, Rutten T, Schubert I:

Deposition, turnover, and release of CENH3 at Arabidopsis centromeres. Chromosoma 120 (2011) 633-640. https://dx.doi.org/10.1007/s00412-011-0338-5

Melkus G, Rolletschek H, Fuchs J, Radchuk V, Grafahrend-Belau E, Sreenivasulu N, Rutten T, Weier D, Heinzel N, Schreiber F, Altmann T, Jakob P M, Borisjuk L:

Dynamic (13) C/(1) H NMR imaging uncovers sugar allocation in the living seed. Plant Biotechnol. J. 9 (2011) 1022-1037. https://dx.doi.org/10.1111/j.1467-7652.2011.00618.x

Rudolph M, Schlereth A, Körner M, Feussner K, Berndt E, Melzer M, Hornung E, Feussner I:

The lipoxygenase-dependent oxygenation of lipid body membranes is promoted by a patatin-type phospholipase in cucumber cotyledons. J. Exp. Bot. 62 (2011) 749-760. https://dx.doi.org/10.1093/Jxb/Erq310

Tschiersch H, Borisjuk L, Rutten T, Rolletschek H:

Gradients of seed photosynthesis and its role for oxygen balancing. Biosystems 103 (2011) 302-308. https://dx.doi.org/10.1016/j.biosystems.2010.08.007


Agarwal R, Matros A, Melzer M, Mock H-P, Sainis J K:

Heterogeneity in thylakoid membrane proteome of Synechocystis 6803. J. Proteomics 73 (2010) 976-991. https://dx.doi.org/10.1016/j.jprot.2009.12.011

Arsova B, Hoja U, Wimmelbacher M, Greiner E, Üstün S, Melzer M, Petersen K, Lein W, Börnke F:

Plastidial thioredoxin z interacts with two fructokinase-like Proteins in a thiol-dependent manner: Evidence for an essential role in chloroplast development in Arabidopsis and Nicotiana benthamiana. Plant Cell 22 (2010) 1498-1515. https://dx.doi.org/10.1105/tpc.109.071001

Johnston A J, Kirioukhova O, Barrell P J, Rutten T, Moore J M, Baskar R, Grossniklaus U, Gruissem W:

Dosage-sensitive function of RETINOBLASTOMA RELATED and convergent epigenetic control are required during the Arabidopsis life cycle. PLoS Genet. 6 (2010) e1000988. https://dx.doi.org/10.1371/journal.pgen.1000988

Kotseruba V, Pistrick K, Blattner F R, Kumke K, Weiss O, Rutten T, Fuchs J, Endo T, Nasuda S, Ghukasyan A, Houben A:

The evolution of the hexaploid grass Zingeria kochii (Mez) Tzvel. (2n=12) was accompanied by complex hybridization and uniparental loss of ribosomal DNA. Mol. Phylogenet. Evol. 56 (2010) 146-155. https://dx.doi.org/10.1016/j.ympev.2010.01.003

Lolas I B, Himanen K, Grønlund J T, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser K D:

The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2. Plant J. 61 (2010) 686-697. https://dx.doi.org/10.1111/j.1365-313X.2009.04096.x

Wiedemann G, Hermsen C, Melzer M, Buttner-Mainik A, Rennenberg H, Reski R, Kopriva S:

Targeted knock-out of a gene encoding sulfite reductase in the moss Physcomitrella patens affects gametophytic and sporophytic development. FEBS Lett. 584 (2010) 2271-2278. https://dx.doi.org/10.1016/j.febslet.2010.03.034


Agarwal R, Ortleb S, Sainis J K, Melzer M:

Immunoelectron microscopy for locating calvin cycle enzymes in the thylakoids of Synechocystis 6803. Mol. Plant 2 (2009) 32-42. https://dx.doi.org/10.1093/mp/ssn075

Ahkami A H, Lischewski S, Haensch K T, Porfirova S, Hofmann J, Rolletschek H, Melzer M, Franken P, Hause B, Druege U, Hajirezaei M-R:

Molecular physiology of adventitious root formation in Petunia hybrida cuttings: involvement of wound response and primary metabolism. New Phytol. 181 (2009) 613-625. https://dx.doi.org/10.1111/j.1469-8137.2008.02704.x

Chang C C, Slesak I, Jorda L, Sotnikov A, Melzer M, Miszalski Z, Mullineaux P M, Parker J E, Karpinska B, Karpinski S:

Arabidopsis chloroplastic glutathione peroxidases play a role in cross-talk between photooxidative stress and immune responses. Plant Physiol. 150 (2009) 670-683. https://dx.doi.org/10.1104/pp.109.135566

Demidov D, Hesse S, Tewes A, Rutten T, Fuchs J, Ashtiyani R K, Lein S, Fischer A, Reuter G, Houben A:

Aurora1 phosphorylation activity on histone H3 and its cross-talk with other post-translational histone modifications in Arabidopsis. Plant J. 59 (2009) 221-230. https://dx.doi.org/10.1111/j.1365-313X.2009.03861.x

Grasser M, Kane C M, Merkle T, Melzer M, Emmersen J, Grasser K D:

Transcript elongation factor TFIIS is involved in Arabidopsis seed dormancy. J. Mol. Biol. 386 (2009) 598-611. https://dx.doi.org/10.1016/j.jmb.2008.12.066

Hölzl G, Witt S, Gaude N, Melzer M, Schöttler M A, Dörmann P:

The role of diglycosyl lipids in photosynthesis and membrane lipid homeostasis in Arabidopsis. Plant Physiol. 150 (2009) 1147-1159. https://dx.doi.org/10.1104/pp.109.139758

Lippmann R, Kaspar S, Rutten T, Melzer M, Kumlehn J, Matros A, Mock H-P:

Protein and metabolite analysis reveals permanent induction of stress defense and cell regeneration processes in a tobacco cell suspension culture. Int. J. Mol. Sci. 10 (2009) 3012-3032. https://dx.doi.org/10.3390/ijms10073012

Melkus G, Rolletschek H, Radchuk R, Fuchs J, Rutten T, Wobus U, Altmann T, Jakob P, Borisjuk L:

The metabolic role of the legume endosperm: a noninvasive imaging study. Plant Physiol. 151 (2009) 1139-1154. https://dx.doi.org/10.1104/pp.109.143974

Srivastava V, Srivastava M K, Chibani K, Nilsson R, Rouhier N, Melzer M, Wingsle G:

Alternative splicing studies of the reactive oxygen species gene network in Populus reveal two isoforms of high-isoelectric-point superoxide dismutase. Plant Physiol. 149 (2009) 1848-1859. https://dx.doi.org/10.1104/pp.108.133371

Van Son L, Tiedemann J, Rutten T, Hillmer S, Hinz G, Zank T, Manteuffel R, Bäumlein H:

The BURP domain protein AtUSPL1 of Arabidopsis thaliana is destined to the protein storage vacuoles and overexpression of the cognate gene distorts seed development. Plant Mol. Biol. 71 (2009) 319-329. https://dx.doi.org/10.1007/s11103-009-9526-6

Weigelt K, Küster H, Rutten T, Fait A, Fernie A R, Miersch O, Wasternack C, Emery R J, Desel C, Hosein F, Müller M, Saalbach I, Weber H:

ADP-glucose pyrophosphorylase-deficient Pea embryos reveal specific transcriptional and metabolic changes of carbon-nitrogen metabolism and stress responses. Plant Physiol. 149 (2009) 395-411. https://dx.doi.org/10.1104/pp.108.129940

Zurbriggen M D, Carrillo N, Tognetti V B, Melzer M, Peisker M, Hause B, Hajirezaei M-R:

Chloroplast-generated reactive oxygen species play a major role in localized cell death during the non-host interaction between tobacco and Xanthomonas campestris pv. vesicatoria. Plant J. 60 (2009) 962-973. https://dx.doi.org/10.1111/j.1365-313X.2009.04010.x


Biesenbaum W, Rutten T:

Nachweis von Ectodemia amani SVENSSON, 1966 in Sachsen-Anhalt und Baden-Würtemberg (Lep., Nepticulidae). Melangaria 20 (2008) 48-51.

Ivanov R, Tiedemann J, Czihal A, Schallau A, Diep L, Mock H-P, Claus B, Tewes A, Bäumlein H:

EFFECTOR OF TRANSCRIPTION2 is involved in xylem differentiation and includes a functional DNA single strand cutting domain. Dev. Biol. 313 (2008) 93-106. https://dx.doi.org/10.1016/j.ydbio.2007.09.061

Kabanov D S, Ivanov A Y, Melzer M, Prokhorenko I R:

Effects of surface proteins of human erythrocyte membrane on the interaction with lipopolysaccharides from Escherichia coli O55:B5. Biochem. (Moscow) Suppl. Ser. A: Membr. Cell Biol. 2 (2008) 117-121. https://dx.doi.org/10.1134/S1990747808020050

Kaczmarczyk A, Rutten T, Melzer M, Keller E R:

Ultrastructural changes associated with cryopreservation of potato (Solanum tuberosum l.) shoot tips. Cryo Lett. 29 (2008) 145-156.

Leroch M, Neuhaus H E, Kirchberger S, Zimmermann S, Melzer M, Gerhold J, Tjaden J:

Identification of a novel adenine nucleotide transporter in the endoplasmic reticulum of Arabidopsis. Plant Cell 20 (2008) 438-451. https://dx.doi.org/10.1105/tpc.107.057554

Neuberger T, Sreenivasulu N, Rokitta M, Rolletschek H, Göbel C, Rutten T, Radchuk V, Feussner I, Wobus U, Jakob P, Webb A, Borisjuk L:

Quantitative imaging of oil storage in developing crop seeds. Plant Biotechnol. J. 6 (2008) 31-45. https://dx.doi.org/10.1111/j.1467-7652.2007.00294.x

Rajanikant C, Melzer M, Rao B J, Sainis J K:

Homologous recombination properties of OsRad51, a recombinase from rice. Plant Mol. Biol. 68 (2008) 479–491. https://dx.doi.org/10.1007/s11103-008-9385-6

Thiel J, Weier D, Sreenivasulu N, Strickert M, Weichert N, Melzer M, Czauderna T, Wobus U, Weber H, Weschke W:

Different hormonal regulation of cellular differentiation and function in nucellar projection and endosperm transfer cells - a microdissection-based transcriptome study of young barley grains. Plant Physiol. 148 (2008) 1436-1452. https://dx.doi.org/10.1104/pp.108.127001

Tiedemann J, Rutten T, Mönke G, Vorwieger A, Rolletschek H, Meissner D, Milkowski C, Petereck S, Mock H-P, Zank T, Bäumlein H:

Dissection of a complex seed phenotype: novel insights of FUSCA3 regulated developmental processes. Dev. Biol. 317 (2008) 1-12. https://dx.doi.org/10.1016/j.ydbio.2008.01.034

Tikhenko N, Rutten T, Voylokov A, Houben A:

Analysis of hybrid lethality in F-1 wheat-rye hybrid embryos. Euphytica 159 (2008) 367-375. https://dx.doi.org/10.1007/s10681-007-9528-x


Gernand D, Golczyk H, Rutten T, Ilnicki T, Houben A, Joachimiak A J:

Tissue culture triggers chromosome alterations, amplification, and transposition of repeat sequences in Allium fistulosum. Genome 50 (2007) 435-442. https://dx.doi.org/10.1139/G07-023

Kronberg K, Vogel F, Rutten T, Hajirezaei M-R, Sonnewald U, Hofius D:

The silver lining of a viral agent: increasing seed yield and harvest index in Arabidopsis by ectopic expression of the potato leaf roll virus movement protein. Plant Physiol. 145 (2007) 905-918. https://dx.doi.org/10.1104/pp.107.102806

Ortleb S:

Untersuchungen zur Architektur des Photosyntheseapparates von Synechococcus sp. PCC 7942 und Synechocystis sp. PCC 6803 (Cyanobacteria: Chroococcales). (Diploma Thesis) Köthen, Hochschule Anhalt (2007) 88 pp.

Reinhold T, Alawady A, Grimm B, Beran K C, Jahns P, Conrath U, Bauer J, Reiser J, Melzer M, Jeblick W, Neuhaus H E:

Limitation of nocturnal import of ATP into Arabidopsis chloroplasts leads to photooxidative damage. Plant J. 50 (2007) 293-304. https://dx.doi.org/10.1111/j.1365-313X.2007.03049.x

Rolletschek H, Nguyen T H, Häusler R E, Rutten T, Göbel C, Feussner I, Radchuk R, Tewes A, Claus B, Klukas C, Linemann U, Weber H, Wobus U, Borisjuk L:

Antisense inhibition of the plastidial glucose-6-phosphate/phosphate translocator in Vicia seeds shifts cellular differentiation and promotes protein storage. Plant J. 51 (2007) 468-484. https://dx.doi.org/10.1111/j.1365-313X.2007.03155.x

Srivastava V, Schinkel H, Witzell J, Hertzberg M, Torp M, Srivastava M K, Karpinska B, Melzer M, Wingsle G:

Downregulation of high-isoelectric-point extracellular superoxide dismutase mediates alterations in the metabolism of reactive oxygen species and developmental disturbances in hybrid aspen. Plant J. 49 (2007) 135-148. https://dx.doi.org/10.1111/j.1365-313X.2006.02943.x

Voigt M-L, Melzer M, Rutten T, Mitchell-Olds T, Sharbel T F:

Gametogenesis in the apomictic Boechera holboellii complex: the male perspective. In: Hörandl E, Grossniklaus U, Sharbel T F (Eds.): Apomixis: evolution, mechanisms and perspectives. (Series: Regnum vegetabile, Vol. 147) Rugell, Liechtenstein: Gantner (2007) 235-257. ISBN 978-3-906166-60-5


Fritsch R M, Kruse J, Adler K, Rutten T:

Testa sculptures in Allium L. subg. Melanocrommyum (Webb & Berth.) Rouy (Alliaceae). Feddes Repert. 117 (2006) 250-263. https://dx.doi.org/10.1002/fedr.200611094

Gernand D, Rutten T, Pickering R, Houben A:

Elimination of chromosomes in Hordeum vulgare x H. bulbosum crosses at mitosis and interphase involves micronucleus formation and progressive heterochromatinization. Cytogenet. Genome Res. 114 (2006) 169-174. https://dx.doi.org/10.1159/000093334

Müller F, Houben A, Barker P E, Xiao Y, Käs J A, Melzer M:

Quantum dots - a versatile tool in plant science? J. Nanobiotechnol. 4 (2006) 5. https://dx.doi.org/10.1186/1477-3155-4-5

Ortleb S:

Charakterisierung der molekularen Architektur des Photosyntheseapparates bei Cyanobakterien. (Diploma Thesis) Köthen, Hochschule Anhalt (FH) (2006)

Tognetti V B, Palatnik J F, Fillat M F, Melzer M, Hajirezaei M-R, Valle E M, Carrillo N:

Functional replacement of ferredoxin by a cyanobacterial flavodoxin in tobacco confers broad-range stress tolerance. Plant Cell 18 (2006) 2035-2050. https://dx.doi.org/10.1105/tpc.106.042424

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