© Leibniz-Institut (IPK)


The independent research group Meiosis (ME) primarily funded by the German Federal Ministry of Education and Research (project HERBY) and the European Research Council (project MEIOBARMIX) is interested in the process of plant meiosis that generates genetic variation through homologous recombination.

Our research is closely-linked to the research theme of the department Breeding Research and we contribute with our research to the IPK research theme FSP3 "Mechanisms of Reproduction".

Barley (Hordeum vulgare) is an important cereal crop grown in a variety of climates globally used as animal fodder, food and for brewing. Traditional plant breeding, that harnesses natural genetic variation arising during meiosis, will have a key role to deliver crop improvements in the near future.

Meiosis assures genetic variation by regulated parental genetic exchange based on homologous recombination. However, particularly in cereal crops including barley meiotic recombination events are limited and skewed towards chromosome ends whereby a large portion of the genetic information keeps untapped in breeding programs.

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Embarking on molecular genetics, biochemistry and cytogenetic approaches, the overarching aim of the group is to alter the outcome of meiotic recombination with a focus on barley. To do so, projects in the group aim to:

  • better understand the mechanisms underlying the control of homologous recombination in both barley and Arabidopsis thaliana, 
  • to develop novel tools and strategies to study and alter meiotic recombination outcome,
  • and to translate acquired knowledge into barley.

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Amutha Suriya amutha@ipk-gatersleben.de+49 39482 5-683
ORCID iD icon0000-0002-2940-4354
CuacosDr. Maria cuacos@ipk-gatersleben.de+49 39482 5-216
ORCID iD icon0000-0003-4910-7311
Dabhi Riddhi Vijay dabhi@ipk-gatersleben.de
FengDr. Chao feng@ipk-gatersleben.de+49 39482 5-216
ORCID iD icon0000-0002-1422-0296
HeckmannDr. Stefan heckmann@ipk-gatersleben.de
ORCID iD icon0000-0002-0189-8428
KaridasDr. Premananda karidas@ipk-gatersleben.de+49 39482 5-673
Scientific guests / Fellowship
Wang Baicui wangb@ipk-gatersleben.de+49 39482 5-673
Lab Technicians
Doelling Marius doelling@ipk-gatersleben.de+49 39482 5-403
Hartmann Franziska hartmannf@ipk-gatersleben.de+49 39482 5-140
Lorenz Jana lorenz@ipk-gatersleben.de+49 39482 5-403

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Ahn Y J, Fuchs J, Houben A, Heckmann S:

High throughput measuring of meiotic recombination rates in barley pollen nuclei using Crystal Digital PCR TM. Plant J. 107 (2021) 649-661. https://dx.doi.org/10.1111/tpj.15305

Cuacos M, Heckmann S:

Arabidopsis thaliana. Brassicaceae (Calender Page). In: Houben A (Ed.): Chromosome Biology 2021. 4th Edition. Gatersleben, Germany: IPK (2021) 1.

Cuacos M, Lambing C, Pachon-Penalba M, Osman K, Armstrong S J, Henderson I R, Sanchez-Moran E, Franklin F C H, Heckmann S:

Meiotic chromosome axis remodelling is critical for meiotic recombination in Brassica rapa. J. Exp. Bot. 72 (2021) 3012-3027. https://dx.doi.org/10.1093/jxb/erab035


Ahn Y-J, Cuacos M, Ayoub M A, Kappermann J, Houben A, Heckmann S:

In planta delivery of chemical compounds into barley meiocytes – EdU as compound example. In: Pradillo M, Heckmann S (Eds.): Plant Meiosis: methods and protocols. (Series: Methods in molecular biology, Vol. 2061) New York, NY: Humana Press (2020) 381-402. dx.doi.org/10.1007/978-1-4939-9818-0_27 978-1-4939-9817-3

Chen E C H, Mathieu S, Hoffrichter A, Ropars J, Dreissig S, Fuchs J, Brachmann A, Corradi N:

More filtering on SNP calling does not remove evidence of inter-nucleus recombination in dikaryotic arbuscular mycorrhizal fungi. Front. Plant Sci. 11 (2020) 912. https://dx.doi.org/10.3389/fpls.2020.00912

Desjardins S D, Ogle D E, Ayoub M A, Heckmann S, Henderson I R, Edwards K J, Higgins J D:

MutS homologue 4 and MutS homologue 5 maintain the obligate crossover in wheat despite stepwise gene loss following polyploidization. Plant Physiol. 183 (2020) 1545-1558. https://dx.doi.org/10.1104/pp.20.00534

Dreissig S, Fuchs J, Himmelbach A, Mascher M, Houben A:

Quantification of recombination rate and segregation distortion by genotyping and sequencing of single pollen nuclei. In: Pradillo M, Heckmann S (Eds.): Plant Meiosis: methods and protocols. (Series: Methods in molecular biology, Vol. 2061) New York, NY: Humana Press (2020) 281-300. dx.doi.org/10.1007/978-1-4939-9818-0_20 978-1-4939-9817-3

Dubey R:

Targeted meiotic DNA Double strand break induction using the CRISPR/Cas9 system analyzed via single pollen genotyping. (Master Thesis) Zaragoza, Catalonia, Spain, Spain University of Lleida, Instituto Agronómico Mediterráneo de Zaragoza (IAMZ-CIHEAM) (2020) 70 pp.

Garavello M, Cuenca J, Dreissig S, Fuchs J, Navarro L, Houben A, Aleza P:

Analysis of crossover events and allele segregation distortion in interspecific citrus hybrids by single pollen genotyping. Front. Plant Sci. 11 (2020) 615. https://dx.doi.org/10.3389/fpls.2020.00615

Khosravi S, Dreissig S, Schindele P, Wolter F, Rutten T, Puchta H, Houben A:

Live Cell CRISPR imaging in plant cells with a telomere-specific guide RNA. In: Heinlein M (Ed.): RNA Tagging: methods and protocols. (Series: Methods in molecular biology, Vol. 2166) New York, NY: Humana Press (2020) 343-356. dx.doi.org/10.1007/978-1-0716-0712-1_20 1940-6029 (Electronic)1064-3745 (Linking)

Khosravi S, Ishii T, Dreissig S, Houben A:

Application and prospects of CRISPR/Cas9-based methods to trace defined genomic sequences in living and fixed plant cells. Chromosome Res. 28 (2020) 7-17. https://dx.doi.org/10.1007/s10577-019-09622-0

Le Goff S, Keçeli B N, Jeřábková H, Heckmann S, Rutten T, Cotterell S, Schubert V, Roitinger E, Mechtler K, Franklin F C H, Tatout C, Houben A, Geelen D, Probst A V, Lermontova I:

The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis. Plant J. 101 (2020) 71-86. https://dx.doi.org/10.1111/tpj.14518

Pradillo M, Heckmann S (Eds.):

Plant Meiosis: methods and protocols. (Series: Methods in molecular biology, Vol. 2061) New York, NY: Humana Press (2020) 410 pp. dx.doi.org/10.1007/978-1-4939-9818-0 978-1-4939-9817-3

Schmidt C, Fransz P, Rönspies M, Dreissig S, Fuchs J, Heckmann S, Houben A, Puchta H:

Changing local recombination patterns in plants by CRISPR/Cas mediated chromosome engineering. Nat. Commun. 11 (2020) 4418. https://dx.doi.org/10.1038/s41467-020-18277-z

Schubert V, Neumann P, Marques A, Heckmann S, Macas J, Pedrosa-Harand A, Schubert I, Jang T-S, Houben A:

Super-resolution microscopy reveals diversity of plant centromere architecture. Int. J. Mol. Sci. 21 (2020) 3488. https://dx.doi.org/10.3390/ijms21103488

Valuchova S, Mikulkova P, Pecinkova J, Klimova J, Krumnikl M, Bainar P, Heckmann S, Tomancak P, Riha K:

Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy. eLife 9 (2020) e52546. https://dx.doi.org/10.7554/eLife.52546


Demidov D, Heckmann S, Weiss O, Rutten T, Tomaštíková E D, Kuhlmann M, Scholl P, Municio C M, Lermontova I, Houben A:

Deregulated phosphorylation of CENH3 at Ser65 affects the development of floral meristems in Arabidopsis thaliana. Front. Plant Sci. 10 (2019) 928. https://dx.doi.org/10.3389/Fpls.2019.00928

Dreissig S, Mascher M, Heckmann S:

Variation in recombination rate is shaped by domestication and environmental conditions in barley. Mol. Biol. Evol. 36 (2019) 2029–2039. https://dx.doi.org/10.1093/molbev/msz141

Ishii T, Schubert V, Khosravi S, Dreissig S, Metje-Sprink J, Sprink T, Fuchs J, Meister A, Houben A:

RNA-guided endonuclease – in situ labelling (RGEN-ISL): a fast CRISPR/Cas9-based method to label genomic sequences in various species. New Phytol. 222 (2019) 1652-1661. https://dx.doi.org/10.1111/nph.15720


Chambon A, West A, Vezon D, Horlow C, De Muyt A, Chelysheva L, Ronceret A, Darbyshire A R, Osman K, Heckmann S, Franklin F C H, Grelon M:

Identification of ASYNAPTIC4, a component of the meiotic chromosome axis. Plant Physiol. 178 (2018) 233–246. https://dx.doi.org/10.1104/pp.17.01725

Chen E C, Mathieu S, Hoffrichter A, Sedzielewska-Toro K, Peart M, Pelin A, Ndikumana S, Ropars J, Dreissig S, Fuchs J, Brachmann A, Corradi N:

Single nucleus sequencing reveals evidence of inter-nucleus recombination in arbuscular mycorrhizal fungi. eLife 7 (2018) e39813. https://dx.doi.org/10.7554/eLife.39813

Lambing C, Heckmann S:

Tackling plant meiosis: from model research to crop improvement. Front. Plant Sci. 9 (2018) 829. https://dx.doi.org/10.3389/fpls.2018.00829

Osman K, Yang J, Roitinger E, Lambing C, Heckmann S, Howell E, Cuacos M, Imre R, Dürnberger G, Mechtler K, Armstrong S, Franklin F C H:

Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein ASY1 and a network of associated proteins at prophase I of meiosis. Plant J. 93 (2018) 17-33. https://dx.doi.org/10.1111/tpj.13752


Kappermann J:

Beeinflussung der Anzahl und Verteilung meiotischer Rekombinationsereignisse in der Gerste (Hordeum vulgare) mittels Manipulation epigenetischer Faktoren anhand von Chemikalien. (Bachelor Thesis) Köthen, Hochschule Anhalt (2017)

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