Single-molecule real-time SMRT sequencing

Single-molecule sequencing

Single-molecule real-time sequencing is provided by a PACBIO Sequel II (300-400 Gbp sequence output/SMRT cell; CLR and CCS) device able to deliver 15-20 kbp high accuracy (>99.99%) reads when run in CCS mode. Its applications include de novo genome sequencing and assembly, as well as full length transcript or isoform sequencing.