Yeast Genetics

Research fields

Head: Dr. FalkoMatthes (komm.) , Prof. Dr. Gotthard Kunze

The main field of research in the yeast genetics unit is the non-conventional, non-pathogen yeast Arxula adeninivorans and its use for the production of recombinant proteins, as a gene-donor and biocatalyst for new biotechnological products. It is also utilised as the microbial component in biosensors for the analysis of waste water, urine and blood, for the monitoring of feed and food and for medical investigations.

The yeast A. adeninivorans was used already in the 1980's for the production of single cell protein in Malchin (Mecklenburg-Western Pomerania) because of its rapid growth, extraordinarily high cell titers and its braod substrate spectrum. Parallel to the biotechnological utilisation investigations took place regarding its taxonomical classification and its physiology. In cooperation with the department yeast genetics of the University of Greifswald molecular tools were developed for the production of mutants by chemical mutagenesis, protoplast fusion and mitotic segregation as the basis for further improvement of A. adeninivorans as host for the production of new biotechnological products and the construction, establishment and validation of microbial biosensors. Such biosensors for the monitoring of food and environmental conditions as well as medical and pharmaceutical purposes were developed starting from the 1990's in the working group yeast genetics under the direction of Professor Dr. habil. Gotthard Kunze.

The genome of A. adeninivorans

Beginning in the early 1990's the genome of A. adeninivorans was analysed in detail by DNA reassociation studies. At the same time physiological studies were accomplished concerning usable substrates as carbon and nitrogen sources and the corresponding metabolic pathways were examined. In this context the first transgenic A. adeninivorans strains were constructed. In the following years several A. adeninivorans genes and its 25S rDNA were isolated and characterised leading to establishment of a transformation/expression system for the production of recombinant proteins in transgenic yeast strains. This system called Xplor®2 was further developed to a modular platform (expression modules, selection marker modules, ARS modules, Chaperon modules) for fast and effective construction of desired plasmids. In order to make this platform usable for the construction of custom-made transgenic production strains with high acceptance, it was established in close cooperation with small and medium-sized enterprises (KMUs) like the ARTES Biotechnology GmbH in Langenfeld. The main advantage of this patent-registered platform is that only essential DNA sequences are transformed avoiding undesired E. coli sequences and resistance genes. Since the expression modules stably integrate in the A. adeninivorans genome the transformed yeast strains show a high mitotic stability, essential for biotechnological application. 

In 2010 the complete genome of A. adeninivorans was sequenced and annotated at the IPK making the Xplor®2 system even more attractive.

Development of sensors for environmental survey

Due to the ease of genetic manipulation of A. adeninivorans several collaborations have been established especially with KMUs. Among other things A. adeninivorans cells were tested for the first time as microbial components of biosensors for the measurement of the biochemical oxygen demand (BOD). With this system the BOD in waste water, tap water, brackish water and sea water was determined within 70 seconds demonstrating the usability of the yeast for pollution control of aqueous samples.

During the last years we have focused on Arxula based biosensors for application to environmental survey, in the agriculture and for medical purposes. In several collaborations with industrial partners, research facilities and federal offices numerous biosensors were established for the determination of hormones, dioxin and pharmaceuticals in tap water, soda water, waste water, urine and blood serum. The used transgenic A. adeninivorans cells contain the genetic information for the constitutive expression of the particular human receptor which interacts with the appropriate ligand (estrogen, androgen, progesteron, glucocorticoids, dioxin, pharmaceuticals) resulting in expression of a reporter gene which encodes a recombinant enzyme detectable by simple biochemical or amperometrical measurements. Since the activity of the enzyme correlates directly with the concentration of the hormone or pharmaceutical, all substances interacting with the appropriate receptor can be measured as so-called sum parameters. First assays for the detection of estrogenic and androgenic activities in tap water, soda water and waste water were already commercialised by the industrial partner. Assays for the determination of estrogens, androgens, progesterone, glucocorticoids, dioxins and pharmaceuticals in waste water, urine and blood serum are in the phase of validation and expected to enter the market in 2012/13.

Development of sensors for medical purpose

Apart from the application in biosensors A. adeninivorans is an excellent host for the production of recombinant proteins like above mentioned human receptors. However, since some recombinant proteins require a different host to be synthesised in a functional manner, the Xplor®2 transformation/expression platform was further developed. Now additional yeasts like Hansenula polymorpha can be transformed and exploited as producers of recombinant proteins. The first functional recombinant receptors (e.g. human estrogen receptor α and human progesterone receptor) are synthesised at present for application in environmental analytics and, furthermore, in medical examinations. In the near future a surface plasmon resonance (SPR) platform for cancer analysis (e.g. breast cancer-interaction of Herceptin with the receptor HER2 [new]) and testing of novel drug-receptor-interactions will be developed in collaboration with the Fraunhofer Institute and industrial partners. 

Additional fields of application for transgenic yeasts

In parallel to the above described applications transgenic yeasts, preferably A. adeninivorans, have been and will be designed for the following biotechnological applications: 

  1. Recombinant tannase enzyme as admixture of animal feeds and/or as additive for the increase of the yield in biogas plants.
  2. Production of food with decreased purine content using an enzymatic procedure.
  3. Use of A. adeninivorans as biocatalyst for the production of n-butanol.
  4. Processing and recuperation of metals from tailings and waste dump material as well as for the processing of poor ores.
  5. Use of A. adeninivorans as microbial component of the fuel cell.
  1.  

Weinkontaminanten Vor-Ort

Nachweis von Weinkontaminanten Vor-Ort per Multiplex-Wein-Stick bzw. im Labor per Multiplex-Makrodot-Assay.  

Ziel eines von der Investitionsbank Sachsen-Anhalt geförderten Vorhabens ist die Entwicklung neuer innovativer biologischer Testsysteme zum schnellen und sensitiven Nachweis von Weinkontaminanten. Hierzu werden durch Übertragung des biologischen Nachweissystems (DNA-RNA-Hybridisierung) auf einen Multiplex-Wein-Stick bzw. Multiplex-Makrodot-Assay im Mikrotiterplattenformat Systeme zum schnellen, sensitiven und eindeutigen Nachweis von Weinkontaminanten für die Weinindustrie etabliert und validiert. Der direkt für Vor-Ort Analysen ausgelegte Multiplex-Wein-Stick soll zukünktig in einer Ja/Nein-Aussage und semi-quantitativen Detektion dem Winzer einen ersten Überblick über die Qualität seines Produktes hinsichtlich Kontamination geben. Bei einer ja-Aussage kan anschließend der für das Labor ausgelegter Multiplex-Makrodot-Assay dieser Plattform als Bestätigungsverfahren zusätzlich.

Publications

Author
Title
2023

Wegner U, Matthes F, von Wirén N, Hajirezaei M-R, Bode R, Kunze G, Rauter M:

A transaminase with ß-activity from Variovorax boronicumulans for the production of enantiopure ß-amino acids. Heliyon 9 (2023) e12729. https://dx.doi.org/10.1016/j.heliyon.2022.e12729

Wegner U, Matthes F, von Wirén N, Lemke I, Bode R, Vorbrodt H-M, Rauter M, Kunze G:

Enhancing a Sphaerobacter thermophilus ω-transaminase for kinetic resolution of β- and γ-amino acids. AMB Express 13 (2023) 117. https://dx.doi.org/10.1186/s13568-023-01623-x

2022

Meier A, Worch S, Hartmann A, Marzec M, Mock H-P, Bode R, Kunze G, Matthes F:

Characterization of catechol-1,2-dioxygenase (Acdo1p) from Blastobotrys raffinosifermentans and investigation of its role in the catabolism of aromatic compounds. Front. Microbiol. 13 (2022) 872298. https://dx.doi.org/10.3389/fmicb.2022.872298

Nietz D, Bode R, Kunze G, Rauter M:

A new lipase (Alip2) with high potential for enzymatic hydrolysis of the diester diethyladipate to the monoester monoethyladipate. Enzyme Microb. Technol. 153 (2022) 109898. https://dx.doi.org/10.1016/j.enzmictec.2021.109898

Rauter M, Nietz D, Kunze G:

Cutinase ACut2 from Blastobotrys raffinosifermentans for the selective desymmetrization of the symmetric diester diethyl adipate to the monoester monoethyl adipate. Microorganisms 10 (2022) 1316. https://dx.doi.org/10.3390/microorganisms10071316

2021

Witzel K, Matros A, Bertsch U, Aftab T, Rutten T, Ramireddy E, Melzer M, Kunze G, Mock H-P:

The jacalin-related lectin HvHorcH is involved in the physiological response of barley roots to salt stress. Int. J. Mol. Sci. 22 (2021) 10248. https://dx.doi.org/10.3390/ijms221910248

2020

Anu, Kumar A, Rapoport A, Kunze G, Kumar S, Singh D, Singh B:

Multifarious pretreatment strategies for the lignocellulosic substrates for the generation of renewable and sustainable biofuels: A review. Renewable Energy 160 (2020) 1228-1252. https://doi.org/10.1016/j.renene.2020.07.031

Litwińska K:

Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in enzymatic removal of pharmaceuticals from wastewater. (PhD Thesis) Greifswald, Universität Greifswald, Mathematisch-Naturwissenschaftliche Fakultät (2020) 131 pp.

Sanya D R A, Onesime D, Kunze G, Neuveglise C, Le Coq A C:

The native acyltransferase-coding genes DGA1 and DGA2 affect lipid accumulation in Blastobotrys raffinosifermentans differently when overexpressed. FEMS Yeast Res. 20 (2020) foaa060. https://dx.doi.org/10.1093/femsyr/foaa060

Schubert R, Werner S, Cirka H, Rödel P, Tandron Moya Y, Mock H P, Hutter I, Kunze G, Hause B:

Effects of arbuscular mycorrhization on fruit quality in industrialized tomato production. Int. J. Mol. Sci. 21 (2020) 7029. https://dx.doi.org/10.3390/ijms21197029

Zühlke M K, Schlüter R, Mikolasch A, Henning A K, Giersberg M, Lalk M, Kunze G, Schweder T, Urich T, Schauer F:

Biotransformation of bisphenol A analogues by the biphenyl-degrading bacterium Cupriavidus basilensis – a structure-biotransformation relationship. Appl. Microbiol. Biotechnol. 104 (2020) 3569-3583. https://dx.doi.org/10.1007/s00253-020-10406-4

2019

Biernacki M:

Microbial production of Poly(3-hydroxybutyrate-3-hydroxyvalerate)-copolymers from starch-containing byproducts. (PhD Thesis) Greifswald, Universität Greifswald, Mathematisch-Naturwissenschaftliche Fakultät (2019) 122 pp.

Bischoff F, Giersberg M, Matthes F, Schwalenberg T, Worch S, Kunze G:

Selection of the optimal host for the synthesis of recombinant enzymes. In: Gasser B, Mattanovich D (Eds.): Recombinant protein production in yeasts. (Series: Methods in molecular biology, Vol. 1923) New York, NY: Humana Press (2019) ISBN 978-1-4939-9023-8, 113-132. https://dx.doi.org/10.1007/978-1-4939-9024-5

Jähne M, Giersberg M:

Messung von Bisphenolen im Spurenbereich mit einem Ready-to-use-Hefezell-Assay. LABO 11 (2019) www.labo.de.

Litwińska K, Bischoff F, Matthes F, Bode R, Rutten T, Kunze G:

Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticals. AMB Express 9 (2019) 102. https://dx.doi.org/10.1186/s13568-019-0832-3

Liu D, Keiblinger K M, Leitner S, Wegner U, Zimmermann M, Fuchs S, Lassek C, Riedel K, Zechmeister-Boltenstern S:

Response of microbial communities and their metabolic functions to drying(-)rewetting stress in a temperate forest soil. Microorganisms 7 (2019) 129. https://dx.doi.org/10.3390/microorganisms7050129

Meier A K:

Identification and characterisation of gallic acid decarboxylase (Agdc1p) and catechol-1,2-dioxygenase (Acdo1p) and their role in the degradation of Tannic Acid in the Yeast Blastobotrys (Arxula) adeninivorans. (PhD Thesis) Greifswald, Universität Greifswald, Mathematisch-Naturwissenschaftliche Fakultät (2019) 168 pp.

Thomas S, Sanya D R A, Fouchard F, Nguyen H V, Kunze G, Neuveglise C, Crutz-Le Coq A M:

Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars. Biotechnol. Biofuels 12 (2019) 154. https://dx.doi.org/10.1186/s13068-019-1492-x

2018

Brückner C, Oreb M, Kunze G, Boles E, Tripp J:

An expanded enzyme toolbox for production of cis, cis-muconic acid and other shikimate pathway derivatives in Saccharomyces cerevisiae. FEMS Yeast Res. 18 (2018) foy017. https://dx.doi.org/10.1093/femsyr/foy017

Gehrmann L, Bielak H, Behr M, Itzel F, Lyko S, Simon A, Kunze G, Dopp E, Wagner M, Tuerk J:

(Anti-)estrogenic and (anti-)androgenic effects in wastewater during advanced treatment: comparison of three in vitro bioassays. Environ. Sci. Pollut. Res. 25 (2018) 4094-4104. https://dx.doi.org/10.1007/s11356-016-7165-4

Hardelt M:

Etablierung eines zellfreien Testsystems auf Basis von DNA-RNA Hybridisierung im Mikrotiterplattenformat. (Bachelor Thesis) Köthen, Hochschule Anhalt, Studiengang Biotechnologie (2018) 54 pp.

Hettwer K, Jähne M, Frost K, Giersberg M, Kunze G, Trimborn M, Reif M, Türk J, Gehrmann L, Dardenne F, De Croock F, Abraham M, Schoop A, Waniek J J, Bucher T, Simon E, Vermeirssen E, Werner A, Hellauer K, Wallentits U, Drewes J E, Dietzmann D, Routledge E, Beresford N, Zietek T, Siebler M, Simon A, Bielak H, Hollert H, Müller Y, Harff M, Schiwy S, Simon K, Uhlig S:

Validation of Arxula Yeast Estrogen Screen assay for detection of estrogenic activity in water samples: Results of an international interlaboratory study. Sci. Total. Environ. 621 (2018) 612-625. https://doi.org/10.1016/j.scitotenv.2017.11.211

Kottwitz A K:

Gezielte Mutagenese zur Konstruktion funktionaler Varianten einer bakteriellen HMF-Oxidase in Arxula adeninivorans. (Bachelor Thesis) Köthen, Hochschule Anhalt, Studiengang Biotechnologie (2018) 63 pp.

Kutschan K J:

Identifikation und Charakterisierung von rekombinanten Antikörpern gegen pflanzliche Reserveproteine. (Bachelor Thesis) Köthen, Hochschule Anhalt, Studiengang Biotechnologie (2018) 84 pp.

Ott V:

Expression pharmazeutisch relevanter Proteine in der Grünalge Chlamydomonas rheinhardtii. (Bachelor Thesis) Köthen, Hochschule Anhalt, Studiengang Biotechnologie (2018) 84 pp.

Witzel K, Matros A, Møller A L B, Ramireddy E, Finnie C, Peukert M, Rutten T, Herzog A, Kunze G, Melzer M, Kaspar-Schoenefeld S, Schmülling T, Svensson B, Mock H-P:

Plasma membrane proteome analysis identifies a role of barley Membrane Steroid Binding Protein in root architecture response to salinity. Plant Cell Environ. 41 (2018) 1311-1330. https://dx.doi.org/10.1111/pce.13154

2017

Biernacki M, Marzec M, Roick T, Pätz R, Baronian K, Bode R, Kunze G:

Enhancement of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation in Arxula adeninivorans by stabilization of production. Microb. Cell Fact. 16 (2017) 144. https://dx.doi.org/10.1186/s12934-017-0751-4

Biernacki M, Riechen J, Hähnel U, Roick T, Baronian K, Bode R, Kunze G:

Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans. AMB Express 7 (2017) 4. https://dx.doi.org/10.1186/s13568-016-0303-z

Bischoff F:

Identifizierung und Charakterisierung dreier Cutinasen von Arxula adeninivorans und deren Einsatz zum Abbau von Polyestern. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2017) 152 pp.

Bischoff F, Chamas A, Litwińska K, Matthes F, Böer E, Kunze G:

Applications of Blastobotrys (Arxula) adeninivorans in biotechnology. In: Satyanarayana T, Kunze G (Eds.): Yeast diversity for human welfare. Singapore: Springer Singapore (2017) 978-981-10-2621-8, 455-479. https://dx.doi.org/10.1007/978-981-10-2621-8_18

Bohndorf A:

Nutzung der Protoplastenfusion zur Herstellung höherploider Pelargonium crispum-Hybriden (P.× crispum). (Master Thesis) Köthen, Hochschule Anhalt, Studiengang Biotechnologie (2017)

Chamas A, Pham H T, Jähne M, Hettwer K, Uhlig S, Simon K, Einspanier A, Baronian K, Kunze G:

Simultaneous detection of three sex steroid hormone classes using a novel yeast-based biosensor. Biotechnol. Bioeng. 114 (2017) 1539–1549. https://dx.doi.org/10.1002/bit.26249

Chamas A, Pham H T M, Baronian K, Kunze G:

Biosensors based on yeast/fungal cells. In: Sibirny A A (Ed.): Biotechnology of yeasts and filamentous fungi. Cham: Springer (2017) ISBN: 978-3-319-58829-2, 351-371. https://dx.doi.org/10.1007/978-3-319-58829-2_12

Chamas A, Pham H T M, Jähne M, Hettwer K, Gehrmann L, Tuerk J, Uhlig S, Simon K, Baronian K, Kunze G:

Separation and identification of hormone-active compounds using a combination of chromatographic separation and yeast-based reporter assay. Sci. Total. Environ. 605-606 (2017) 507-513. https://dx.doi.org/10.1016/j.scitotenv.2017.06.077

Meier A, Worch S, Böer E, Hartmann A, Mascher M, Marzec M, Scholz U, Riechen J, Baronian K, Schauer F, Bode R, Kunze G:

Agdc1p – a gallic acid decarboxylase involved in the degradation of 1 tannic acid in the yeast Blastobotrys (Arxula) adeninivorans. Front. Microbiol. 8 (2017) 1777. https://dx.doi.org/10.3389/fmicb.2017.01777

Reso J:

Untersuchungen zur rekombinanten Expression und Charakterisierung von bakteriellen Cellulosom-Komponenten in der Hefe Arxula adeninivorans. (Bachelor Thesis) Köthen, Hochschule Anhalt, Studiengang Biotechnologie (2017)

Satyanarayana T, Kunze G (Eds.):

Yeast diversity for human welfare. Singapore: Springer (2017) ISBN 978-981-10-2620-1, XIV, 486 pp. https://dx.doi.org/10.1007/978-981-10-2621-8

Williams J, Trautwein-Schult A, Kunze G, Baronian K:

Environmental and metabolic parameters affecting the uric acid production of Arxula adeninivorans. Appl. Microbiol. Biotechnol. 101 (2017) 4725-4736. https://dx.doi.org/10.1007/s00253-017-8199-3

Worch S, Lemke I:

Gene expression analysis in Arxula adeninivorans: a nested quantitative real time PRC approach. In: Satyanarayana T, Kunze G (Eds.): Yeast diversity for human welfare. Singapore: Springer (2017) ISBN 978-981-10-2620-1, 251-256.

Zühlke M-K, Schlüter R, Mikolasch A, Zühlke D, Giersberg M, Schindler H, Henning A-K, Frenzel H, Hammer E, Lalk M, Bornscheuer U T, Riedel K, Kunze G, Schauer F:

Biotransformation and reduction of estrogenicity of bisphenol A by the biphenyl-degrading Cupriavidus basilensis. Appl. Microbiol. Biotechnol. 101 (2017) 3743-3758. https://dx.doi.org/10.1007/s00253-016-8061-z

2016

Faber M:

Charakterisierung zweier Rapskultivare auf Bormangeltoleranz durch Metabolit-Element- und BOR-Transportprotein-Analysen. (Bachelor Thesis) Köthen, Hochschule Anhalt (2016) 104 pp.

Kasprzak J:

Alcohol dehydrogenases as biocatalysts for the production of enantiomerically pure chiral alcohols. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2016) 141 pp.

Kasprzak J, Bischoff F, Rauter M, Becker K, Baronian K, Bode R, Schauer F, Vorbrodt H M, Kunze G:

Synthesis of 1-(S)-phenylethanol and ethyl (R)-4-chloro-3-hydroxybutanoate using recombinant Rhodococcus erythropolis alcohol dehydrogenase produced by two yeast species. Biochem. Eng. J. 106 (2016) 107-117. https://dx.doi.org/10.1016/j.bej.2015.11.007

Kasprzak J, Rauter M, Denter S, Becker K, Baronian K, Bode R, Schauer F, Piontek M, Vorbrodt H M, Kunze G:

Synthesis of ethyl (R)-mandelate using recombinant Carboxydothermus hydrogenoformans alcohol dehydrogenase produced by two yeast species. J. Mol. Catalysis B-Enzymatic 133 (2016) 176-186. https://dx.doi.org/10.1016/j.molcatb.2016.08.012

Kasprzak J, Rauter M, Riechen J, Worch S, Baronian K, Bode R, Schauer F, Kunze G:

Characterization of an Arxula adeninivorans alcohol dehydrogenase involved in the metabolism of ethanol and 1-butanol. FEMS Yeast Res. 16 (2016) fow018. https://dx.doi.org/10.1093/femsyr/fow018

Malak A, Baronian K, Kunze G:

Blastobotrys (Arxula) adeninivorans – a promising alternative yeast for biotechnology and basic research. Yeast 33 (2016) 535-547. https://dx.doi.org/10.1002/yea.3180

Matiebe A:

Genomweite Identifizierung von Bortransportern und Charakterisierung der Bor-Aufnahmekinetik in zwei Raps (Brassica napus) Kultivaren, die sich in ihrer Bormangel-Toleranz unterscheiden. (Bachelor Thesis) Köthen, Hochschule Anhalt (2016) 66 pp.

Müller J:

Rekombinante Expression und biochemische Charakterisierung von drei 2,3-Butandiol-Dehydrogenasen aus Arxula adeninivorans und Klebsiella pneumoniae. (Bachelor Thesis) Köthen, Hochschule Anhalt (2016) 56 pp.

Pham H T M, Chamas A, Nieter A, Giersberg M, Rutten T, Gehrmann L, Hettwer K, Tuerk J, Uhlig S, Simon K, Baronian K, Kunze G:

Determination of glucocorticoids using photometric (A-YGS) and spectrofluorometric (A-YGFS) bioassays based on modified Arxula adeninivorans cells: applications in environmental analysis. Sensors Actuat. B 223 (2016) 540-549. https://dx.doi.org/10.1016/j.snb.2015.09.132

Rauter M, Kasprzak J, Becker K, Riechen J, Worch S, Hartmann A, Mascher M, Scholz U, Baronian K, Bode R, Schauer F, Matthias Vorbrodt H, Kunze G:

Aadh2p: an Arxula adeninivorans alcohol dehydrogenase involved in the first step of the 1-butanol degradation pathway. Microb. Cell Fact. 15 (2016) 175. https://dx.doi.org/10.1186/s12934-016-0573-9

Schatz J:

Arbeiten zur Adaption des bakteriellen Cohesin-Dockerin-Systems in Hefen. (Bachelor Thesis) Köthen, Hochschule Anhalt (2016) 51 pp.

Zabek A, Swierkot J, Malak A, Zawadzka I, Deja S, Bogunia-Kubik K, Mlynarz P:

Application of 1H NMR-based serum metabolomic studies for monitoring female patients with rheumatoid arthritis. J. Pharm. Biomed. Anal. 117 (2016) 544-550. https://dx.doi.org/10.1016/j.jpba.2015.10.007

Zühlke M-K, Schlüter R, Henning A-K, Lipka M, Mikolasch A, Schumann P, Giersberg M, Kunze G, Schauer F:

A novel mechanism of conjugate formation of bisphenol A and its analogues by Bacillus amyloliquefaciens: Detoxification and reduction of estrogenicity of bisphenols. Int. Biodeter. Biodegr. 109 (2016) 165-173. https://dx.doi.org/10.1016/j.ibiod.2016.01.019

2015

Bischoff F, Litwińska K, Cordes A, Baronian K, Bode R, Schauer F, Kunze G:

Three new cutinases from the yeast Arxula adeninivorans that are suitable for biotechnological applications. Appl. Environ. Microbiol. 81 (2015) 5497-5510. https://dx.doi.org/10.1128/AEM.00894-15

Bohndorf A:

In-vitro-Polyploidisierung bei Pelargonium x crispum. (Bachelor Thesis) Köthen, Hochschule Anhalt, Studiengang Biotechnologie (2015)

Chamas A:

Developmment of novel Surface Plasmon Resonance-based biosensors with purified recombinant human HER-2 and progesterone receptor produced in two different yeast species. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2015) 151 pp.

Chamas A, Giersberg M, Friedrich K, Sonntag F, Kunze D, Uhlig S, Simon K, Baronian K, Kunze G:

Purification and immunodetection of the complete recombinant HER-2[neu] receptor produced in yeast. Protein Expres. Purif. 105 (2015) 61-70. https://dx.doi.org/10.1016/j.pep.2014.10.004

Chamas A, Nieter A, Pham H T, Giersberg M, Hettwer K, Uhlig S, Simon K, Baronian K, Kunze G:

Development of a recombinant Arxula adeninivorans cell bioassay for the detection of molecules with progesterone activity in wastewater. Anal. Bioanal. Chem. 407 (2015) 8109-8120. https://dx.doi.org/10.1007/s00216-015-8985-7

Gerlach T:

Entwicklung neuer Testsysteme zur Erfassung östrogener und androgener Wirkungen im Rahmen der Umweltanalytik. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2015) 134 pp.

Hofmann U:

Konstruktion einer Biokomponente zur Detektion Estrogen-, Androgen- und Glucocorticoid-wirksamer Substanzen, sowie Untersuchung dieser auf ihre Wirksamkeit. (Master Thesis) Köthen, Hochschule Anhalt (FH) (2015) 71 pp.

Jankowska D A, Trautwein-Schult A, Cordes A, Bode R, Baronian K, Kunze G:

A novel enzymatic approach in the production of food with low purine content using Arxula adeninivorans endogenous and recombinant purine degradative enzymes. Bioengineered 6 (2015) 20-25. https://dx.doi.org/10.4161/21655979.2014.991667

Kumari A, Baronian K, Kunze G, Gupta R:

Extracellular expression of YlLip11 with a native signal peptide from Yarrowia lipolytica MSR80 in three different yeast hosts. Protein Expres. Purif. 110 (2015) 138-144. https://dx.doi.org/10.1016/j.pep.2015.02.016

Pasitka L:

Gentechnische Modifikationen zur Charakterisierung von Butandioldehydrogenasen in Arxula adeninivorans. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2015) 47 pp.

Pham H T M, Giersberg M, Gehrmann L, Hettwer K, Tuerk J, Uhlig S, Hanke G, Weisswange P, Simon K, Baronian K, Kunze G:

The determination of pharmaceuticals in wastewater using a recombinant Arxula adeninivorans whole cell biosensor. Sensors Actuat. B 211 (2015) 439-448. https://dx.doi.org/10.1016/j.snb.2015.01.107

Rauter M:

Herstellung rekombinanter Alkoholdehydrogennasen in der Hefe Arxula adeninivorans und ihre Verwendung für die Synthese enantiomerenreiner Alkohole. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität, Mathematisch-Naturwissenschaftliche Fakultät (2015) 114 pp.

Rauter M, Prokoph A, Kasprzak J, Becker K, Baronian K, Bode R, Kunze G, Vorbrodt H:

Coexpression of Lactobacillus brevis ADH with GDH or G6PDH in Arxula adeninivorans for the synthesis of 1-(R)-phenylethanol. Appl. Microbiol. Biotechnol. 99 (2015) 4723-4733. https://dx.doi.org/10.1007/s00253-014-6297-z

Stolpe S:

Klonierung, Expression und Charakterisierung der Aldehyddehydrogenasen Aald2p und Aadld5p aus Arxula adeninivorans. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2015) 63 pp.

Vijayan V, Giersberg M, Chamas A, Mehrotra M, Chelikani V, Kunze G, Baronian K:

Use of recombinant oestrogen binding protein for the electrochemical detection of oestrogen. Biosens. Bioelectron. 66 (2015) 379-384. https://dx.doi.org/10.1016/j.bios.2014.11.043

2014

Cordes A, Kunze G, Trautwein-Schult A, Jankowska D, Hoferichter P, Klein C:

Verfahren zum enzymatischen Purinabbau (Patent). Veröffentlichung: 27.11.2014, IPK-Nr.2012/04. DE 10 2013 008 875.5 (2014).

Gerlach T, Knaust J, Kaiser C, Körner M, Hettwer K, Uhlig S, Simon K, Baronian K, Kunze G:

Development and assessment of a novel Arxula adeninivorans androgen screen (A-YAS) assay and its application in analysis of cattle urine. Sci. Total Environ. 490 (2014) 1073-1081. https://dx.doi.org/10.1016/j.scitotenv.2014.05.100

Giersberg M, Kunze G:

Verbindung und Verfahren zum Nachweis von Analyten (Patent), Veröffentlichung: 17.07.2014, IPK-Nr. 2012/02. DE102013207146.9 (2014).

Jankowska D:

Development of an enzyme-based method for production og food with low purine content. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2014) 145 pp.

Jansen J:

Annotation phenolischer Metabolite mittels Isotopenmarkierung und massenspektrometrischer Techniken in Blattextrakten der Zuckerrübe. (Bachelor Thesis) Köthen, Hochschule Anhalt (2014) 54 pp.

Kunze G, Gaillardin C, Czernicka M, Durrens P, Martin T, Böer E, Gabaldón T, Cruz J A, Talla E, Marck C, Goffeau A, Barbe V, Baret P, Baronian K, Beier S, Bleykasten C, Bode R, Casaregola S, Despons L, Fairhead C, Giersberg M, Gierski P P, Hähnel U, Hartmann A, Jankowska D, Jubin C, Jung P, Lafontaine I, Leh-Louis V, Lemaire M, Marcet-Houben M, Mascher M, Morel G, Richard G-F, Riechen J, Sacerdot C, Sarkar A, Savel G, Schacherer J, Sherman D J, Stein N, Straub M-L, Thierry A, Trautwein-Schult A, Vacherie B, Westhof E, Worch S, Dujon B, Souciet J-L, Wincker P, Scholz U, Neuvéglise C:

The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest. Biotechnol. Biofuels 7 (2014) 66. https://dx.doi.org/10.1186/1754-6834-7-66

Leupelt A:

Rekombinante Expression und biochemische Charakterisierung zweier Xylosidasen aus Arxula adeninivorans. (Bachelor Thesis) Köthen, Hochschule Anhalt (2014) 49 pp.

Lüpken T, Stein N, Perovic D, Habekuß A, Serfling A, Krämer I, Hähnel U, Steuernagel B, Scholz U, Ariyadasa R, Martis M M, Mayer K F X, Niks R E, Collins N C, Friedt W, Ordon F:

High resolution mapping of the barley Ryd3 locus controlling tolerance to BYDV. Mol. Breed. 33 (2014) 477-488. https://dx.doi.org/10.1007/s11032-013-9966-1

Möring J P:

Expression von humanen Hormonrezeptoren in Hansenula polymorpha sowie Untersuchung von Ligand Rezeptorwechselwirkungen mittels SPR-Verfahren. (Bachelor Thesis) Köthen, Hochschule Anhalt (2014) 87 pp.

Rauter M, Kasprzak J, Becker K, Baronian K, Bode R, Kunze G, Vorbrodt H M:

ADH from Rhodococcus ruber expressed in Arxula adeninivorans for the synthesis of 1-(S)-phenylethanol. J. Mol. Catalysis B-Enzymatic 104 (2014) 8-16. https://dx.doi.org/10.1016/j.molcatb.2014.02.015

Rauter M, Kasprzak J, Denter S, Becker K, Baronian K, Bode R, Kunze G, Vorbrodt H M:

Reusability of ADH and GDH producing Arxula adeninivorans cells and cell extract for the production of 1-(S)-phenylethanol. J. Mol. Catalysis B-Enzymatic 108 (2014) 72-76. https://dx.doi.org/10.1016/j.molcatb.2014.06.008

Sebald C:

Artenbestimmung von autochshonen arbuskulären Mykorrhizapilzen mittels RFLP-Analyse. (Bachelor Thesis) Köthen, Hochschule Anhalt (2014) 60 pp.

Stöckmann C, Palmen T G, Schroer K, Kunze G, Gellissen G, Büchs J:

Definition of culture conditions for Arxula adeninivorans, a rational basis for studying heterologous gene expression in this dimorphic yeast. J. Ind. Microbiol. Biotechnol. 41 (2014) 965-976. https://dx.doi.org/10.1007/s10295-014-1433-4

Trautwein-Schult A, Jankowska D, Cordes A, Hoferichter P, Klein C, Matros A, Mock H-P, Baronian K, Bode R, Kunze G:

Arxula adeninivorans recombinant guanine deaminase and its application in the production of food with low purine content. J. Mol. Microbiol. Biotechnol. 24 (2014) 67-81. https://dx.doi.org/10.1159/000357674

Williams J, Trautwein-Schult A, Jankowska D, Kunze G, Squire M A, Baronian K:

Identification of uric acid as the redox molecule secreted by the yeast Arxula adeninivorans. Appl. Microbiol. Biotechnol. 98 (2014) 2223-2229. https://dx.doi.org/10.1007/s00253-013-5487-4

Zielke M K:

Abdh2p – eine Butandioldehydrogenase für die Synthese von 2,3 Butandiol. (Bachelor Thesis) Köthen, Hochschule Anhalt (2014) 70 pp.

2013

Álvaro-Benito M, Fernández-Lobato M, Baronian K, Kunze G:

Assessment of Schwanniomyces occidentalis as a host for protein production using the wide-range Xplor®2 expression platform. Appl. Microbiol. Biotechnol. 97 (2013) 4443-4456. https://dx.doi.org/10.1007/s00253-012-4527-9

Behrens A-K:

Modellierung und Simulation des Purin-Abbaus in der Hefe Arxula adeninivorans. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH), Fachbereich Angewandte Biowissenschaften und Prozesstechnik (2013) 69 pp.

Florschütz K, Schröter A, Schmieder S, Chen W, Schweizer P, Sonntag F, Danz N, Baronian K, Kunze G:

Phytochip: on-chip detection of phytopathogenic RNA viruses by a new surface plasmon resonance platform. J. Virol. Methods 189 (2013) 80-86. https://dx.doi.org/10.1016/j.jviromet.2013.01.008

Jankowska D A, Faulwasser K, Trautwein-Schult A, Cordes A, Hoferichter P, Klein C, Bode R, Baronian K, Kunze G:

Arxula adeninivorans recombinant adenine deaminase and its application in the production of food with low purine content. J. Appl. Microbiol. 115 (2013) 1134-1146. https://dx.doi.org/10.1111/jam.12317

Jankowska D A, Trautwein-Schult A, Cordes A, Hoferichter P, Klein C, Bode R, Baronian K, Kunze G:

Arxula adeninivorans xanthine oxidoreductase and its application in the production of food with low purine content. J. Appl. Microbiol. 115 (2013) 796-807. https://dx.doi.org/10.1111/jam.12284

Kalla K:

Die Untersuchung der Biosorption von Seltenen Erden an biologischen Komponenten. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2013) 85 pp.

Kremp J:

Artenbestimmung von autochthonen arbuskulären Mykorrhizapilzen. (Bachelor Thesis) Furtwangen, Hochschule Furtwangen (FH) (2013) 42 pp.

Lüpken T, Stein N, Perovic D, Habekuss A, Kramer I, Hähnel U, Steuernagel B, Scholz U, Zhou R, Ariyadasa R, Taudien S, Platzer M, Martis M, Mayer K, Friedt W, Ordon F:

Genomics-based high-resolution mapping of the BaMMV/BaYMV resistance gene rym11 in barley (Hordeum vulgare L.). Theor. Appl. Genet. 126 (2013) 1201-1212. https://dx.doi.org/10.1007/s00122-013-2047-3

Pham T M H, Kunath K, Gehrmann L, Giersberg M, Tuerk J, Uhlig S, Hanke G T, Simon K, Baronian K, Kunze G:

Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater samples. Sensor. Actuat. B 185 (2013) 628-637. https://dx.doi.org/10.1016/j.snb.2013.05.065

Prokoph A:

Lactobacillus brevis - Alkoholdehydrogenase als Biokatalysator zur Herstellung von enantiomeren reinen Alkoholen. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2013) 84 pp.

Rauter M, Schwarz M, Becker K, Baronian K, Bode R, Kunze G, Vorbrodt H M:

Synthesis of benzyl β-D-galactopyranoside by transgalactosylation using a β-galactosidase produced by the over-expression of the Kluyveromyces lactis LAC4 gene in Arxula adeninivorans. J. Mol. Catalysis B Enzymatic 97 (2013) 319-327. https://dx.doi.org/10.1016/j.molcatb.2013.06.017

Richter V:

Rekombinante Expression des hCAR-Gens in Escherichia coli und Hansenula polymorpha. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2013) 63 pp.

Trautwein-Schult A:

Purinarme Lebensmittel – Enzymatische Reduktion von Purinen in Lebensmitteln. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2013) 136 pp.

Trautwein-Schult A, Jankowska D, Cordes A, Hoferichter P, Klein C, Matros A, Mock H-P, Baronian K, Bode R, Kunze G:

Arxula adeninivorans recombinant urate oxidase and its application in the production of food with low uric acid content. J. Mol. Microbiol. Biotechnol. 23 (2013) 418-430. https://dx.doi.org/10.1159/000353847

Zoll T:

Biotechnologische Stammoptimierung zur Steigerung der Syntheseleistung von n-Butanol in Arxula adeninivorans. (Bachelor Thesis) Mittweida, Hochschule Mittweida (FH) (2013) 50 pp.

2012

Borrero J, Kunze G, Jiménez J J, Böer E, Gútiez L, Herranz C, Cintas L M, Hernández P E:

Cloning, production and functional expression of the bacteriocin enterocin A, produced by Enterococcus faecium T136, by the yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha and Arxula adeninivorans. Appl. Environ. Microbiol. 78 (2012) 5956-5961. https://dx.doi.org/10.1128/AEM.00530-12

Chelikani V, Downard A J, Kunze G, Gooneratne R, Pasco N, Baronian K H R:

Investigating yeast cell responses to oestrogen by electrochemical detection. Electrochim. Acta 73 (2012) 136-140. https://dx.doi.org/10.1016/j.electacta.2011.11.078

Gehrmann L, Pham T M H, Portner C, Giersberg M, Kunze G, Türk J:

Arxula adeninivorans basierende Detektionssysteme zur summarischen Bestimmung von estrogen aktiven Substanzen in Kläranlagenabläufen. In: Gesellschaft Deutscher Chemiker - Fachgruppe Wasserchemische Gesellschaft (Ed.): Wasser 2012 - Jahrestagung der Wasserchemischen Gesellschaft, 14.-16. Mai 2012 in Neu-Ulm: Kurzreferate. Frankfurt am Main: Gesellschaft Dt. Chemiker (2012) ISBN 978-3-936028-71-3, 234-237.

Giersberg M, Degelmann A, Bode R, Piontek M, Kunze G:

Production of a thermostable alcohol dehydrogenase from Rhodococcus ruber in three different yeast species using the Xplor®2 transformation/expression platform. J. Ind. Microbiol. Biotechnol. 39 (2012) 1385-1396. https://dx.doi.org/10.1007/s10295-012-1134-9

Giersberg M, Florschütz K, Baronian K, Kunze G:

Arxula adeninivorans (Blastobotrys adeninivorans) – an imperfect dimorphic yeast of biotechnological potential. In: Satyanarayana T, Johri B, Prakash A (Eds.): Microorganisms in sustainable agriculture and biotechnology. Dordrecht [u.a.]: Springer (2012) ISBN 978-94-007-2213-2, 453-468. https://dx.doi.org/10.1007/978-94-007-2214-9_21

Giersberg M, Florschütz K, Baronian K, Kunze G:

Wide Range Yeast Expression System ‘Xplor (R)’ – an optimized transformation/expression system for recombinant protein production in yeasts. In: Tiwari S K, Singh B (Eds.): Current trends in biotechnology: principles and applications. Saarbrücken: Lambert Academic Publishing (2012) ISBN 978-3-659-15773-8, 3-26.

Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi T M, Renou J P, Balzergue S, Viehover P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H:

Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana. Plant J. 71 (2012) 427-442. https://dx.doi.org/10.1111/j.1365-313X.2012.04999.x

Mönke G, Seifert M, Keilwagen J, Mohr M, Grosse I, Hähnel U, Junker A, Weisshaar B, Conrad U, Bäumlein H, Altschmied L:

Toward the identification and regulation of the Arabidopsis thaliana ABI3 regulon. Nucleic Acids Res. 40 (2012) 8240-8254. https://dx.doi.org/10.1093/nar/gks594

Nieter A:

Untersuchungen zum Ligandenbindungsverhalten des humanen Progesteronrezeptors B im heterologen System Hefe. (Master Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2012) 98 pp.

Pham Thi M H:

Amperometric detection of hormonal activity by a yeast cell biosensor. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2012) 143 pp.

Pham T M H, Giersberg M, Uhlig S, Hanke G, Simon K, Kunath K, Baronian K, Kunze G:

EstraMonitor – a monitor for amperometric detection of estrogenic activity with Arxula adeninivorans yeast cells as the biocomponent. Sensors Actuators B: Chemical 161 (2012) 137-145. https://dx.doi.org/10.1016/j.snb.2011.09.085

Portner C, Gehrmann L, Wirth F, Pham Thi M H, Giersberg M, Türk J, Kunze G:

Photometric and amperometric determination of estrogen activity in wastewater samples using Arxula adeninivorans yeast cell systems. 23. Internationale Fachmesse für Instrumentelle Analytik, Labortechnik und Biotechnologie (Analytica) mit analytica conference, München, 2012 (2012)

Saß J:

Hefen als mikrobielle Biosensoren für den Nachweis von Pharmazeutika. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2012) 59 pp.

Sędzielewska K A:

A molecular approach to characterize arbuscular mycorrhizal fungus, Glomus sp. AMykor isolate. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2012) 114 pp.

Sedzielewska K A, Böer E, Bellebna C, Wartmann T, Bode R, Melzer M, Baronian K, Kunze G:

Role of the AFRD1 encoded fumarate reductase in hypoxia and osmotolerance in Arxula adeninivorans. FEMS Yeast Res. 12 (2012) 924-937. https://dx.doi.org/10.1111/j.1567-1364.2012.00842.x

Sedzielewska K A, Vetter K, Bode R, Baronian K, Watzke R, Kunze G:

GiFRD encodes a protein involved in anaerobic growth in the arbuscular mycorrhizal fungus Glomus intraradices. Fungal Genet. Biol. 49 (2012) 313-321. https://dx.doi.org./10.1016/j.fgb.2012.02.002

2011

Böer E, Breuer F S, Weniger M, Denter S, Piontek M, Kunze G:

Large-scale production of tannase using the yeast Arxula adeninivorans. Appl. Microbiol. Biotechnol. 92 (2011) 105-114. https://dx.doi.org/10.1007/s00253-011-3320-5

Chelikani V, Rawson F J, Downard A J, Gooneratne R, Kunze G, Pasco N, Baronian K H R:

Electrochemical detection of oestrogen binding protein interaction with oestrogen in Candida albicans cell lysate. Biosens. Bioelectron. 26 (2011) 3737-3741. https://dx.doi.org/10.1016/j.bios.2011.01.016

Dähne M:

Untersuchungen des HOG-Pathways bei Hefen. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2011) 59 pp.

Florschütz K, Sonntag F, Schröter A, Schmieder S, Klotzbach U, Kunze G:

On-Chip-Nachweis von phytopathogenen RNA-Viren und miRNA mittels Oberflächenplasmonenresonanz. In: Sensorforschung für Medizin und Technik - Ergebnisse aus Projekten der industriellen Gemeinschaftsforschung, Sensor und Test 2011, Juni 2011, Nürnberg. (2011) 35-40.

Gerlach T, Cherkouk C, Rebohle L, Howitz S, Hanke G, Lehmann P, Jähne M, Simon K, Uhlig S, Kunze G:

Rezeptor-selektive Anreicherung und Bio-LED-Sensorik zur qualitativen und quantitativen Detektion von östrogen- und androgen-wirkenden Substanzen. In: Institut für Bioprozeß- und Analysenmeßtechnik, Gesellschaft Deutscher Chemiker, Arbeitskreis Chemo- und Biosensoren (Eds.): Programm - DBS 2011, 7. Deutsches BioSensor Symposium: 3. - 6. April 2011, Heilbad Heiligenstadt, abstracts. Heilbad Heiligenstadt (2011) ISBN 978-3-00-034073-4, 58.

Giese K:

Tanninabbau in Arxula adeninivorans – Charakterisierung und Funktionsanalyse der Tannase 2 (Atan2p). (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2011) 104 pp.

Greif H:

Nachweis der Pregnan-X-(PXR)-, Retinoid-X-(RXR)-Rezeptor Heterodimerisierung durch bimolekulare Fluoreszenz-Komplementation. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2011) 83 pp.

Haslett N D, Rawson F J, Barriere F, Kunze G, Pasco N, Gooneratne R, Baronian K H R:

Characterisation of yeast microbial fuel cell with the yeast Arxula adeninivorans as the biocatalyst. Biosens. Bioelectron. 26 (2011) 3742-3747. https://dx.doi.org/10.1016/j.bios.2011.02.011

Kahlo A:

Enzyme als Biokatalysatoren zur Synthese von Zuckerderivaten für die Biomedizin und Biotechnologie. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2011) 77 pp.

Sêdzielewska K A, Fuchs J, Temsch E M, Baronian K, Watzke R, Kunze G:

Estimation of the Glomus intraradices nuclear DNA content. New Phytol. 192 (2011) 794-797. https://dx.doi.org/10.1111/j.1469-8137.2011.03937.x

Singh B, Kunze G, Satyanarayana T:

Developments in biochemical aspects and biotechnological applications of microbial phytases. Biotechnol. Mol. Biol. Rev. 6 (2011) 69-87.

Worch S, Kalladan R, Harshavardhan V T, Pietsch C, Korzun V, Kuntze L, Börner A, Wobus U, Röder M S, Sreenivasulu N:

Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality. BMC Plant Biol. 11 (2011) 1. https://dx.doi.org/10.1186/1471-2229-11-1

2010

Florschütz K, Schröter A, Körner M, Schmieder S, Sonntag F, Vetter K, Watzke R, Kunze G:

Auf den Spuren von Pilzen und Viren mittels SPR und Plattenassay. In: Beckmann D, Institut für Bioprozeß- und Analysenmeßtechnik (Eds.): Technische Systeme für die Lebenswissenschaften: 15. Heiligenstädter Kolloquium, 27. – 29.09. 2010. Heilbad Heiligenstadt: Institut für Bioprozeß- und Analysenmeßtechnik (2010) ISBN 978-3-00-032249-5, 185-192.

Giersberg M, Floss D M, Kipriyanov S, Conrad U, Scheller J:

Covalent dimerization of camelidae anti-human TNF-alpha single domain antibodies by the constant kappa light chain domain improves neutralizing activity. Biotechnol. Bioeng. 106 (2010) 161-166. https://dx.doi.org/10.1002/bit.22653

Kaiser C:

Etablierung eines auf Hefezellen basierenden Assays zur Erfassung östrogener Wirkungen im Rahmen der Umwelt-, Nahrungs- und Futtermittelanalytik. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2010) 175 pp.

Kaiser C, Uhlig S, Gerlach T, Körner M, Simon K, Kunath K, Florschütz K, Baronian K, Kunze G:

Evaluation and validation of a novel Arxula adeninivorans estrogen screen (nAES) assay and its application in analysis of wastewater, seawater, brackish water and urine. Sci. Total Environ. 408 (2010) 6017-6026. https://dx.doi.org/10.1016/j.scitotenv.2010.08.050

Kaur P, Singh B, Böer E, Straube N, Piontek M, Satyanarayana T, Kunze G:

Pphy-A cell-bound phytase from the yeast Pichia anomala: Molecular cloning of the gene PPHY and characterization of the recombinant enzyme. J. Biotechnol. 149 (2010) 8-15. https://dx.doi.org/10.1016/j.jbiotec.2010.06.017

Klemann D:

Genetic diversity of arbuscular mycorrhizal fungi (AMP) and the influence of AMF on salt tolerance in Medicago truncatula. (Master Thesis) Hohenheim, Universität Hohenheim (2010) 98 pp.

Knabben I, Regestein L, Grumbach C, Steinbusch S, Kunze G, Büchs J:

Online determination of viable biomass up to very high cell densities in Arxula adeninivorans fermentations using an impedance signal. J. Biotechnol. 149 (2010) 60-66. https://dx.doi.org/10.1016/j.jbiotec.2010.06.007

Molitor R:

Isolation und Charakterisierung der aus Arxula adeninivorans stammenden Gallussäure-Dekarboxylase (GSDC)- und Dihydroxybenzoate-Decarboxylase (ADBC)-Gene. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2010) 56 pp.

Nieter A:

Entwicklung eines auf Hefezellen basierenden Biosensors zum Nachweis von Substanzen mit glucocorticoider Wirkung. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2010) 49 pp.

Radchuk R, Conrad U, Saalbach I, Giersberg M, Emery R J, Küster H, Nunes-Nesi A, Fernie A R, Weschke W, Weber H:

Abscisic acid deficiency of developing pea embryos achieved by immunomodulation attenuates developmental phase transition and storage metabolism. Plant J. 64 (2010) 715-730. https://dx.doi.org/10.1111/j.1365-313X.2010.04376.x

Sietmann R, Uebe R, Böer E, Bode R, Kunze G, Schauer F:

Novel metabolic routes during the oxidation of hydroxylated aromatic acids by the yeast Arxula adeninivorans. J. Appl. Microbiol. 108 (2010) 789-799. https://dx.doi.org/10.1111/j.1365-2672.2009.04474.x

Witzel K, Weidner A, Surabhi G K, Varshney R K, Kunze G, Buck-Sorlin G H, Börner A, Mock H-P:

Comparative analysis of the grain proteome fraction in barley genotypes with contrasting salinity tolerance during germination. Plant Cell Environ. 33 (2010) 211-222. https://dx.doi.org/10.1111/j.1365-3040.2009.02071.x

2009

Böer E, Bode R, Mock H-P, Piontek M, Kunze G:

Atan1p - an extracellular tannase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme. Yeast 26 (2009) 323-337. https://dx.doi.org/10.1002/yea.1669

Böer E, El Metabteb G, El Fiki A, Brückner P, Wartmann T, Piontek M, Kunze G:

The MAPk ASTE11 is involved in the maintenance of cell wall integrity and in filamentation in Arxula adeninivorans, but not in adaptation to hypertonic stress. FEMS Yeast Res. 9 (2009) 468-477. https://dx.doi.org/10.1111/j.1567-1364.2009.00496.x

Böer E, Piontek M, Kunze G:

Xplor(R) 2 - an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans. Appl. Microbiol. Biotechnol. 84 (2009) 583-594. https://dx.doi.org/10.1007/s00253-009-2167-5

Böer E, Schröter A, Bode R, Piontek M, Kunze G:

Characterization and expression analysis of a gene cluster for nitrate assimilation from the yeast Arxula adeninivorans. Yeast 26 (2009) 83-93. https://dx.doi.org/10.1002/yea.1653

Böer E, Steinborn G, Florschütz K, Körner M, Gellissen G, Kunze G:

Arxula adeninivorans (Blastobotrys adeninivorans) – a dimorphic yeast of great biotechnological potential. In: Satyanarayana T, Kunze G (Eds.): Yeast biotechnology: diversity and applications. Dordrecht: Springer (2009) ISBN 978-1-402-08291-7, 615-634. https://dx.doi.org/10.1007/978-1-4020-8292-4_27

Breuer F-S:

Arxula adeninivorans als Produzent rekombinanter Tannase. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2009) 85 pp.

Knaust J:

Entwicklung eines auf Arxula adeninivorans basierenden Assays zur Erfassung androgen wirkender Substanzen in Umweltproben. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2009) 104 pp.

Körner M, Uhlig S, Heide K, Simon K, Kaiser C, Kunath K, Florschütz K, Suckau K, Fischer E, Kunze G:

Detection of estrogenic activities in environmental samples, urine and milk by a novel yeast biosensor. In: Kozlowski R, Zaikov G, Pudel F (Eds.): Renewable resources - obtaining, processing and applying. New York: Nova Science Publishers (2009) ISBN 978-1-606-92534-8, 79-86.

Körner M, Uhlig S, Heide K, Simon K, Kaiser C, Kunath K, Gerlach T, Florschütz K, Kunze G:

Nachweis von endokrinen Substanzen in Abwasser und Klärschlamm mit Hilfe eines auf Hefezellen basierenden Biosensors. In: Bilitewski B, Werner P, Gehring M J (Eds.): 4. Dresdner Symposium Endokrin aktive Stoffe in Abwasser, Klärschlamm und Abfällen: Tagungsband zum Symposium am 25. März 2009 in Dresden. (Series: Beiträge zu Abfallwirtschaft, Altlasten, Vol. 61) Pirna: Forum für Abfallwirtschaft und Altlasten e.V. (2009) ISBN 978-3-934253-53-7, 17-26.

Kunze G, Hang A H, Gellissen G:

Hansenula polymorpha (Pichia angusta): biology and application. In: Satyanarayana T, Kunze G (Eds.): Yeast biotechnology: diversity and applications. Dordrecht: Springer (2009) ISBN 978-1-402-08291-7, 47-64. https://dx.doi.org/10.1007/978-1-4020-8292-4_3

Satyanarayana T, Kunze G (Eds.):

Yeast biotechnology: diversity and applications. [Dordrecht]: Springer (2009) ISBN 978-1-402-08291-7, 744 pp.

Scholz A:

Expression bakterieller Gene zur PHA-Synthese in Arxula adeninivorans und Untersuchungen zum Stoffwechsel der Hefe. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität, Mathematisch-Naturwissenschaftliche Fakultät (2009) 140 pp.

Steinborn G, Kunze G, Gellissen G:

A wide range integrative expression vector (CoMed) system for yeasts. In: Satyanarayana T, Kunze G (Eds.): Yeast biotechnology: diversity and applications. Dordrecht: Springer (2009) ISBN 978-1-402-08291-7, 357-368. https://dx.doi.org/10.1007/978-1-4020-8292-4_17

Trautwein A:

Der Einfluss der Mannitol-Dehydrogenase auf die intrazelluläre Mannitiol-Akkumulation in der Hefe Arxula adeninivorans. (Diploma Thesis) Greifswald, Ernst-Moritz-Arndt-Universität (2009) 97 pp.

Watzke K:

Differenzielle Expression von Glomus intraradices Genen in unterschiedlichen Entwicklungsstadien der arbuskulären Mykorrhiza. (PhD Thesis) Greifswald, Ernst-Moritz-Arndt-Universität, Mathematisch-Naturwissenschaftliche Fakultät (2009) 138 pp.

2008

Gerlach T:

Optimierung eine Blastobotrys–Hefezellen basierenden Assays zur Erfassung östrogener Wirkungen im Rahmen der Umwelt-, Nahrungs- und Futtermittelanalytik. (Diploma Thesis) Greifswald Universität Greifswald (2008) 99 pp.

Haack M:

Entwicklung einer Fluoreszenzmessmethode zum Nachweis von Polyhydroxyalkanoaten in transgenen Arxula adeninivorans Stämmen. (Diploma Thesis) Köthen, Hochschule Anhalt (FH) (2008) 107 pp.

Kaiser C, Körner M, Uhlig S, Simon K, Kunath K, Suckau K, Kunze G:

Hormon-Biosensorsystem – schneller und eindeutiger Nachweis von Hormonen in Urin, Blutserum und Milch bei Haustieren. 14. Mitteldeutscher Schweine-Workshop in Bernburg – Wissenschaftliche Beiträge der Hochschule Anhalt (2008) 82-88.

Kunze G, Körner M, Uhlig S, Simon K:

Mit biologischem Testsystem gegen die Wasserverschmutzung. Hefezellenassay zur Erfassung von Östrogenen und östrogen wirksamen Substanzen. GIT Labor-Fachzeitschr. 4 (2008) 374-375.

Kunze G, Körner M, Uhlig S, Simon K:

Sibirischer Überlebenskünstler. GENIAL – Biotechnologie im Alltag (2008) 14-15.

Melmer G, Kunze G, Gellissen G:

Recombinant vaccine production in yeast. Biopharm Int. 1 (2008) 8-13.

Schröter A:

Nitratregulierende Promotoren als neue Elemente der Arxula adeninivorans Plattform. (Diploma Thesis) Köthen, Hochschule Anhalt (FH) (2008) 81 pp.

2007

Böer E, Steinborn G, Kunze G, Gellissen G:

Yeast expression platforms. Appl. Microbiol. Biotechnol. 77 (2007) 513-523. https://dx.doi.org/10.1007/s00253-007-1209-0

Böer E, Steinborn G, Matros A, Mock H-P, Gellissen G, Kunze G:

Production of interleukin-6 in Arxula adeninivorans, Hansenula polymorpha and Saccharomyces cerevisiae by applying the wide-range yeast vector (CoMedTM) system to simultaneous comparative assessment. FEMS Yeast Res. 7 (2007) 1181-1187. https://dx.doi.org/10.1111/j.1567-1364.2007.00254.x

El Fiki A, Metabteb G E, Bellebna C, Wartmann T, Bode R, Gellissen G, Kunze G:

The Arxula adeninivorans ATAL gene encoding transaldolase-gene characterization and biotechnological exploitation. Appl. Microbiol. Biotechnol. 74 (2007) 1292-1299. https://dx.doi.org/10.1007/s00253-006-0785-8

Heide K:

Etablierung und Validierung eines online-Analysators zur Erfassung östrogen-wirksamer Substanzen mittels Hefezellen-Dickschichtsensors. (Diploma Thesis) Köthen, Hochschule Anhalt (FH) (2007) 76 pp.

Kaur P, Kunze G, Satyanarayana T:

Yeast phytases: present scenario and future perspectives. Crit. Rev. Biotechnol. 27 (2007) 93-109. https://dx.doi.org/10.1080/07388550701334519

Kaur P, Lingner A, Singh B, Böer E, Polajeva J, Steinborn G, Bode R, Gellissen G, Satyanarayana T, Kunze G:

APHO1 from the yeast Arxula adeninivorans encodes an acid phosphatase of broad substrate specificity. Anton. Leeuw. Int. J. G. 91 (2007) 45-55. https://dx.doi.org/10.1007/s10482-006-9094-6

Minocha N, Kaur P, Satyanarayana T, Kunze G:

Acid phosphatase production by recombinant Arxula adeninivorans. Appl. Microbiol. Biotechnol. 76 (2007) 387-393. https://dx.doi.org/10.1007/s00253-007-1021-x

Müller I:

Arxula adeninivorans als Produzent von Polyhydroxyalkanoaten (PHA) – Expression der Gene phbA und phbB von Ralstonia euthropha. (Diploma Thesis) Köthen, Hochschule Anhalt (FH) (2007) 72 pp.

Steinborn G, Gellissen G, Kunze G:

A novel vector element providing multicopy vector integration in Arxula adeninivorans. FEMS Yeast Res. 7 (2007) 1197-1205. https://dx.doi.org/10.1111/j.1567-1364.2007.00280.x

Steinborn G, Wartmann T, Gellissen G, Kunze G:

Construction of an Arxula adeninivorans host-vector system based on trp1 complementation. J. Biotechnol. 127 (2007) 392-401. https://dx.doi.org/10.1016/j.jbiotec.2006.07.026

Straube N:

Synthese und Analyse von rekombinanter Anthocyanase aus Schizosaccharomyces pombe in Arxula adeninivorans. (Diploma Thesis) Köthen, Hochschule Anhalt (FH) (2007) 70 pp.

Tag K, Riedel K, Bauer H J, Hanke G, Baronian K H R, Kunze G:

Amperometric detection of Cu2+ by yeast biosensors using Flow Injection Analysis (FIA). Sensors Actuators B 122 (2007) 403-409. https://dx.doi.org/10.1016/j.snb.2006.06.007

Tulke D:

Hefezellen als Produzenten von rekombinanten Anthocyanasen. (Bachelor Thesis) Köthen, Hochschule Anhalt (FH) (2007) 91 pp.

2006

Hahn T, Tag K, Riedel K, Uhlig S, Baronian K, Gellissen G, Kunze G:

A novel estrogen sensor based on recombinant Arxula adeninivorans cells. Biosens. Bioelectron. 21 (2006) 2078-2085. https://dx.doi.org/10.1016/j.bios.2005.10.019

Krüger Y:

Nachweis von Glomus intraradices aus DNA-Proben mittels eines Fluoreszenz-Hybridisierungs-Assays. (Diploma Thesis) Braunschweig, Technische Universität Braunschweig (2006) 67 pp.

Kunze G, Riedel K:

Microbial biosensors for toxicity testing. In: Riedel K, Kunze G, Baronian K H R (Eds.): Biosensors and bioassays based on microorganisms. Trivandrum, Kerala, India: Research Signpost (2006) ISBN 81-3080038-1, 105-129.

Müller I:

Konstruktion von PHA produzierenden Arxula adeninivorans Stämmen zur Expression der Ralstonia eutropha Gene phbA und phbB. (Diploma Thesis) Köthen, Hochschule Anhalt (FH) (2006) 86 pp.

Riedel K, Kunze G:

Yeasts as microbial components for determination of Biochemical Oxygen Demand (BOD). In: Riedel K, Kunze G, Baronian K H R (Eds.): Biosensors and bioassays based on microorganisms. Trivandrum, Kerala, India: Research Signpost (2006) ISBN 81-3080038-1, 73-86.

Riedel K, Kunze G:

Fundamentals of microbial biosensors. In: Riedel K, Kunze G, Baronian K H R (Eds.): Biosensors and bioassays based on microorganisms. Trivandrum, Kerala, India: Research Signpost (2006) ISBN 81-3080038-1, 1-19.

Riedel K, Kunze G, Baronian K H R (Eds.):

Biosensors and bioassays based on microorganisms. Trivandrum, Kerala, India: Research Signpost (2006) ISBN 81-3080038-1, 253 pp.

Steinborn G, Boer E, Scholz A, Tag K, Kunze G, Gellissen G:

Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts. Microb. Cell Fact. 5 (2006) 33. https://dx.doi.org/10.1186/1475-2859-5-33

Tag K, Hahn T, Riedel K, Kunze G:

Assays and microbial biosensors for detection of endocrine disruptors (EDs). In: Riedel K, Kunze G, Baronian K H R (Eds.): Biosensors and bioassays based on microorganisms. Trivandrum, Kerala, India: Research Signpost (2006) ISBN 81-3080038-1, 211-228.

Tag K, Riedel K, Baronian K H R, Kunze G:

Determination of heavy metal ions by microbial biosensors. In: Riedel K, Kunze G, Baronian K H R (Eds.): Biosensors and bioassays based on microorganisms. Trivandrum, Kerala, India: Research Signpost (2006) ISBN 81-3080038-1, 193-209.