Chen J, Liu T, Xia Y, Barth L, Plieske J, Gnad H, Tamilselvan-Nattar-Amutha S, Qi Z, Heckmann S, Houben A:

Satellite DNA-targeted CRISPR/Cas9-mediated editing enables chromosome truncation and elimination in wheat. Plant Commun. (2026) in press. https://dx.doi.org/10.1016/j.xplc.2026.101833

Feng C, Wang B, Cuacos M, Lorenz J, Hartmann F, Heckmann S:

FeM-ID: A biotin labeling-based approach for the dissection of female meiotic chromosome behavior in Arabidopsis thaliana. Plant Cell 38 (2026) koag024. https://dx.doi.org/10.1093/plcell/koag024

Piskorz E W, Steckenborn S, Cuacos M, Heckmann S:

Meiosis-specific protein co-expression in Arabidopsis thaliana: a protein delivery tool in meiotic cells. Plant J. 125 (2026) e70811. https://dx.doi.org/10.1111/tpj.70811

Somasundaram S, Yaşar S, Fuchs J, Cuacos M, Claassen J, Weiss O, Kochevenko A, Lamb J C, Li T, Capdeville N, Puchta H, Houben A:

Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction. Plant Commun. (2026) in press. https://dx.doi.org/10.1016/j.xplc.2026.101837

Tamilselvan-Nattar-Amutha S, Fuchs J, Heckmann S:

Crystal Digital PCR-based single nucleus pollen genotyping in barley (Hordeum vulgare L.) to measure meiotic crossover rates in defined chromosomal intervals. In: Pavlovic G (Ed.): Digital PCR. Methods and protocols. (Series: Methods in molecular biology, Vol. 2969) New York: Humana New York (2026) 978-1-0716-4766-0, 87-100. https://dx.doi.org/10.1007/978-1-0716-4767-7_6

Cuacos M, Heckmann S:

Diversity favoured: heterozygosity attracts crossovers. Nat. Plants 11 (2025) 1706-1707. https://dx.doi.org/10.1038/s41477-025-02066-x

Feng C, Lorenz J, Dreissig S, Schubert V, Wang B, Hartmann F, Cuacos M, Fernández-Jiménez N, Zhao Z, Eggeling C, Câmara A S, Himmelbach A, Heckmann S:

The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana. Nat. Plants 11 (2025) 1353-1366. https://dx.doi.org/10.1038/s41477-025-02030-9

Feng J-W, Pidon H, Cuacos M, Lux T, Himmelbach A, Haghi R, Fuchs J, Haberer G, Kuo Y-T, Guo Y, Jayakodi M, Toegelová H, Harpke D, Knauft M, Fiebig A, Maruschewski M, Ronen M, Sharon A, Šimková H, Mayer K F X, Spannagl M, Kumlehn J, Heckmann S, Houben A, Blattner F R, Stein N, Mascher M:

A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum. Nature 645 (2025) 429-438. https://dx.doi.org/10.1038/s41586-025-09270-x

Schüler D, Lange M, Altmann T, Cuacos M, Arend D, D’Auria J C, Fiebig A, Kumlehn J, Neumann K, Melzer M, Rey-Mazón E, Rolletschek H, Scholz U, Willner E, Reif J C:

Data management in balance – a decade of balancing pragmatism, sustainability and innovation at plant research center IPK Gatersleben. J. Integr. Bioinform. 22 (2025) 20250012. https://dx.doi.org/10.1515/jib-2025-0012

Crhak Khaitova L, Mikulkova P, Pecinkova J, Kalidass M, Heckmann S, Lermontova I, Riha K:

Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis. eLife 12 (2024) e90253. https://dx.doi.org/10.7554/eLife.90253

Ahmadli U, Kalidass M, Crhak Khaitova L, Fuchs J, Cuacos M, Demidov D, Zuo S, Pecinkova J, Mascher M, Ingouff M, Heckmann S, Houben A, Riha K, Lermontova I:

High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis. Plant Commun. 4 (2023) 100507. https://dx.doi.org/10.1016/j.xplc.2022.100507

Ayoub M A:

Exploration of strategies to alter the meiotic recombination landscape in barley (Hordeum vulgare). (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät III Agrar- und Ernährungswissenschaften, Geowissenschaften und Informatik (2023) 107 pp.

Feng C, Roitinger E, Hudecz O, Cuacos M, Lorenz J, Schubert V, Wang B, Wang R, Mechtler K, Heckmann S:

TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana. Nat. Plants 9 (2023) 616-630. https://dx.doi.org/10.1038/s41477-023-01371-7

Piskorz E W, Xu L, Ma Y, Jiang H:

Double-haploid induction generates extensive differential DNA methylation in Arabidopsis. J. Exp. Bot. 74 (2023) 835-847. https://dx.doi.org/10.1093/jxb/erac397

Steckenborn Coria S:

Towards the induction of site-directed meiotic recombination. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät III Agrar- und Ernährungswissenschaften, Geowissenschaften und Informatik (2023) 106 pp.

Steckenborn S, Cuacos M, Ayoub M A, Feng C, Schubert V, Hoffie I, Hensel G, Kumlehn J, Heckmann S:

The meiotic topoisomerase VI B subunit (MTOPVIB) is essential for meiotic DNA double-strand break formation in barley (Hordeum vulgare L.). Plant Reprod. 36 (2023) 1-15. https://dx.doi.org/10.1007/s00497-022-00444-5

Tamilselvan-Nattar-Amutha S, Hiekel S, Hartmann F, Lorenz J, Dabhi R V, Dreissig S, Hensel G, Kumlehn J, Heckmann S:

Barley stripe mosaic virus-mediated somatic and heritable gene editing in barley (Hordeum vulgare L.). Front. Plant Sci. 14 (2023) 1201446. https://dx.doi.org/10.3389/fpls.2023.1201446

Ahn Y-J:

Development and application of Crystal Digital PCR-based single pollen nucleus genotyping to measure meiotic recombination rates in barley (Hordeum vulgare) in high-throughput. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Institut für Agrar- und Ernährungswissenschaften der Naturwissenschaftlichen Fakultät III (2022) 118 pp.

Randall R S, Jourdain C, Nowicka A, Kaduchová K, Kubová M, Ayoub M A, Schubert V, Tatout C, Colas I, Kalyanikrishna, Desset S, Mermet S, Boulaflous-Stevens A, Kubalová I, Mandáková T, Heckmann S, Lysak M A, Panatta M, Santoro R, Schubert D, Pecinka A, Routh D, Baroux C:

Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes. Nucleus 13 (2022) 277-299. https://dx.doi.org/10.1080/19491034.2022.2144013

Schreiber M, Gao Y, Koch N, Fuchs J, Heckmann S, Himmelbach A, Börner A, Özkan H, Maurer A, Stein N, Mascher M, Dreissig S:

Recombination landscape divergence between populations is marked by larger low-recombining regions in domesticated rye. Mol. Biol. Evol. 39 (2022) msac131. https://dx.doi.org/10.1093/molbev/msac131

Ahn Y-J, Fuchs J, Houben A, Heckmann S:

High throughput measuring of meiotic recombination rates in barley pollen nuclei using Crystal Digital PCR TM. Plant J. 107 (2021) 649-661. https://dx.doi.org/10.1111/tpj.15305

Cuacos M, Heckmann S:

Arabidopsis thaliana. Brassicaceae (Calender Page). In: Houben A (Ed.): Chromosome Biology 2021. 4th Edition. Gatersleben, Germany: IPK (2021) 1.

Cuacos M, Lambing C, Pachon-Penalba M, Osman K, Armstrong S J, Henderson I R, Sanchez-Moran E, Franklin F C H, Heckmann S:

Meiotic chromosome axis remodelling is critical for meiotic recombination in Brassica rapa. J. Exp. Bot. 72 (2021) 3012-3027. https://dx.doi.org/10.1093/jxb/erab035

Ahn Y-J, Cuacos M, Ayoub M A, Kappermann J, Houben A, Heckmann S:

In planta delivery of chemical compounds into barley meiocytes – EdU as compound example. In: Pradillo M, Heckmann S (Eds.): Plant Meiosis: methods and protocols. (Series: Methods in molecular biology, Vol. 2061) New York, NY: Humana Press (2020) 978-1-4939-9817-3, 381-402. https://dx.doi.org/10.1007/978-1-4939-9818-0_27

Ayoub M A, Heckmann S:

Hordeum vulgare. Poaceae (Calender Page). In: Houben A (Ed.): Plant Cytogenetics 2020. 3th Edition. Gatersleben, Germany: IPK (2020) 1.

Chen E C H, Mathieu S, Hoffrichter A, Ropars J, Dreissig S, Fuchs J, Brachmann A, Corradi N:

More filtering on SNP calling does not remove evidence of inter-nucleus recombination in dikaryotic arbuscular mycorrhizal fungi. Front. Plant Sci. 11 (2020) 912. https://dx.doi.org/10.3389/fpls.2020.00912

Desjardins S D, Ogle D E, Ayoub M A, Heckmann S, Henderson I R, Edwards K J, Higgins J D:

MutS homologue 4 and MutS homologue 5 maintain the obligate crossover in wheat despite stepwise gene loss following polyploidization. Plant Physiol. 183 (2020) 1545-1558. https://dx.doi.org/10.1104/pp.20.00534

Dreissig S, Fuchs J, Himmelbach A, Mascher M, Houben A:

Quantification of recombination rate and segregation distortion by genotyping and sequencing of single pollen nuclei. In: Pradillo M, Heckmann S (Eds.): Plant Meiosis: methods and protocols. (Series: Methods in molecular biology, Vol. 2061) New York, NY: Humana Press (2020) 978-1-4939-9817-3, 281-300. https://dx.doi.org/10.1007/978-1-4939-9818-0_20

Dubey R:

Targeted meiotic DNA Double strand break induction using the CRISPR/Cas9 system analyzed via single pollen genotyping. (Master Thesis) Zaragoza, Catalonia, Spain, Spain University of Lleida, Instituto Agronómico Mediterráneo de Zaragoza (IAMZ-CIHEAM) (2020) 70 pp.

Garavello M, Cuenca J, Dreissig S, Fuchs J, Navarro L, Houben A, Aleza P:

Analysis of crossover events and allele segregation distortion in interspecific citrus hybrids by single pollen genotyping. Front. Plant Sci. 11 (2020) 615. https://dx.doi.org/10.3389/fpls.2020.00615

Khosravi S, Dreissig S, Schindele P, Wolter F, Rutten T, Puchta H, Houben A:

Live Cell CRISPR imaging in plant cells with a telomere-specific guide RNA. In: Heinlein M (Ed.): RNA Tagging: methods and protocols. (Series: Methods in molecular biology, Vol. 2166) New York, NY: Humana Press (2020) 1940-6029 (Electronic)1064-3745 (Linking), 343-356. https://dx.doi.org/10.1007/978-1-0716-0712-1_20

Khosravi S, Ishii T, Dreissig S, Houben A:

Application and prospects of CRISPR/Cas9-based methods to trace defined genomic sequences in living and fixed plant cells. Chromosome Res. 28 (2020) 7-17. https://dx.doi.org/10.1007/s10577-019-09622-0

Le Goff S, Keçeli B N, Jeřábková H, Heckmann S, Rutten T, Cotterell S, Schubert V, Roitinger E, Mechtler K, Franklin F C H, Tatout C, Houben A, Geelen D, Probst A V, Lermontova I:

The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis. Plant J. 101 (2020) 71-86. https://dx.doi.org/10.1111/tpj.14518

Pradillo M, Heckmann S (Eds.):

Plant Meiosis: methods and protocols. (Series: Methods in molecular biology, Vol. 2061) New York, NY: Humana Press (2020) 978-1-4939-9817-3, 410 pp. https://dx.doi.org/10.1007/978-1-4939-9818-0

Schmidt C, Fransz P, Rönspies M, Dreissig S, Fuchs J, Heckmann S, Houben A, Puchta H:

Changing local recombination patterns in plants by CRISPR/Cas mediated chromosome engineering. Nat. Commun. 11 (2020) 4418. https://dx.doi.org/10.1038/s41467-020-18277-z

Schubert V, Neumann P, Marques A, Heckmann S, Macas J, Pedrosa-Harand A, Schubert I, Jang T-S, Houben A:

Super-resolution microscopy reveals diversity of plant centromere architecture. Int. J. Mol. Sci. 21 (2020) 3488. https://dx.doi.org/10.3390/ijms21103488

Valuchova S, Mikulkova P, Pecinkova J, Klimova J, Krumnikl M, Bainar P, Heckmann S, Tomancak P, Riha K:

Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy. eLife 9 (2020) e52546. https://dx.doi.org/10.7554/eLife.52546

Demidov D, Heckmann S, Weiss O, Rutten T, Tomaštíková E D, Kuhlmann M, Scholl P, Municio C M, Lermontova I, Houben A:

Deregulated phosphorylation of CENH3 at Ser65 affects the development of floral meristems in Arabidopsis thaliana. Front. Plant Sci. 10 (2019) 928. https://dx.doi.org/10.3389/Fpls.2019.00928

Dreissig S, Mascher M, Heckmann S:

Variation in recombination rate is shaped by domestication and environmental conditions in barley. Mol. Biol. Evol. 36 (2019) 2029–2039. https://dx.doi.org/10.1093/molbev/msz141

Ishii T, Schubert V, Khosravi S, Dreissig S, Metje-Sprink J, Sprink T, Fuchs J, Meister A, Houben A:

RNA-guided endonuclease – in situ labelling (RGEN-ISL): a fast CRISPR/Cas9-based method to label genomic sequences in various species. New Phytol. 222 (2019) 1652-1661. https://dx.doi.org/10.1111/nph.15720

Chen E C, Mathieu S, Hoffrichter A, Sedzielewska-Toro K, Peart M, Pelin A, Ndikumana S, Ropars J, Dreissig S, Fuchs J, Brachmann A, Corradi N:

Single nucleus sequencing reveals evidence of inter-nucleus recombination in arbuscular mycorrhizal fungi. eLife 7 (2018) e39813. https://dx.doi.org/10.7554/eLife.39813

Lambing C, Heckmann S:

Tackling plant meiosis: from model research to crop improvement. Front. Plant Sci. 9 (2018) 829. https://dx.doi.org/10.3389/fpls.2018.00829

Kappermann J:

Beeinflussung der Anzahl und Verteilung meiotischer Rekombinationsereignisse in der Gerste (Hordeum vulgare) mittels Manipulation epigenetischer Faktoren anhand von Chemikalien. (Bachelor Thesis) Köthen, Hochschule Anhalt (2017)

Feng C, Lorenz J, Dreissig S, Schubert V, Wang B, Hartmann F, Cuacos M, Fernández-Jiménez N, Câmara A, Himmelbach A, Heckmann S:

The novel synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana. European Nucleotide Archive (ENA) at EMBL-EBI under accession number PRJEB81799 (2024) https://www.ebi.ac.uk/ena/data/view/PRJEB81799

Heckmann S:

LC-MSMS of Arabidopsis thaliana flower bud samples (TurboID-based proximity labeling of meiotic chromosome axes). ProteomeXchange Consortium via the PRIDE partner repository under accession number PXD034241 (2023) https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD034241