Abiotische Stress-Genomik

Forschungsfelder

Leitung: Dr. Markus Kuhlmann

Die Junior Arbeitsgruppe wird vom Ministerium für Bildung und Forschung im Rahmen des Interdisziplinären Zentrums für Nutzpflanzenforschung (IZN) gefördert. Es besteht eine starke Anbindung and die Martin-Luther-University Halle-Wittenberg.

Trockenheit während der letzen Lebensphase von Nutzpflanzen kann schwerwiegende Auswirkungen auf die Pflanzenproduktion haben. Besonders sensibel reagieren die Pflanzen wenn die Trockenheit in der reproduktiven Phase auftritt. In diesen Fällen kann es zu dramatischen Ertragsverlusten kommen. Unser Labor untersucht die molekularen Mechanismen, die an diesem Vorgang beteiligt sind. Unser Hauptaugenmerk liegt hierbei in der (a) Veränderung der Körnerzahl und der (b) Effizienz der Samenfüllung.  

Wir nutzen integrative genomische Ansätze, um Gen-regulatorische Netzwerke ableiten zu können. Desweiteren arbeiten wir daran wichtige Regulatoren, die zur Differenzierung des Ährenmeristems und zur Samenentwicklung beitragen, zu identifizieren. Hierfür nutzen wir gut definiertes genetisches Pflanzenmaterial:

  • Nicht-Gentechnisch veränderte Organismen (Non-GMO, hergestellt durch natürliche genetische Variation):  Untersucht werden Kartierungspopulationen von ausgesuchten Zuchtlinien, die einen phänotypischen Ergrünungs oder Remobilisierungscharakter unter Trockenheit zeigen.
  • GMO: Es werden Genetisch-veränderte Pflanzen verwendet um die Auswirkungen einer veränderten ABA Homöostase zu untersuchen. Hierfür werden Trockenstress induzierbare Promotoren verwandt, die auch in der generativen Phase der Pflanzen aktiv sind. 

Das oben genannte genetische Material wird verwendet, um die Mechanismen der Ährchenfruchtbarkeit zu analysieren. Der Mechanismus der die Ährchenfruchtbarkeit reguliert, beeinflußt neben der Kornzahl auch die Samenqualität. Hierbei spielt die Trockstress-Sensitivität während der Kornfüllungsphase eine wichtige Rolle.

Wir untersuchen weiterhin die Rolle von kleinen regulatorischen RNAs (miRNAs/ siRNAs) und der DNA Methylierung unter Trockenstress als vererbbarer Faktor der Trockenstressresistenz. Falls wir Ihr Interesse wecken konnten, können sie noch weitere Informationen unter RESEARCH PROJECTS finden.

Trockenheit während der letzen Lebensphase von Nutzpflanzen kann schwerwiegende Auswirkungen auf den Ertrag haben: Der Stärkegehalt (Blau) in den Gerstenkörnern ist unter Trockenstressbedingungen deutlich reduziert. Stresstolerante Pflanzen sind in der Lage unter gleichen Trockenstressbedingungen größere Körner zu entwickeln, was zu einem stabileren Ertrag führt. (Bild 1)

Durch Transkript- und Metabolit-Analysen von sich entwickelnden Samen unter unterschiedlichen Stressbedingungen und zu unterschiedlichen Zeitpunkten lassen sich Gen Netzwerke entwickeln, in denen wichtige Regulatoren identifiziert werden können. (Bild 2)

Forschungsstrategien

Wir verfolgen fünf unterschiedliche Strategien um die zentrale Frage nach der Ertragsstabilität und guten Samenqualität unter Trockenstressbedingungen zu beantworten:

(i) Erforschung noch nicht erschlossener genetischer Reserven von Nutzpflanzen um genetische Netzwerke zu identifizieren die zu agrarwirtschaftlich wichtigen Pflanzeneigenschaften beitragen. Hierfür werden -OMICS technologieen unter Kontroll und Trockenstressbedingungen eingesetzt.

(ii) Es wird die Rolle kleiner nicht codierende RNAs (siRNAs und miRNAs) und der DNA Methylierung von Genen während unter Trockenheit untersucht.

(iii) Das Wissen um die molekularen Mechanismen soll verwendet werden, um transgene Pflanzen mit verbesserten Eigenschaften unter schwierigen Umweltbedingungen zu erzeugen.

(iv) Um die Funktionalität des Konzeptes zu zeigen, wird eine Strategie verwandt um mittels eines transgen Ansatzes mehrere Gene zu modifizieren welches eine hormonale Homöostase im reproduktiven Gewebe ermöglichen soll.

(v) Es werden “top-down” Ansätze verfolgt, um Genregulatorische Netzwerke zu generieren, welche dazu beitragen die Ertragsstabilität unter Trockenheit und Hitze zu garantieren.

Publikationen

Autor
Titel
2024

Thirulogachandar V, Govind G, Hensel G, Kale S M, Kuhlmann M, Eschen-Lippold L, Rutten T, Koppolu R, Rajaraman J, Palakolanu S R, Seiler C, Sakuma S, Jayakodi M, Lee J, Kumlehn J, Komatsuda T, Schnurbusch T, Sreenivasulu N:

HOMEOBOX2, the paralog of SIX-ROWED SPIKE1/HOMEOBOX1, is dispensable for barley spikelet development. J. Exp. Bot. (2024) Epub ahead of print. https://dx.doi.org/10.1093/jxb/erae044

2019

Tedeschi F, Rizzo P, Huong B, Czihal A, Rutten T, Altschmied L, Scharfenberg S, Grosse I, Becker C, Weigel D, Bäumlein H, Kuhlmann M:

EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis. New Phytol. 221 (2019) 261-278. https://dx.doi.org/10.1111/nph.15439

2018

Döll S, Kuhlmann M, Rutten T, Mette M F, Scharfenberg S, Petridis A, Berreth D C, Mock H-P:

Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex. Plant J. 93 (2018) 431-444. https://dx.doi.org/10.1111/tpj.13797

Hashemipetroudi S H, Nematzadeh G, Ahmadian G, Yamchi A, Kuhlmann M:

Assessment of DNA contamination in RNA samples based on ribosomal DNA. J. Vis. Exp. 131 (2018) e55451. https://dx.doi.org/10.3791/55451

Wang H, Chen W, Eggert K, Charnikhova T, Bouwmeester H, Schweizer P, Hajirezaei M R, Seiler C, Sreenivasulu N, von Wirén N, Kuhlmann M:

Abscisic acid influences tillering by modulation of strigolactones in barley. J. Exp. Bot. 69 (2018) 3883-3898. https://dx.doi.org/10.1093/jxb/ery200

2017

de Guzman M K, Parween S, Butardo V M, Alhambra C M, Anacleto R, Seiler C, Bird A R, Chow C P, Sreenivasulu N:

Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination. Sci. Rep. 7 (2017) 5854. https://dx.doi.org/10.1038/s41598-017-06026-0

Radchuk V, Riewe D, Peukert M, Matros A, Strickert M, Radchuk R, Weier D, Steinbiß H-H, Sreenivasulu N, Weschke W, Weber H:

Down-regulated sucrose transporters HvSUT1, HvSUT2 affects sucrose homeostasis along its delivery path in barley grains. J. Exp. Bot. 68 (2017) 4595-4612. https://doi.org/10.1093/jxb/erx266

Sandmann M, Talbert P, Demidov D, Kuhlmann M, Rutten T, Conrad U, Lermontova I:

Targeting of A. thaliana KNL2 to centromeres depends on the conserved CENPC-k motif in its C-terminus. Plant Cell 29 (2017) 144-155. https://dx.doi.org/10.1105/tpc.16.00720

Surdonja K, Eggert K, Hajirezaei M-R, Harshavardhan V, Seiler C, von Wirén N, Sreenivasulu N, Kuhlmann M:

Increase of DNA methylation at the HvCKX2.1 promoter by terminal drought stress in barley. Epigenomes 1 (2017) 9. https://dx.doi.org/10.3390/epigenomes1020009

Thirulogachandar V, Alqudah A M, Koppolu R, Rutten T, Graner A, Hensel G, Kumlehn J, Bräutigam A, Sreenivasulu N, Schnurbusch T, Kuhlmann M:

Leaf primordium size specifies leaf width and vein number among row-type classes in barley. Plant J. 91 (2017) 601-612. https://dx.doi.org/10.1111/tpj.13590

Venkatasubbu T:

Dosage of duplicated and antifunctionalized homeobox proteins influences leaf and spikelet development in barley (Hordeum vulgare L.). (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I - Biowissenschaften (2017) 165 pp.

Wang H:

Genetic manipulation of the cross-talk between abscisic acid and strigolactones and their biosynthetic link during late tittelring in barley. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2017) 114 pp.

2016

Hashemi S H, Nematzadeh G, Ahmadian G, Yamchi A, Kuhlmann M:

Identification and validation of Aeluropus littoralis reference genes for quantitative real-time PCR normalization. J. Biol. Res. (Thessalon.) 23 (2016) 18. https://dx.doi.org/10.1186/s40709-016-0053-8

Hashemipetroudi S H, Nematzadeh G, Ahmadian G, Yamchi A, Kuhlmann M:

Expression analysis of salt stress related expressed sequence tags (ESTs) from Aeluropus littoralis by quantitative real-time PCR. Biosci. Biotech. Res. Comm. 9 (2016) 445-456.

Hosseini S A, Hajirezaei M-R, Seiler C, Sreenivasulu N, von Wiren N:

A potential role of flag leaf potassium in conferring tolerance to drought-Induced leaf senescence in barley. Front. Plant Sci. 7 (2016) 206. https://dx.doi.org/10.3389/fpls.2016.00206

Yamunarani R, Ramegowda V, Govind G, Jalendrakumar H G, Udayakumar M, Shankar A G:

Effect of Zn application on its uptake, distribution and concentration of Fe and Cu in finger millet [Eleusine coracana (L.) Gaertn.]. J. Plant Nutr. 39 (2016) 569-580. https://dx.doi.org/10.1080/01904167.2016.1143490

2015

Dixit S, Kumar Biswal A, Min A, Henry A, Oane R H, Raorane M L, Longkumer T, Pabuayon I M, Mutte S K, Vardarajan A R, Miro B, Govindan G, Albano-Enriquez B, Pueffeld M, Sreenivasulu N, Slamet-Loedin I, Sundarvelpandian K, Tsai Y C, Raghuvanshi S, Hsing Y I, Kumar A, Kohli A:

Action of multiple intra-QTL genes concerted around a co-localized transcription factor underpins a large effect QTL. Sci. Rep. 5 (2015) 15183. https://dx.doi.org/10.1038/srep15183

Jagadish K S V, Kavi Kishor P, Bahuguna R, von Wirén N, Sreenivasulu N:

Staying alive or going to die during terminal senescence – an enigma surrounding yield stability. Front. Plant Sci. 6 (2015) 1070. https://dx.doi.org/10.3389/fpls.2015.01070

Kavi Kishor P B, Hima Kumari P, Sunita M S, Sreenivasulu N:

Role of proline in cell wall synthesis and plant development and its implications in plant ontogeny. Front. Plant Sci. 6 (2015) 544. https://dx.doi.org/10.3389/fpls.2015.00544

Kuhlmann M (Ed.):

Barlomics –omic approaches in the barley field. Abstractbook of the 2nd IPK Barlomics Summer School 2015. (2015) 64 pp.

Marques A, Ribeiro T, Neumann P, Macas J, Novák P, Schubert V, Pellino M, Fuchs J, Ma W, Kuhlmann M, Brandt R, Vanzela A L L, Beseda T, Šimková H, Pedrosa-Harand A, Houben A:

Holocentromeres in Rhynchospora are associated with genome-wide centromere-specific repeat arrays interspersed amongst euchromatin. Proc. Natl. Acad. Sci. U.S.A. 112 (2015) 13633–13638. https://dx.doi.org/10.1073/pnas.1512255112

Meyer R C, Hönig G, Brandt R, Arana-Ceballos F, Neitsch C, Reuter G, Altmann T, Kuhlmann M:

Overexpression of Arabidopsis thaliana ERI, the homolog of C. elegans Enhancer of RNAinterference, leads to enhanced growth. Front. Plant Sci. 6 (2015) 531. https://dx.doi.org/10.3389/fpls.2015.00531

Poursarebani N, Seidensticker T, Koppolu R, Trautewig C, Gawroński P, Bini F, Govind G, Rutten T, Sakuma S, Tagiri A, Wolde G M, Youssef H M, Battal A, Ciannamea S, Fusca T, Nussbaumer T, Pozzi C, Börner A, Lundqvist U, Komatsuda T, Salvi S, Tuberosa R, Uauy C, Sreenivasulu N, Rossini L, Schnurbusch T:

The genetic basis of composite spike form in barley and Miracle-Wheat"." Genetics 201 (2015) 155-165. https://dx.doi.org/10.1534/genetics.115.176628

Raorane M L, Pabuayon I M, Miro B, Kalladan R, Hajirezaei M-R, Oane R H, Kumar A, Sreenivasulu N, Henry A, Kohli A:

Variation in primary metabolites in parental and near-isogenic lines of the QTL qDTY12.1: altered roots and flag leaves but similar spikelets of rice under drought. Mol. Breed. 35 (2015) 138. https://dx.doi.org/10.1007/s11032-015-0322-5

2014

Gils M, Sreenivasulu N, Graner A:

Molecular breeding for malting quality. In: Shewry P R, Ullrich S (Eds.): Barley: chemistry and technology, 2nd ed. St. Paul, Minnesota: AACC International Inc. (2014) ISBN 978-1-891127-79-3, 293-310.

Harshavardhan V T, Van Son L, Seiler C, Junker A, Weigelt-Fischer K, Klukas C, Altmann T, Sreenivasulu N, Bäumlein H, Kuhlmann M:

AtRD22 and AtUSPL1, members of the plant-specific BURP domain family involved in Arabidopsis thaliana drought tolerance. PLoS One 9 (2014) e110065. https://dx.doi.org/10.1371/journal.pone.0110065

Kavi Kishor P B, Kalladan R, Reddy P S, Seiler C, Sreenivasulu N:

Drought stress tolerance mechanisms in barley and its relevance to cereals. In: Kumlehn J, Stein N (Eds.): Biotechnological approaches to barley improvement. (Series: Biotechnology in agriculture and forestry, Vol. 69) Berlin: Springer (2014) ISBN 978-3-662-44405-4, 161-179. https://dx.doi.org/10.1007/978-3-662-44406-1_9

Kavi Kishor P B, Sreenivasulu N:

Is proline accumulation per se correlated with stress tolerance or is proline homoeostasis a more critical issue? Plant Cell Environ. 37 (2014) 300-311. https://dx.doi.org/10.1111/pce.12157

Köhler K:

Methylierungsanalyse von ausgewählten DNA-Bereichen zum Funktionsnachweis von AtET2. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften, Institut für Biologie (2014) 44 pp.

Kuhlmann M, Finke A, Mascher M, Mette M F:

DNA methylation maintenance consolidates RNA-directed DNA methylation and transcriptional gene silencing over generations in Arabidopsis thaliana. Plant J. 80 (2014) 269-281. https://dx.doi.org/10.1111/tpj.12630

Reddy P S, Kavi Kishor P B, Seiler C, Kuhlmann M, Eschen-Lippold L, Lee J, Reddy M K, Sreenivasulu N:

Unraveling regulation of the small heat shock proteins by the heat shock factor HvHsfB2c in barley: its implications in drought stress response and seed development. PLoS One 9 (2014) e89125. https://dx.doi.org/10.1371/journal.pone.0089125

Schulze C:

Methylierungsanalyse von ausgewählten DNA-Bereichen zum Funktionsnachweis von AtET1. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften, Institut für Biologie (2014) 37 pp.

Seiler C, Harshavardhan V T, Reddy P S, Hensel G, Kumlehn J, Eschen-Lippold L, Rajesh K, Korzun V, Wobus U, Lee J, Selvaraj G, Sreenivasulu N:

Abscisic acid flux alterations result in differential ABA signalling responses and impact assimilation efficiency in barley under terminal drought stress. Plant Physiol. 164 (2014) 1677-1696. https://dx.doi.org/10.1104/pp.113.229062

2013

Amiteye S, Corral J M, Vogel H, Kuhlmann M, Mette M F, Sharbel T F:

Novel microRNAs and microsatellite-like small RNAs in sexual and apomictic Boechera species. MicroRNA 2 (2013) 45-62. https://dx.doi.org/10.2174/2211536611302010006

Harshavardhan V T:

Altering ABA levels in leaf and seed tissue of barley to study the role of ABA on plant performance under post-anthesis drought stress using the transgenic approach. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2013) 137 pp.

Kalladan R:

Understanding terminal drought tolerance in barley using AB-QTL analysis and an integrated omics approach. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2013) 150 pp.

Kalladan R, Worch S, Rolletschek H, Harshavardhan V T, Kuntze L, Seiler C, Sreenivasulu N, Röder M S:

Identification of quantitative trait loci contributing to yield and seed quality parameters under terminal drought in barley advanced backcross lines. Mol. Breed. 32 (2013) 71-99. https://dx.doi.org/10.1007/s11032-013-9853-9

Koppolu R, Anwar N, Sakuma S, Tagiri A, Lundqvist U, Pourkheirandish M, Rutten T, Seiler C, Himmelbach A, Ariyadasa R, Youssef H M, Stein N, Sreenivasulu N, Komatsuda T, Schnurbusch T:

Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley. Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 13198-13203. https://dx.doi.org/10.1073/pnas.1221950110

Kuhlmann M, Junker A (Eds.):

Barlomics –omic approaches in the barley field. Abstractbook of the 1st IPK Barlomics Summer School 2013. (2013) https://www.ipk-gatersleben.de/index.php?id=1310 pp.

Lermontova I, Kuhlmann M, Friedel S, Rutten T, Heckmann S, Sandmann M, Demidov D, Schubert V, Schubert I:

Arabidopsis KINETOCHORE NULL2 is an upstream component for cenH3 deposition at centromeres. Plant Cell 25 (2013) 3389-3404. https://dx.doi.org/10.1105/tpc.113.114736

Malik Z A, Hensel G, Qureshi J A, Mansoor S, Sreenivasulu N, Kumlehn J, Saeed N A:

Improved agronomic and physiological performance of cultivar “Punjab-11”-derived transgenic wheat under drought stress. Jokull 63 (2013) 136-156.

Mau M, Corral J M, Vogel H, Melzer M, Fuchs J, Kuhlmann M, de Storme N, Geelen D, Sharbel T F:

The conserved chimeric transcript UPGRADE2 is associated with unreduced pollen formation and is exclusively found in apomictic Boechera species. Plant Physiol. 163 (2013) 1640-1659. https://dx.doi.org/10.1104/pp.113.222448

Reddy P S, Sreenivasulu N:

Different omics approaches in cereals and their possible implications for developing a system biology approach to study the mechanism of abiotic stress tolerance. In: Gupta P K, Varshney R K (Eds.): Cereal genomics II. Dordrecht [u.a.]: Springer Netherlands (2013) ISBN 978-94-007-6400-2, 177-214. https://dx.doi.org/10.1007/978-94-007-6401-9_8

Sreenivasulu N, Wobus U:

Seed-development programs: a systems biology-based comparison between dicots and monocots. Annu. Rev. Plant Biol. 64 (2013) 189-217. https://dx.doi.org/10.1146/annurev-arplant-050312-120215

2012

Friedel S, Usadel B, von Wirén N, Sreenivasulu N:

Reverse engineering: a key component of systems biology to unravel global abiotic stress cross-talk. Front. Plant Sci. 3 (2012) 294. https://dx.doi.org/10.3389/fpls.2012.00294

Mir R R, Zaman-Allah M, Sreenivasulu N, Trethowan R, Varshney R K:

Integrated genomics, physiology and breeding approaches for improving drought tolerance in crops. Theor. Appl. Genet. 125 (2012) 625-645. https://dx.doi.org/10.1007/s00122-012-1904-9

Radchuk V, Kumlehn J, Rutten T, Sreenivasulu N, Radchuk R, Rolletschek H, Herrfurth C, Feussner I, Borisjuk L:

Fertility in barley flowers depends on Jekyll functions in male and female sporophytes. New Phytol. 194 (2012) 142-157. https://dx.doi.org/10.1111/j.1469-8137.2011.04032.x

Sreenivasulu N, Harshavardhan V T, Govind G, Seiler C, Kohli A:

Contrapuntal role of ABA: Does it mediate stress tolerance or plant growth retardation under long-term drought stress? Gene 506 (2012) 265-273. https://dx.doi.org/10.1016/j.gene.2012.06.076

Sreenivasulu N, Schnurbusch T:

A genetic playground for enhancing grain number in cereals. Trends Plant Sci. 17 (2012) 91-101. https://dx.doi.org/10.1016/j.tplants.2011.11.003

2011

Govind G, Seiler C, Wobus U, Sreenivasulu N:

Importance of ABA homeostasis under terminal drought stress in regulating grain filling events. Plant Signal. Behav. 6 (2011) 1228-1231. https://dx.doi.org/10.4161/psb.6.8.16254

Kock V:

Salinity tolerance mechanisms in barley. Internship plant breeding thesis. (Master Thesis) Wageningen/The Netherlands, Wageningen UR-Plant Breeding (2011) 44 pp.

Lata C, Jha S, Dixit V, Sreenivasulu N, Prasad M:

Differential antioxidative responses to dehydration-induced oxidative stress in core set of foxtail millet cultivars [Setaria italica (L.)]. Protoplasma 248 (2011) 817-828. https://dx.doi.org/10.1007/s00709-010-0257-y

Melkus G, Rolletschek H, Fuchs J, Radchuk V, Grafahrend-Belau E, Sreenivasulu N, Rutten T, Weier D, Heinzel N, Schreiber F, Altmann T, Jakob P M, Borisjuk L:

Dynamic (13) C/(1) H NMR imaging uncovers sugar allocation in the living seed. Plant Biotechnol. J. 9 (2011) 1022-1037. https://dx.doi.org/10.1111/j.1467-7652.2011.00618.x

Puranik S, Jha S, Srivastava P S, Sreenivasulu N, Prasad M:

Comparative transcriptome analysis of contrasting foxtail millet cultivars in response to short-term salinity stress. J. Plant Physiol. 168 (2011) 280-287. https://dx.doi.org/10.1016/j.jplph.2010.07.005

Seiler C, Harshavardhan V T, Kalladan R, Reddy P S, Strickert M, Rolletschek H, Scholz U, Wobus U, Sreenivasulu N:

ABA biosynthesis and degradation contributing to ABA homeostasis during barley seed development under control and terminal drought-stress conditions. J. Exp. Bot. 62 (2011) 2615-2632. https://dx.doi.org/10.1093/jxb/erq446

Worch S, Kalladan R, Harshavardhan V T, Pietsch C, Korzun V, Kuntze L, Börner A, Wobus U, Röder M S, Sreenivasulu N:

Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality. BMC Plant Biol. 11 (2011) 1. https://dx.doi.org/10.1186/1471-2229-11-1

2010

Lohse M, Nunes-Nesi A, Krüger P, Nagel A, Hannemann J, Giorgi F M, Childs L, Osorio S, Walther D, Selbig J, Sreenivasulu N, Stitt M, Fernie A R, Usadel B:

Robin: an intuitive wizard application for R-based expression microarray quality assessment and analysis. Plant Physiol. 153 (2010) 642-651. https://dx.doi.org/10.1104/pp.109.152553

Sharma S, Sreenivasulu N, Harshavardhan V T, Seiler C, Sharma S, Khalil Z N, Akhunov E, Sehgal S K, Röder M S:

Delineating the structural, functional and evolutionary relationships of sucrose phosphate synthase gene family II in wheat and related grasses. BMC Plant Biol. 10 (2010) Article ID: 134. https://dx.doi.org/10.1186/1471-2229-10-134

Sreenivasulu N, Borisjuk L, Junker B H, Mock H-P, Rolletschek H, Seiffert U, Weschke W, Wobus U:

Barley grain development toward an integrative view. Int. Rev. Cell Mol. Biol. 281 (2010) 49-89. https://dx.doi.org/10.1016/S1937-6448(10)81002-0

Sreenivasulu N, Radchuk V, Alawady A, Borisjuk L, Weier D, Staroske N, Fuchs J, Miersch O, Strickert M, Usadel B, Wobus U, Grimm B, Weber H, Weschke W:

De-regulation of abscisic acid contents causes abnormal endosperm development in the barley mutant seg8. Plant J. 64 (2010) 589-603. https://dx.doi.org/10.1111/j.1365-313X.2010.04350.x

Sreenivasulu N, Röder M S, Wobus U:

Trockenstress – eine Suche nach den Ursachen und nach neuen Wegen zur Züchtung trockentoleranter Getreide. GenomXPress 4 (2010) 4-6.

Sreenivasulu N, Sunkar R, Wobus U, Strickert M:

Array platforms and bioinformatics tools for the analysis of plant transcriptome in response to abiotic stress. In: Sunkar R (Ed.): Plant stress tolerance: methods and protocols. (Series: Methods in molecular biology, Vol. 639) New York [u.a.]: Humana Press (2010) ISBN 978-1-60761-701-3, 71-93. https://dx.doi.org/10.1007/978-1-60761-702-0_5

Weber H, Sreenivasulu N, Weschke W:

Molecular physiology of seed maturation and seed storage protein biosynthesis. In: Pua E-C, Davey M R (Eds.): Plant developmental biology - biotechnological perspectives. Vol. 2. Heidelberg [u.a.]: Springer (2010) ISBN 978-3-642-04669-8, 83-104. https://dx.doi.org/10.1007/978-3-642-04670-4_5

Weichert N, Saalbach I, Weichert H, Kohl S, Erban A, Kopka J, Hause B, Varshney A, Sreenivasulu N, Strickert M, Kumlehn J, Weschke W, Weber H:

Increasing sucrose uptake capacity of wheat grains stimulates storage protein synthesis. Plant Physiol. 152 (2010) 698-710. https://dx.doi.org/10.1104/pp.109.150854

2009

Govind G, Harshavardhan V T, Patricia J K, Dhanalakshmi R, Senthil Kumar M, Sreenivasulu N, Udayakumar M:

Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut. Mol. Genet. Genomics 281 (2009) 591-605. https://dx.doi.org/10.1007/s00438-009-0432-z

Pietsch C, Sreenivasulu N, Wobus U, Röder M S:

Linkage mapping of putative regulator genes of barley grain development characterized by expression profiling. BMC Plant Biol. 9 (2009) 4. https://dx.doi.org/10.1186/1471-2229-9-4

Radchuk V V, Borisjuk L, Sreenivasulu N, Merx K, Mock H-P, Rolletschek H, Wobus U, Weschke W:

Spatiotemporal profiling of starch biosynthesis and degradation in the developing barley grain. Plant Physiol. 150 (2009) 190-204. https://dx.doi.org/10.1104/pp.108.133520

2008

Balyan H S, Sreenivasulu N, Riera-Lizarazu O, Azhaguvel R, Kianian S F:

Mutagenesis and high-throughput functional genomics in cereal crops: Current status. Adv. Agron. 98 (2008) 357-414. https://doi.org/10.1016/S0065-2113(08)00207-1

Neuberger T, Sreenivasulu N, Rokitta M, Rolletschek H, Göbel C, Rutten T, Radchuk V, Feussner I, Wobus U, Jakob P, Webb A, Borisjuk L:

Quantitative imaging of oil storage in developing crop seeds. Plant Biotechnol. J. 6 (2008) 31-45. https://dx.doi.org/10.1111/j.1467-7652.2007.00294.x

Radchuk V, Sreenivasulu N, Blume Y, Weschke W:

Cloning and expression of the tubulin genes in barley. Cell Biol. Int. 32 (2008) 557-559. https://dx.doi.org/10.1016/j.cellbi.2007.11.009

Sreenivasulu N, Graner A, Wobus U:

Barley genomics: an overview. Int. J. Plant Genomics (2008) 13 pp. https://dx.doi.org/10.1155/2008/486258

Sreenivasulu N, Pietsch C, Radchuk V V, Strickert M, Röder M, Weschke W, Wobus U:

Regulators determining seed maturation: a genetical genomics approach. In: Molina-Cano J L, Christou P, Graner A, Hammer K, Jouve N, Keller B, Lasa J M, Powell W, Royo C, Shewry P, Stanca A M (Eds.): Cereal science and technology for feeding ten billion people: genomics era and beyond, Proceedings for the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13.-17.11.2006. (Series: Options Méditerranéennes, Series A, Séminaires Méditerranéens, Vol. 81) Zaragoza: CIHEAM / IRTA (2008) ISBN 2-85352-404-3, 431-434.

Sreenivasulu N, Usadel B, Winter A, Radchuk V, Scholz U, Stein N, Weschke W, Strickert M, Close T J, Stitt M, Graner A, Wobus U:

Barley grain maturation and germination: metabolic pathway and regulatory network commonalities and differences highlighted by New MapMan/PageMan profiling tools. Plant Physiol. 146 (2008) 1738-1758. https://dx.doi.org/10.1104/pp.107.111781

Strickert M, Sreenivasulu N, Villmann T, Hammer B:

Robust centroid-based clustering using derivatives of Pearson correlation. In: Encarnacao P, Veloso A (Eds.): Proceedings of the First International Conference on Bio-inspired Systems and Signal Pocessing; Funchal, Madeira, Portugal, January 28 - 31, 2008, Vol. 2. [S.I]: INSTICC Press (2008) ISBN 978-989-8111-18-0, 197-203. https://dx.doi.org/

Thiel J, Weier D, Sreenivasulu N, Strickert M, Weichert N, Melzer M, Czauderna T, Wobus U, Weber H, Weschke W:

Different hormonal regulation of cellular differentiation and function in nucellar projection and endosperm transfer cells - a microdissection-based transcriptome study of young barley grains. Plant Physiol. 148 (2008) 1436-1452. https://dx.doi.org/10.1104/pp.108.127001

2007

Radchuk V V, Sreenivasulu N, Blume Y, Weschke W:

Distinct tubulin genes are differentially expressed during barley grain development Physiol. Plant. 131 (2007) 571-580. https://dx.doi.org/10.1111/j.1399-3054.2007.00976.x

Sreenivasulu N, Sopory S K, Kavi Kishor P B:

Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene 388 (2007) 1-13. https://dx.doi.org/10.1016/j.gene.2006.10.009

Strickert M, Sreenivasulu N, Usadel B, Seiffert U:

Correlation-maximizing surrogate gene space for visual mining of gene expression patterns in developing barley endosperm tissue. BMC Bioinformatics 8 (2007) 165. https://dx.doi.org/10.1186/1471-2105-8-165

Weschke W, Mock H-P, Pietsch C, Radchuk V, Röder M S, Schreiber F, Seiffert U, Sreenivasulu N, Strickert M, Witzel K, Wobus U:

Genetical Genomics der Gerstenkornentwicklung - von der Genexpression zu landwirtschaftlich bedeutsamen Merkmalen. GenomXPress 1 (2007) 12-16.

Wobus U, Mock H-P, Pietsch C, Radchuk V, Röder M, Schreiber F, Seiffert U, Sreenivasulu N, Strickert M, Weschke W, Witzel K:

GABI-SEED: Genetische Grundlagen komplexer agronomischer Merkmale im Getreidekorn entschlüsseln. GenomXPress Sonderausgabe März (2007) 19.

2006

Jogeswar G, Pallela R, Jakka N M, Reddy P S, Rao J V, Sreenivasulu N, Kishor P B K:

Antioxidative response in different sorghum species under short-term salinity stress. Acta Physiol. Plant. 28 (2006) 465-475. https://dx.doi.org/10.1007/BF02706630

Sreenivasulu N, Radchuk V, Strickert M, Miersch O, Weschke W, Wobus U:

Gene expression patterns reveal tissue-specific signaling networks controlling programmed cell death and ABA- regulated maturation in developing barley seeds. Plant J. 47 (2006) 310-327. https://dx.doi.org/10.1111/j.1365-313X.2006.02789.x

Strickert M, Seiffert U, Sreenivasulu N, Weschke W, Villmann T, Hammer B:

Generalized relevance LVQ (GRLVQ) with correlation measures for gene expression analysis. Neurocomputing 69 (2006) 651-659. https://dx.doi.org/10.1016/j.neucom.2005.12.004

Strickert M, Sreenivasulu N, Peterek S, Weschke W, Mock H-P, Seiffert U:

Unsupervised feature selection for biomarker identification in chromatography and gene expression data. In: Schwenker F, Marinai S (Eds.): Artificial neural networks in pattern recognition: second IAPR Workshop, ANNPR 2006, Ulm Germany, August 31 - September 2, 2006. proceedings. (Series: Lecture Notes in Computer Science, Vol. 4087) Berlin [u.a.]: Springer (2006) ISBN 978-3-540-37951-5, 274-285. https://dx.doi.org/10.1007/11829898_25

Strickert M, Sreenivasulu N, Seiffert U:

Sanger-driven MDSLocalize - a comparative study for genomic data. In: Verleysen M (Ed.): Advances in computational intelligence and learning: 14th European Symposium on Artificial Neural Networks, ESANN 2006, Bruges, Belgium, April 26 - 28, 2006, proceedings. Evere, Belgium: d-side (2006) ISBN 2-930307-06-4, 265-270.

Usadel B, Nagel A, Steinhauser D, Gibon Y, Blasing O E, Redestig H, Sreenivasulu N, Krall L, Hannah M A, Poree F, Fernie A R, Stitt M:

PageMan: an interactive ontology tool to generate, display, and annotate overview graphs for profiling experiments. BMC Bioinformatics 7 (2006) 535. https://dx.doi.org/10.1186/1471-2105-7-535

Wobus U, Sreenivasulu N:

Genomics approaches for the improvement of cereals. In: Freitag J (Ed.): Plant genomics and bioinformatics expression micro arrays and beyond: a course book (European Training and Networking Activity). Potsdam-Golm: MPI-MPP (2006) ISBN 3-00-018316-7, 146-155.